Romosozumab antibody | AbD60141ad
Human anti Romosozumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD60141ad
- Isotype
- Fab antibody
- Specificity
- Romosozumab
Antibody Quality and Performance Guarantee
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Romosozumab Antibody, clone AbD60141ad (TZA060) at a concentration of 2 μg/ml followed by HRP conjugated Anti-DYKDDDDK Tag Antibody in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate.
A microtiter plate was coated overnight with romosozumab, Sclerostin-His, human IgG2 kappa control and normal human serum (NHS) at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Romosozumab Antibody, clone AbD60141ad (TZA060) titrated to the given concentrations followed by an HRP conjugated Anti-DYKDDDDK Tag Antibody in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate. Data for romosozumab are shown as the mean of three measurements.
A microtiter plate was coated overnight with Human Anti-Romosozumab Antibody, clone AbD60141ad (TZA060) at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of romosozumab. Detection was performed using HRP-conjugated Human Anti-Romosozumab Antibody, clone AbD60146pap (TZA061P) at a concentration of 0.5 µg/mL in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate. Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with recombinant human Sclerostin-His at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, a pre-incubated mixture of romosozumab (0.15 µg/ml) plus increasing concentrations of Human Anti-Romosozumab Antibody, clone AbD60141ad (TZA060). Free romosozumab, still capable of binding to the Sclerostin-His coated plate, was detected using HRP conjugated Mouse Anti-Human IgG (Fc) CH2 Domain Antibody (MCA647P) in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu fluorogenic peroxidase substrate. Data are shown as the mean of three measurements.
Filter by Application:
E Reset| Human Anti-Romosozumab Antibody, clone AbD60141ad is a paratope specific, inhibitory, anti-idiotypic antibody (Type 1) that specifically recognizes the free human monoclonal antibody drug romosozumab, and its biosimilar products. It does not recognize the drug target, sclerostin, nor romosozumab in complex with sclerostin. This antibody can be used to measure free romosozumab levels in serum from patients. A pair of anti-romosozumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug; clone AbD60141ad is recommended as a capture antibody, paired with Human Anti-Romosozumab Antibody, clone AbD60146 (TZA061) as the detection antibody. TZA060 is a monovalent Fab antibody incorporating a SpyTag at the C-terminus end of the antibody heavy chain for conversion by the end user into alternative formats using any of the SpyCatchers available in our catalog. Romosozumab (trade name Evenity) is a humanized monoclonal antibody of the IgG2/kappa isotype that binds to sclerostin and prevents inhibition of the Wnt signaling pathway, thereby promoting osteogenesis and, to a lesser extent, reducing bone resorption. Romosozumab's inhibition of sclerostin also inhibits the increase in RANKL-dependent increases in osteoclast activity and bone resorption. Romosozumab is indicated for the treatment of osteoporosis in postmenopausal women at high risk for fracture. View a summary of all anti-romosozumab antibodies |
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in a proprietary E. coli. strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.0095% MIT
- Immunogen
- Romosozumab
- Affinity
- The monovalent intrinsic affinity of AbD60141ad was measured as KD = 0.7 nM by real time, label free molecular interaction analysis on immobilized romosozumab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences. Evenity is a trademark of Amgen and UCB. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Clone AbD60141ad can be used in indirect ELISA to detect romosozumab. It can also be used as a capture antibody to develop a pharmacokinetic (PK) bridging ELISA for romosozumab together with TZA061 or TZA061P as the detection reagent.
Protocol: PK bridging ELISA
- Synonyms
- Evenity
TZA060
171356
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with Romosozumab specificity
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