Anti-Dulaglutide Antibodies

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Antibodies for bioanalysis and drug monitoring of dulaglutide

Develop highly selective and sensitive PK and ADA assays for the GLP-1 receptor agonist dulaglutide (Trulicity®) using our range of ready-made antibodies.

• Fully human surrogate positive control or calibrator
• Sequence defined, well characterized reagents with secure supply
• Recombinant production, stringent quality control for batch-to-batch consistency
• Non-animal-derived antibodies, reducing the use of animals in science

Table 1. Antibodies Specific to Dulaglutide.

* Affinity measured in the monovalent Fab format.
¹ Fab-F-Spy2-H = Monovalent Fab antibody with (F) DYKDDDDK, (Spy2) SpyTag version 2, and (H) His-6-tags.
² Fab2-FH-X22-HRP = Bivalent Fab antibody with (F) DYKDDDDK, (H) His-6-tags, and Fab-SpyTag coupled to HRP-conjugated BiCatcher.

Anti-Dulaglutide Antibodies

Dulaglutide (Trulicity) is a GLP‑1 receptor agonist made of a GLP‑1 peptide fused to a human IgG4‑Fc fragment. The Fc part has the IGHG4*04 allotype containing a LALA mutation. Dulaglutide activates the GLP-1 receptor found in pancreatic beta cells, increasing intracellular cyclic adenosine monophosphate (cAMP) and stimulating insulin release, subsequently reducing blood glucose concentration. Additionally, dulaglutide decreases glucagon secretion and slows gastric emptying.

Anti-Dulaglutide Antibodies

Anti-dulaglutide antibodies, clones AbD64818ad and AbD64818ia, are recombinant antibodies that are specific for GLP-1 and recognize the free GLP-1/IgG4-Fc fusion protein, dulaglutide. These clones also recognize human GLP-1 coupled to carrier proteins.

They are ideal for the development of a pharmacokinetic (PK) bridging ELISA to measure free drug. In a fully human IgG1 format, they are suitable as a surrogate positive control or calibrator for an anti-drug antibody (ADA) assay.

ELISA Protocols to Get You Started

PK Bridging ELISA

Fig. 1. Dulaglutide PK bridging ELISA using antibodies TZA0123 (capture) and TZA0123P (detection).

Schematic image of PK bridging ELISA measuring free drug. Capture antibody, Fab format (purple), fusion protein drug (gold-green), detection antibody, Fab format (purple), labeled with HRP.

ADA Bridging ELISA

Fig. 2. Dulaglutide ADA bridging ELISA using antibodies using HCA449.

Schematic image of ADA bridging assay. Fusion protein drug as capture and detection reagent labeled with HRP (gold-green), fully human anti-drug antibody, Ig format (blue).

Resources

Licensed Use: For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.