Dulaglutide antibody | AbD64818
Human anti Dulaglutide:HRP
- Product Type
- Monoclonal Antibody
- Clone
- AbD64818
- Isotype
- Fab antibody
- Specificity
- Dulaglutide
Antibody Quality and Performance Guarantee
TZA0123P
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/mL, including the GLP-1 BSA complex, GLP-1 transferrin complex and GLP-1 IgG4-Fc fusion protein dulaglutide. After washing and blocking with PBST + 5% BSA, detection was performed using HRP conjugated Human Anti-Dulaglutide Antibody, clone AbD64818pap (TZA0123P) at a concentration of 0.2 μg/mL in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate.
A microtiter plate was coated overnight with dulaglutide, human GLP-1 coupled to Transferrin, human IgG4-pro isotype control, human IgG4-LALA isotype control, and galcanezumab at a concentration of 5 µg/mL. After washing and blocking with PBS + 5% BSA, detection was performed using Human Anti-Dulaglutide Antibody, clone AbD64818ad (TZA0123) titrated to the given concentrations followed by an HRP conjugated Anti-DYKDDDDK Tag Antibody in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate. Data for dulaglutide are shown as the mean of three measurements.
A microtiter plate was coated overnight with Human Anti-Dulaglutide Antibody, clone AbD64818ad (TZA0123) at a concentration of 1 µg/mL. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of dulaglutide. Detection was performed using HRP-conjugated Human Anti-Dulaglutide Antibody, clone AbD64818pap (TZA0123P) at a concentration of 0.5 µg/mL in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate. Data are shown as the mean of three measurements.
Filter by Application:
E Reset| Human Anti-Dulaglutide Antibody, clone AbD64818 is specific for human GLP-1 and its peptidase-IV-protected analog present in the human IgG4-Fc fusion protein dulaglutide. Clone AbD64818 is available as a monovalent Fab antibody incorporating a SpyTag at the C-terminus end of the antibody heavy chain for conversion by the end user into alternative formats using any of the SpyCatchers available in our catalog under product code TZA0123. It is also available as a bivalent Fab antibody already conjugated to HRP via coupling to the HRP conjugated BiSpyCatcher2 (TZC002P) for use in direct ELISA under product code TZA0123P. A pair of anti-dulaglutide antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug; clone AbD64818ad is recommended as a capture antibody, paired with Human Anti-Dulaglutide Antibody, clone AbD64818pap (TZA0123P) as the detection reagent. Dulaglutide (trade name Trulicity) is a fusion protein, combining a modified GLP-1 analogue with a human IgG4-Fc heavy chain. It binds to GLP-1 and activates the GLP-1 receptor found in the pancreatic beta cells increasing intracellular cyclic AMP in beta cells, leading to insulin release and subsequent reduction of blood glucose concentrations. Dulaglutide is indicated for the management of type 2 diabetes and reduce major adverse cardiovascular events. View a summary of all anti-dulaglutide antibodies |
- Product Form
- A bivalent human recombinant Fab (lambda light chain) generated by coupling of the monovalent SpyTagged Fab TZA0123 with a HRP conjugated BiSpyCatcher version 2. The coupled antibody has two DYKDDDDK tags and three His6-tags. It contains SpyTag3 peptide (BLP086) in 5-fold molar excess to block any unreacted Catcher sites. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.0095% MIT
- Immunogen
- Dulaglutide
- Affinity
- The monovalent intrinsic affinity of AbD64818pap was measured as KD = 2.0 nM by real time, label free molecular interaction analysis on immobilized dulaglutide.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Trulicity is a trademark of Eli Lily. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
This product is shipped frozen.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Clone AbD64818pap can be used as a detection antibody to develop a pharmacokinetic (PK) bridging ELISA for dulaglutide, together with TZA0123 as the capture reagent.
Protocol: PK bridging ELISA
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Dulaglutide | TZA0123 | E | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Human anti Dulaglutide | ||||||
- Synonyms
- Trulicity
TZA0123P
172594
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with Dulaglutide specificity
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