Toxoplasma gondii Antibody

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Published customer image:
Rabbit anti Toxoplasma gondii RH Strain antibody used for the identification of Toxoplasma cysts by immunofluorescence.
Image caption:
Compound 2 enhances differentiation. A. Structure of Compound 2. B. Immunofluorescence of a cyst-like structure using an anti-Toxoplasma antibody (green) to detect all parasites and Dolichos biflorus lectin (DL; red) to detect the cyst wall after 72 hr under CO2 starvation conditions. The corresponding differential interference contrast (DIC) and merged images are also shown. Scale bar = 10μm. C. Percentage of DL-positive vacuoles (DL+) in samples treated with either Compound 2 (3μM) or an equivalent volume of DMSO for 72 hr under CO2 starvation conditions (mean ± SD; n = 4 for the wild-type strain and n = 6 for &DELTA;uprt strains). The data were compared using paired Student's t-test (**p < 0.01).

From: Odell AV, Tran F, Foderaro JE, Poupart S, Pathak R, Westwood NJ, et al. (2015) Yeast Three-Hybrid Screen Identifies TgBRADIN/GRA24 as a Negative Regulator of Toxoplasma gondii Bradyzoite Differentiation.
PLoS ONE 10(3): e0120331.

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  • Rabbit anti Toxoplasma gondii RH Strain
  • Rabbit anti Toxoplasma gondii:Biotin
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    9070-0556IFdatasheet pdfdatasheet pdf1 ml
    9070-0556
    9070-0606IFdatasheet pdfdatasheet pdf1 ml
    9070-0606
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Toxoplasma gondii RH Strain antibody recognizes purified Toxoplasma gondii trophozoites.

      T. gondii is an intracellular protozoan parasite that enters its host via the gastrointestinal tract, and is the only member of the Toxoplasma genus. The definitive host of T. gondii is cats, but it can also be carried by a vast number of warm-blooded animals, including humans. Toxoplasmosis is usually minor and self-limiting but can have serious or even fatal effects on a fetus, or if the host is immunocompromised. T. gondii encephalitis (TE) is the most common cerebral opportunistic infection in patients with AIDS.
    • Intended Use
    • Target Species
      Protozoan
    • Product Form
      Purified IgG - liquid
      Purified Ig conjugated to Biotin - liquid
    • Reconstitution
    • Preparation
      Purified Ig prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      0.1%Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Toxoplasma gondii RH strain.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 4.0 mg/ml
      Ig concentration 4.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence
    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence1/1000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Toxoplasma gondii Antibody Formats

    Formats Applications Sizes available
    Toxoplasma gondii Antibody : Biotin IF 1 ml
    Toxoplasma gondii Antibody : Purified IF 1 ml
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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