YTE Mutations antibody | AbD64789
Human anti YTE Mutations
- Product Type
- Monoclonal Antibody
- Clone
- AbD64789
- Isotype
- Fab antibody
- Specificity
- YTE Mutations
Antibody Quality and Performance Guarantee
TZA0136
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/mL, including Efgartigimod, Nirsevimab and AbD18705iu, which carry the YTE mutations. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-YTE Mutations Antibody, clone AbD64789ad (TZA0136) at a concentration of 2 μg/mL followed by HRP conjugated Anti-DYKDDDDK Tag Antibody in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate.
A microtiter plate was coated overnight with AbD18705iu carrying the YTE mutations, as well as human IgG1, human IgG4 pro control, and cyno IgG as controls without the YTE mutations at a concentration of 5 µg/mL. After washing and blocking with PBST + 5% BSA, detection was performed using HRP conjugated Human Anti-YTE Mutations Antibody, clone AbD64789pap (TZA0136P) titrated to the given concentrations in HISPEC diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data for AbD18705iu are shown as the mean of two measurements.
A microtiter plate was coated overnight with Human Anti-YTE mutations Antibody, clone AbD64785ad (TZA0135) at a concentration of 1 µg/mL. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of Recombinant Human IgG1 YTE Kappa Allotype G1m3 ((HCA413) carrying the YTE-mutations. Detection was performed using HRP-conjugated Human Anti-YTE mutations Antibody, clone AbD64789pap (TZA0136P) at a concentration of 0.5 µg/mL in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate. Data are shown as the mean of three measurements.
Filter by Application:
E Reset| Human Anti-YTE mutation Antibody, clone AbD64789 is an antibody that specifically recognizes the YTE mutation (M252Y/S254T/T256E) in human IgG1 antibodies. This antibody can be used to measure antibody drug concentrations in serum from patients treated with antibody drugs carrying this YTE mutation. For PK assays, clone AbD64789 is recommended as a detection antibody, paired with Human anti-YTE mutation antibody, clone AbD64785ad (TZA0135) as the capture antibody. Clone AbD64789 is available as a monovalent Fab antibody incorporating a SpyTag at the C-terminus end of the antibody heavy chain for conversion by the end user into alternative formats using any of the SpyCatchers available in our catalog under product code TZA0136. It is also available as a bivalent Fab antibody already conjugated to HRP via coupling to the HRP conjugated BiSpyCatcher2 (TZC002P) for use in direct ELISA under product code TZA0136P. The YTE-mutations (M252Y/S254T/T256E) were initially introduced at the CH2-CH3 interface of the humanized IgG1 drug antibody MEDI-524 (anti-respiratory syncytial virus, RSV). It was shown that these mutations lead to a prolonged half time in serum due to a higher affinity to the neonatal Fc receptor (FcRn) and is therefore an interesting format for many antibody drugs. View a summary of all anti-yte mutation antibodies |
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in a proprietary E. coli. strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.0095% MIT
- Immunogen
- YTE mutations (AbD18705iu, hIgG1 carrying the YTE mutations)
- Affinity
- The monovalent intrinsic affinity of AbD64789ad was measured as KD = 3 nM by real time, label free molecular interaction analysis on immobilized AbD18705iu.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Clone AbD64789ad can be used in indirect ELISA for AbD18705iu and other antibodies carrying the YTE mutations. It can also be used as a detection antibody to develop a pharmacokinetic (PK) bridging ELISA together with TZA0135 as the capture reagent. Note: This antibody has only low affinity towards YTE in the presence of additional Fc mutations. For antibodies carrying additional mutations we recommend to use our alternative PK pair of TZA0137 and TZA0137P.
Protocol: PK bridging ELISA
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
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| Description | Hispec Assay Diluent | ||||||
| Recombinant Human IgG1 YTE Kappa Allotype G1m3 | HCA413 | E | 0.1 mg |
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| Description | Recombinant Human IgG1 YTE Kappa Allotype G1m3 | ||||||
| Human anti YTE Mutations | TZA0135 | E | 0.1 mg |
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| Description | Human anti YTE Mutations | ||||||
| Human anti YTE Mutations | TZA0136P | E | 0.1 mg |
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| Description | Human anti YTE Mutations | ||||||
| Human anti YTE Mutations | TZA0137 | E | 0.1 mg |
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| Description | Human anti YTE Mutations | ||||||
| Human anti YTE Mutations | TZA0137P | E | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Human anti YTE Mutations | ||||||
TZA0136
172597
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