Herpes simplex Virus 1/2 antibody
Rabbit anti Herpes simplex virus antibody (4956-0404) used for the identification oand distribution of HSV in infected mouse brains by immunofluorescence on free floating tissue sections.
Image caption:
Impact of CCR2 deficiency in resident cells of the CNS or in the hematopoietic system on HSV-1 distribution in the brain during HSE.
Representative micrographs illustrating the distribution pattern of HSV-1 proteins in the olfactory bulb, the medulla, the cerebral cortex and the striatum of wild-type (WT) C57BL/6, CCR2-/-→WT, WT→CCR2-/- and CCR2-/- animals (4 to 5 mice per group) sacrificed on day 6 post-infection. Brain slices (25 μm thick) were processed for immunohistochemistry analyses with a polyclonal rabbit anti-HSV-1/2 primary antibody and an Alexa 594-conjugated chicken anti-rabbit secondary antibody (red), followed by nuclear staining with DAPI (blue). Scale bar 100 μm.
From: Menasria R, Canivet C, Piret J, Gosselin J, Boivin G (2016)
Both Cerebral and Hematopoietic Deficiencies in CCR2 Result in Uncontrolled Herpes Simplex Virus Infection of the Central Nervous System in Mice.
PLoS ONE 11(12): e0168034.
Rabbit anti Herpes simplex virus antibody recognizes Herpes Simplex viruses 1 & 2 and reacts with ICPs and late structural (virion) antigens. It does not react with uninfected HEp-2 cells.
Product Details
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide (NaN3) - Immunogen
- Human HSV, strain F.
- Approx. Protein Concentrations
- Ig concentration 4.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Herpes simplex Virus 1/2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Product Specific References
References for Herpes simplex Virus 1/2 antibody
-
Boivin, N. et al. (2012) Impact of deficiency in CCR2 and CX3CR1 receptors on monocytes trafficking in herpes simplex virus encephalitis.
J Gen Virol. 93: 1294-304. -
Menasria, R. et al. (2013) Both TRIF and IPS-1 Adaptor Proteins Contribute to the Cerebral Innate Immune Response against Herpes Simplex Virus 1 Infection.
J Virol. 87: 7301-8. -
Menasria, R. et al. (2016) Both Cerebral and Hematopoietic Deficiencies in CCR2 Result in Uncontrolled Herpes Simplex Virus Infection of the Central Nervous System in Mice.
PLoS One. 11 (12): e0168034.
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