CNTF Antibody

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  • Rabbit anti Rat CNTF
  • Rabbit anti Rat CNTF:Biotin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AAR21C, E, FN, IF, WBdatasheet pdfdatasheet pdf0.1 mg
    AAR21
    AAR21BE, WBdatasheet pdfdatasheet pdf50 µg
    AAR21B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Rat CNTF antibody recognizes rat ciliary neurotrophic factor (CNTF)..
      .
      Rat CNTF is a 22.7kD neuroregulatory cytokine which is found primarily in peripheral nerve tissue. CNTF promotes the survival and differentiation of neurons and other neuronal cell types.
    • Intended Use
    • Target Species
      Rat
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - lyophilised
      Purified IgG conjugated to Biotin - lyophilised
    • Reconstitution
      Reconstitute with 0.1 ml distilled water.

      The addition of 0.09% sodium azide is recommended for long term storage.

      N.B. For functional studies do not add sodium azide.
      Reconstitute with 0.5 ml sterile PBS containing 0.1% Bovine Serum Albumin.

      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.

      The addition of 0.09% sodium azide is recommended for long term storage
    • Preparation
    • Antiserum Preparation
      Antisera to rat CNTF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      None present
      None Present
    • Immunogen
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml after reconstitution
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • GO Terms
      neuronal cell body
      growth factor activity
      positive regulation of phosphatidylinositol 3-kinase cascade
      positive regulation of axon regeneration
      negative regulation of neuron apoptosis
      axon
      JAK-STAT cascade
      positive regulation of hormone secretion
      positive regulation of cell growth
      positive regulation of MAPKKK cascade
      cell projection cytoplasm
      positive regulation of tyrosine phosphorylation of Stat3 protein
      protein heterooligomerization
      ciliary neurotrophic factor receptor binding
      positive regulation of cell proliferation
      growth
      cytosol
      regulation of resting membrane potential
      astrocyte activation
      cytokine activity
      neuroprotection
      membrane hyperpolarization
      nucleus
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.52.0ug/ml
      Functional Assays20ng/ml50ng/ml
      Immunofluorescence
      Immunohistology - Frozen
      Western Blotting0.1ug/ml0.2ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.25ug/ml1.0ug/ml
      Western Blotting0.1ug/ml0.2ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems with the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with AAR21B as the detection antibody and PRP26 as the standard.
    • ELISA
      This antibody may be used in a direct ELISA system or as a detection reagent in a sandwich ELISA together with AAR21 as the capture reagent and PRP26 as the standard.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CNTF Antibody Formats

    Formats Applications Sizes available
    CNTF Antibody : Biotin E, WB 50 µg
    CNTF Antibody : Purified C, E, FN, IF, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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      STAR121B
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      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
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      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              References

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