CD62L antibody | CC32
Mouse anti Bovine CD62L antibody, clone CC32 (MCA1649G) used for the evaluation of L-selectin expression on PBMC from M. bovis cattle by flow cytometry.
Image caption:
Characterisation of memory markers reveals a characteristic TEM phenotype for multifunctional CD4+ cells in naturally infected cattle. PBMC from naturally M. bovis infected cattle were stimulated with PPD-B and stained for production of cytokine by ICS flow cytometry. Histograms were first gated on singlet, live lymphocytes and gating strategies applied to allow phenotyping of cells for expression of CD44, CD45RO and CD62L. The quadrant gate defining expression of CD44 and CD62L (top panel), CD45RO and CD62L (middle panel) or CD44 and CD45RO (bottom panel) was determined using the total live-lymphocyte population, as shown for each surface marker combination in the histograms on the far left of the panel. This quadrant gating strategy was used to determine the surface phenotype of total CD4+IFN-γ+, CD4+IL-2+, CD4+TNF-α+ and triple functional CD4+ cells, as shown in the other histograms. The percentage of cytokine producing CD44hiCD62Llo, CD45RO+CD62Llo and CD44hiCD45RO+ is shown for each of these CD4+ populations. The data is from one representative animal of 5 analysed.
From: Whelan AO, Villarreal-Ramos B, Vordermeier HM, Hogarth PJ (2011)
Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 TEM Cells in Cattle Naturally Infected with Bovine Tuberculosis.
PLoS ONE 6(12): e29194.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Phycoerythrin conjugated Mouse anti Bovine CD335 antibody, clone AKS1 (MCA2365PE) used along with FITC conjugated Mouse anti Bovine CD62L antibody, clone CC32 (MCA1649F) to evaluate NKp46 expression on bovine lymphocyte populations by flow cytometry.
Image caption:
CD62L expression by afferent‐lymph (AL) ‐derived CD2− natural killer (NK) cells may permit homing to lymph nodes (LNs). Lymphocytes from peripheral blood (PB), AL and LNs from seven calves were labelled with monoclonal antibodies for NKp46 and CD62L and analysed by flow cytometry. FACS plots from one representative animal illustrate the expression of NKp46 and CD62L by PB‐, AL‐ and LN‐derived lymphocytes (a). Gates were set based on Fluorescence Minus One controls.
From: Hamilton CA, Mahan S, Bell CR, Villarreal-Ramos B, Charleston B, Entrican G, Hope JC.
Frequency and phenotype of natural killer cells and natural killer cell subsets in bovine lymphoid compartments and blood.
Immunology. 2017 May;151(1):89-97.
doi: 10.1111/imm.12708.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F ResetMouse anti Bovine CD62L:FITC
- Product Type
- Monoclonal Antibody
- Clone
- CC32
- Isotype
- IgG1
- Specificity
- CD62L
Antibody Quality and Performance Guarantee
| Mouse anti Bovine CD62L antibody, clone CC32 recognizes bovine L-selectin, also known as CD62L, Leukocyte-endothelial cell adhesion molecule 1, LECAM-1 or Lymph node homing receptor. Bovine CD62L is a 370 amino acid ~90 kDa, single pass type I transmembrane glycoprotein bearing a single C-type lectin domain, an EGF-like domain and two Sushi domains (UniProt: P98131). Immunoprecipitation of peripheral blood mononuclear cell lysates with Mouse anti Bovine CD62L antibody, clone CC32 reveals a molecule of ~90 kDa when run on polyacrylamide gels under reducing conditions, slightly larger than the murine and human CD62L homologues. Bovine CD62L is expressed on subpopulations of T-lymphocytes expressing CD2, CD4 and CD8. WC1 positive γ/δ T cells also express CD62L as do a subpopulation of WC3+ve B-lymphocytes and all peripheral blood monocytes (Howard et al. 1992). Mouse anti Bovine CD62L antibody, clone CC32 has also been used successfully for the identification of CD62L on ovine peripheral blood cells by flow cytometry (Halliday et al. 2005). |
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Bovine lymphocytes
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NS1 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
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References for CD62L antibody
-
Sopp, P. & Howard, C.J. (2001) IFN gamma and IL-4 production by CD4, CD8 and WC1 gamma delta TCR(+) T cells from cattle lymph nodes and blood.
