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G-CSF antibody | BVD13-3A5

Rat anti Human G-CSF

Product Type
Monoclonal Antibody
Clone
BVD13-3A5
Isotype
IgG1
Specificity
G-CSF

Product Code Applications Pack Size List Price Your Price Qty
1012701
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.5 mg loader
List Price Your Price
loader

Rat anti Human G-CSF antibody, clone BVD13-3A5 recognizes human granulocyte colony stimulating factor (G-CSF), also known as Pluripoietin. In addition Filgrastim and Lenograstim are used to describe recombinant human G-CSF preparations. Human G-CSF is a 207 amino acid, ~20 kDa O-linked glycoprotein belonging to the IL-6 superfamily. There are potentially three isoforms formed by alternative splicing, Rat anti Human G-CSF antibody, clone BVD13-3A5 binds the canonical isoform-1, binding to other isoforms has not been assessed (UniProt: P09919).

G-CSF is produced by activated macrophages, monocytes and neutrophils. In addition it can be produced by fibroblasts and endothelial cells (Zsebo et al. 1988). G-CSF stimulates production of cells belonging to the granulocytic lineage in the bone marrow and is an activating agent for mature neutrophilic granulocytes (Scapini et al. 2003) to produce other cytokines including CD257 (BAFF), operating through binding to the G-CSF receptor CD114.

Rat anti Human G-CSF antibody, clone BVD13-3A5 has been successfully evaluated as a capture reagent in a highly sensitive sandwich ELISA assay with biotinylated Rat anti Human G-CSF, clone BVD11-37G10 (1012808) as a detection reagent and recombinant human G-CSF (PHP082B) as antigen.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Antibody purified from ascites
Buffer Solution
Borate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant human granulocyte-colony stimulating factor expressed in E. coil.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting 1ug/ml 5ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with 1012808 as the detection reagent.

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Description Rabbit F(ab')2 anti Rat IgG:FITC
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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
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Description Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed)
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) STAR71D650 F IF 0.1 mg loader
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
List Price Your Price
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
List Price Your Price
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

RRID
AB_620005
UniProt
P09919
Entrez Gene
CSF3
GO Terms
GO:0005125 cytokine activity
GO:0005130 granulocyte colony-stimulating factor receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0008284 positive regulation of cell proliferation
GO:0019899 enzyme binding
GO:0019221 cytokine-mediated signaling pathway
GO:0030851 granulocyte differentiation

1012701

153457 155048 158929 162704

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