Hepatitis B Surface Antigen antibody
Rabbit anti hepatitis B surface antigen antibody (OBT0990) used for the evaluation of hepatitis B surface antigen in infected cell cultures by western blotting
Image caption:
(A) Materials released from Vero/hSLAM cells infected with MVvac2DIII-S,S)P or MVvac2(HBsAg)N collected 72 h after infection were clarified, and particulate material pelleted, loaded on a 20% to 60% sucrose gradient, and centrifuged to equilibrium. Five hundred microliter fractions were collected from the top (left) to the bottom (right) and weighed. The dotted line shows a representative density profile. (B) Aliquots of each fraction were separated by 12.5% SDS-polyacrylamide gel electrophoresis, immunoblotted, and probed with anti-HBsAg antibodies for fractions coming from cells infected with MVvac2DIII-S,S)P (upper panel) or MVvac2(HBsAg)N (medium panel). The lower panel indicates the migration of infectious MV virions.
From: Immunogenic Subviral Particles Displaying Domain III of Dengue 2 Envelope Protein Vectored by Measles Virus.
Harahap-Carrillo, I.S. et al.
Vaccines 2015; 3(3), 503-18. doi:10.3390/vaccines3030503.
Rabbit anti hepatitis B surface antigen antibody (OBT0990) used for the detection of hepatitis b surface antigen in infected mouse liver by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical staining for HBsAg (A-D) and HBcAg (E-H) in liver sections. Treatment with pWPT-HBV-shRNA-1 lentivirus(C, G) or pWPT-HBV-shRNA-2 lentivirus (D, H) reduces the number of HBsAg or HBcAg-positive cells on day 7 respectively compared to the control. The staining was performed on tissue sections from three animals per group. Representative sections are shown (four sections per animals were stained and counted). Numerous HBsAg-positive and HBcAg-positive cells were seen in the empty vector group (A, E) and negative control RNAi group (B, F). No HBsAg-positive stained cells were observed in most fields in pWPT-HBV-shRNA-2 group(D). Some stained cells could be seen in pWPT-HBV-shRNA-1 or shRNA-2 groups(C, G, H) and these were indicated by arrows, but staining were much less intense than that in control groups (100× original magnification, scale bar 200 μm).
From: Deng L, Li G, Xi L, Yin A, Gao Y, You W, Wang X, Sun B. Hepatitis B virus inhibition in mice by lentiviral vector mediated short hairpin RNA.
BMC Gastroenterol. 2009 Oct 6;9:73.
Rabbit anti hepatitis B surface antigen antibody (OBT0990) used for the detection of Hepatitis s antigen in guinea pig skin by immunohistochemistry on cryosections.
Image caption:
Plasmid expression and inflammation in the skin. Guinea pigs were treated with pHBsAg. Expression of plasmid was evaluated at 48 hrs post treatment by IHC (A-IO; B I+EP). Inflammation was measured 96 hrs post treatment and assessed by H&E (C-No treatment; D- IO, E–I+EP) at 100× magnification and 200× magnification (F- I+EP).
From: Donate A, Coppola D, Cruz Y, Heller R (2011) Evaluation of a Novel Non-Penetrating Electrode for Use in DNA Vaccination.
PLoS ONE 6(4): e19181.
Rabbit anti hepatitis B surface antigen antibody recognizes Hepatitis B surface antigen, recognizing subtypes ad and ay. Hepatitis B virus is a major causative agent of acute and chronic liver disease in humans.
Hepatitis B surface antigen (HBsAg) is a protein component of the viral envelope, which is expressed predominantly in the cytoplasm of infected hepatocytes.
Hepatitis B surface antigen (HBsAg) is a protein component of the viral envelope, which is expressed predominantly in the cytoplasm of infected hepatocytes.
Product Details
- Target Species
- Viral
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to Hepatitis B were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.002% Thiomersal
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Hepatitis B subtypes ad and ay.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Approx. Protein Concentrations
- IgG concentration 4.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch.
- Guarantee
- 12 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of Hepatitis B Surface Antigen antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/200 | 1/1000 | |
| ELISA | 1/10,000 | ||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- ELISA
- This product may be used as a detection reagent in a sandwich ELISA together with MCA4658 as the capture reagent.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | C E P WB | 1 ml |
|
|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
|
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml |
Product Specific References
References for Hepatitis B Surface Antigen antibody
-
Deng, L. et al. (2009) Hepatitis B virus inhibition in mice by lentiviral vector mediated short hairpin RNA.
BMC Gastroenterol. 9: 73. -
Ying, R.S. et al. (2007) Hepatitis B virus is inhibited by RNA interference in cell culture and in mice.
Antiviral Res. 73 (1): 24-30. -
Donate, A. et al (2011) Evaluation of a Novel Non-Penetrating Electrode for Use in DNA Vaccination
PLoS One. 6: e19181. -
Harahap-carrillo, I.S. et al. (2015) Immunogenic Subviral Particles Displaying Domain III of Dengue 2 Envelope Protein Vectored by Measles Virus.
Vaccines (Basel). 3 (3): 503-18.