Vet Immunol Immunopathol. 81 (1-2): 85-96. -
Riollet, C. et al. (2001) Cell subpopulations and cytokine expression in cow milk in response to chronic Staphylococcus aureus infection.
J Dairy Sci. 84: 1077-84. -
Vesosky, B. et al. (2003) Activation marker expression on bovine peripheral blood gammadelta T cells during post-natal development and following vaccination with a commercial polyvalent viral vaccine.
Dev Comp Immunol. 27: 439-47. -
Glew, E.J. et al. (2003) Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells.
J Gen Virol. 84: 1771-80. -
Halliday, S. et al. (2005) Expression of PrPC on cellular components of sheep blood.
J Gen Virol. 86: 1571-9. -
Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitro and in vivo in response to noncytopathic bovine viral diarrhea virus.
J Virol. 79: 7738-44. -
Edwards, J.C. et al. (2010) PrP(Sc) is associated with B cells in the blood of scrapie-infected sheep.
Virology. 405: 110-9. -
Silvestre, F.T. et al. (2011) Effects of differential supplementation of fatty acids during the peripartum and breeding periods of Holstein cows: II. Neutrophil fatty acids and function, and acute phase proteins.
J Dairy Sci. 94: 2285-301.
View The Latest Product References
-
Ozawa, T. et al. (2011) Effect of intramammary infusion of rbGM-CSF on SCC and expression of polymorphonuclear neutrophil adhesion molecules in subclinical mastitis cows.
Vet Res Commun. 36: 21-7. -
Toka, F.N. et al. (2011) Rapid and Transient Activation of {gamma}{delta} T Cells to IFN-{gamma} Production, NK Cell-Like Killing, and Antigen Processing during Acute Virus Infection.
J Immunol. 186: 4853-61. -
Whelan, A.O. et al. (2011) Development of an Antibody to Bovine IL-2 Reveals Multifunctional CD4 T(EM) Cells in Cattle Naturally Infected with Bovine Tuberculosis.
PLoS One. 6: e29194. -
Vrieling M et al. (2012) γδ T cell homing to skin and migration to skin-draining lymph nodes is CCR7 independent.
J Immunol. 188 (2): 578-84. -
Blunt, L. et al. (2015) Phenotypic characterization of bovine memory cells responding to mycobacteria in IFNγ enzyme linked immunospot assays.
Vaccine. 33 (51): 7276-82. -
Hussen, J. et al. (2016) Neutrophil degranulation differentially modulates phenotype and function of bovine monocyte subsets.
Innate Immun. 22 (2): 124-37. -
Chen, X. et al. (2016) Bovine P-selectin mediates leukocyte adhesion and is highly polymorphic in dairy breeds.
Res Vet Sci. 108: 85-92. -
Jimbo, S. et al. (2017) Effect of Mycoplasma bovis on bovine neutrophils.
Vet Immunol Immunopathol. 188: 27-33. -
Hamilton, C.A. et al. (2017) Frequency and phenotype of natural killer cells and natural killer cell subsets in bovine lymphoid compartments and blood.
Immunology. 151 (1): 89-97. -
Souza, F.N. et al. (2020) Immune response in nonspecific mastitis: What can it tell us?
J Dairy Sci. 103 (6): 5376-86. -
Fiorenza, M.F. et al. (2021) Neutrophils recognize and amplify IFNT signals derived from day 7 bovine embryo for stimulation of ISGs expression in vitro.: A possible implication for the early maternal recognition of pregnancy.
Biochem Biophys Res Commun. 553: 37-43. -
Martin, C.C. et al. (2021) Effect of prophylactic use of tulathromycin on gut bacterial populations, inflammatory profile and diarrhea in newborn Holstein calves.
Res Vet Sci. 136: 268-76. -
Hong, S. et al. (2024) Impact of an Injectable Trace Mineral Supplement on the Immune Response and Outcome of Mannheimia haemolytica Infection in Feedlot Cattle.
Biol Trace Elem Res. Jun 10 [Epub ahead of print].
- Synonyms
- LECAM-1
- L-SELECTIN
- RRID
- AB_905968
- UniProt
- P98131
- Entrez Gene
- SELL
- GO Terms
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0005529 sugar binding
MCA1649F
1606
1808
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