Hepatitis B Surface Antigen antibody

Rabbit anti Hepatitis B Surface Antigen

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Hepatitis B Surface Antigen

Product Code Applications Pack Size List Price Your Price Qty
OBT0990
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Rabbit anti hepatitis B surface antigen antibody recognizes Hepatitis B surface antigen, recognizing subtypes ad and ay. Hepatitis B virus is a major causative agent of acute and chronic liver disease in humans.

Hepatitis B surface antigen (HBsAg) is a protein component of the viral envelope, which is expressed predominantly in the cytoplasm of infected hepatocytes.

Target Species
Viral
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Hepatitis B were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Hepatitis B subtypes ad and ay.
Approx. Protein Concentrations
IgG concentration 4.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/10,000
Immunohistology - Frozen
Immunohistology - Paraffin
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for Hepatitis B Surface Antigen antibody

  1. Deng, L. et al. (2009) Hepatitis B virus inhibition in mice by lentiviral vector mediated short hairpin RNA.
    BMC Gastroenterol. 9: 73.
  2. Ying, R.S. et al. (2007) Hepatitis B virus is inhibited by RNA interference in cell culture and in mice.
    Antiviral Res. 73 (1): 24-30.
  3. Donate, A. et al (2011) Evaluation of a Novel Non-Penetrating Electrode for Use in DNA Vaccination
    PLoS One. 6: e19181.
  4. Harahap-carrillo, I.S. et al. (2015) Immunogenic Subviral Particles Displaying Domain III of Dengue 2 Envelope Protein Vectored by Measles Virus.
    Vaccines (Basel). 3 (3): 503-18.

Immunohistology - Frozen

Synonyms
HBsAg
RRID
AB_609657
View more products with HEPATITIS B specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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