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G-CSF antibody | BVD11-37G10

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Sandwich ELISA analysis of Human G-CSF using Rat anti human G-CSF (1012701) as a capture reagent and biotinylated Rat anti Human G-CSF antibody, clone BVD11-37G10 (1012808) as a detection reagent with recombinant human G-CSF (PHP082) as target antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Human serum (orange) and plasma (green) samples are tested undiluted

Rat anti Human G-CSF:Biotin

Product Type
Monoclonal Antibody
Clone
BVD11-37G10
Isotype
IgG2a
Specificity
G-CSF

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Product Code Applications Pack Size List Price Your Price Qty
1012808
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.5 mg loader
List Price Your Price
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Rat anti Human G-CSF antibody, clone BVD11-37G10 recognizes human granulocyte colony stimulating factor (G-CSF), also known as Pluripoietin. In addition Filgrastim and Lenograstim are used to describe recombinant human G-CSF preparations. Human G-CSF is a 207 amino acid, ~20 kDa O-linked glycoprotein belonging to the IL-6 superfamily. There are potentially three isoforms formed by alternative splicing, Rat anti Human G-CSF antibody, clone BVD13-3A5 binds the canonical isoform-1, binding to other isoforms has not been assessed (UniProt: P09919).

G-CSF is produced by activated macrophages, monocytes and neutrophils. In addition it can be produced by fibroblasts and endothelial cells (Zsebo et al. 1988). G-CSF stimulates production of cells belonging to the granulocytic lineage in the bone marrow and is an activating agent for mature neutrophilic granulocytes (Scapini et al. 2003) to produce other cytokines including CD257 (BAFF), operating through binding to the G-CSF receptor, CD114.

Biotinylated Rat anti Human G-CSF antibody, clone BVD11-37G10 has been successfully evaluated as a detection reagent in a highly sensitive sandwich ELISA assay with biotinylated Rat anti Human G-CSF, clone BVD11-3A5 (1012701) as a capture reagent and recombinant human G-CSF (PHP082B) as antigen.

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid.
Preparation
Antibody purified from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Approx. Protein Concentrations
0.5 mg/ml
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with 1012701 as the capture reagent.

RRID
AB_2083736
UniProt
P09919
Entrez Gene
CSF3
GO Terms
GO:0005125 cytokine activity
GO:0005130 granulocyte colony-stimulating factor receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0008284 positive regulation of cell proliferation
GO:0019899 enzyme binding
GO:0019221 cytokine-mediated signaling pathway
GO:0030851 granulocyte differentiation

1012808

153365 153672

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