Herpes simplex Virus 1/2 antibody
Rabbit anti Herpes simplex virus antibody (4956-0404) used for the identification oand distribution of HSV in infected mouse brains by immunofluorescence on free floating tissue sections.
Image caption:
Impact of CCR2 deficiency in resident cells of the CNS or in the hematopoietic system on HSV-1 distribution in the brain during HSE.
Representative micrographs illustrating the distribution pattern of HSV-1 proteins in the olfactory bulb, the medulla, the cerebral cortex and the striatum of wild-type (WT) C57BL/6, CCR2-/-→WT, WT→CCR2-/- and CCR2-/- animals (4 to 5 mice per group) sacrificed on day 6 post-infection. Brain slices (25 μm thick) were processed for immunohistochemistry analyses with a polyclonal rabbit anti-HSV-1/2 primary antibody and an Alexa 594-conjugated chicken anti-rabbit secondary antibody (red), followed by nuclear staining with DAPI (blue). Scale bar 100 μm.
From: Menasria R, Canivet C, Piret J, Gosselin J, Boivin G (2016)
Both Cerebral and Hematopoietic Deficiencies in CCR2 Result in Uncontrolled Herpes Simplex Virus Infection of the Central Nervous System in Mice.
PLoS ONE 11(12): e0168034.
Rabbit anti Herpes simplex virus antibody recognizes Herpes Simplex viruses 1 & 2 and reacts with ICPs and late structural (virion) antigens. It does not react with uninfected HEp-2 cells.
Product Details
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide (NaN3) - Immunogen
- Human HSV, strain F.
- Approx. Protein Concentrations
- Ig concentration 4.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of Herpes simplex Virus 1/2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Product Specific References
References for Herpes simplex Virus 1/2 antibody
-
Boivin, N. et al. (2012) Impact of deficiency in CCR2 and CX3CR1 receptors on monocytes trafficking in herpes simplex virus encephalitis.
J Gen Virol. 93: 1294-304. -
Menasria, R. et al. (2013) Both TRIF and IPS-1 Adaptor Proteins Contribute to the Cerebral Innate Immune Response against Herpes Simplex Virus 1 Infection.
J Virol. 87: 7301-8. -
Menasria, R. et al. (2016) Both Cerebral and Hematopoietic Deficiencies in CCR2 Result in Uncontrolled Herpes Simplex Virus Infection of the Central Nervous System in Mice.
PLoS One. 11 (12): e0168034.
