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CD25 antibody | P4A10

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Anti Dog CD25 Antibody, clone P4A10 thumbnail image 1 Anti Dog CD25 Antibody, clone P4A10 thumbnail image 2
Anti Dog CD25 Antibody, clone P4A10 gallery image 1
Figure A. APC conjugated Rat anti Dog CD4 (MCA1038APC) and FITC conjugated Mouse IgG1 isotype control (MCA928F) Figure B. APC conjugated Rat anti Dog CD4 (MCA1038APC) and FITC conjugated Mouse anti Dog CD25 (MCA5916F). All experiments performed on red cell lysed canine blood gated lymphoid cells in the presence of 10% dog serum. Data acquired on the ZE5 Cell Analyzer.
Anti Dog CD25 Antibody, clone P4A10 gallery image 2
Figure A. PE conjugated Mouse anti Canine CD25 (MCA5916PE) and FITC conjugated Rat IgG2c isotype control. Figure B. PE conjugated Mouse anti Canine CD25 (MCA5916F) and FITC conjugated Rat anti Canine CD11/18. All experiments performed on red cell lysed canine blood gated on mononuclear cells.

Mouse anti Dog CD25:RPE

Product Type
Monoclonal Antibody
Clone
P4A10
Isotype
IgG1
Specificity
CD25

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Product Code Applications Pack Size List Price Your Price Qty
MCA5916PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price
loader

Mouse anti Dog CD25, clone P4A10 recognizes the canine homologue of the human CD25 cell surface antigen, also known at IL-2Rα, a glycoprotein of approximately 55 kDa expressed primarily by activated T lymphocytes (Abrams et al. 2010).

The IL-2 receptor is composed of 3 subunits, an α chain (CD25), a β chain (CD122) and a γ chain (CD132), CD25 functions as a low affinity receptor for IL-2.

Antibodies to CD4,

Mouse anti Human CD25 antibody, clone ACT1, which has demonstrated cross reactivity to canine CD25 (Rissetto et al. 2010). However this clone has poor affinity for canine CD25. The development of clone P4A10 offers a specific monoclonal Mouse anti Canine CD25, demonstrating a greater affinity for canine CD25 than the cross reactive anti human antibody (Abrams et al. 2010).

The dog is an important veterinary species in its own right. In addition dogs are used as an animal model in the study of a number of serious human disease states including various forms cancers (Paoloni et al. 2008), and in genetically related diseases of the hemopoietic system (Bauer Jr.et al. 2009).

Target Species
Dog
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Immunogen
Enriched and stimulated canine T cells.
Fusion Partners
Spleen cells from immunized RBF/DnJ mice were fused with cells of the mouse P3-653 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests
>
List Price Your Price
Description Mouse IgG1 Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat anti Dog CD4 MCA1038GA C F 0.1 mg loader
List Price Your Price
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Description Rat anti Dog CD4
Rat anti Dog CD8 MCA1039GA C F IF 0.1 mg loader
List Price Your Price
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Description Rat anti Dog CD8
Mouse anti Dog CD3:FITC MCA1774F F 0.1 mg loader
List Price Your Price
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Description Mouse anti Dog CD3:FITC
Mouse anti Dog CD3 MCA1774GA C F IF IP 0.1 mg loader
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Description Mouse anti Dog CD3

References for CD25 antibody

  1. Abrams, V.K. et al. (2010) A novel monoclonal antibody specific for canine CD25 (P4A10): selection and evaluation of canine Tregs.
    Vet Immunol Immunopathol. 135 (3-4): 257-65.
  2. Finn, J.D. et al. (2010) Eradication of neutralizing antibodies to factor VIII in canine hemophilia A after liver gene therapy.
    Blood.116: 5842-8.
  3. Mizutani, N. et al. (2020) Measurement of the concentration of serum soluble interleukin-2 receptor alpha chain in dogs with lymphoma.
    Vet Immunol Immunopathol. 225: 110054.
  4. Wesolowski, M. et al. (2023) Long-term changes of Th17 and regulatory T cells in peripheral blood of dogs with spinal cord injury after intervertebral disc herniation.
    BMC Vet Res. 19 (1): 90.
  5. Sheng, R. et al. (2023) Prognostic significance of CD25 expression in dogs with a noninvasive diagnosis of B-cell lymphoma treated with CHOP chemotherapy.
    Vet Comp Oncol. 21 (1): 28-35.

Further Reading

  1. Paoloni, M. & Khanna, C. (2008) Translation of new cancer treatments from pet dogs to humans.
    Nat Rev Cancer. 8 (2): 147-56.
  2. Bauer, T.R. Jr et al. (2009) Potential large animal models for gene therapy of human genetic diseases of immune and blood cell systems.
    ILAR J. 50 (2): 168-86.
  3. Rissetto, K.C. et al. (2010) Cloning and expression of canine CD25 for validation of an anti-human CD25 antibody to compare T regulatory lymphocytes in healthy dogs and dogs with osteosarcoma.
    Vet Immunol Immunopathol. 135 (1-2): 137-45.
  4. Pinheiro, D. et al. (2011) Phenotypic and functional characterization of a CD4(+) CD25(high) FOXP3(high) regulatory T-cell population in the dog.
    Immunology. 132 (1): 111-22.

Flow Cytometry

Western Blotting

Synonyms
IL-2R Alpha Chain
UniProt
O62802
Entrez Gene
IL2RA
GO Terms
GO:0016021 integral to membrane

MCA5916PE

167396 INN1804

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