Hepatitis B Surface Antigen antibody
Rabbit anti hepatitis B surface antigen antibody (OBT0990) used for the evaluation of hepatitis B surface antigen in infected cell cultures by western blotting.
Image caption:
(A) Materials released from Vero/hSLAM cells infected with MVvac2DIII-S,S)P or MVvac2(HBsAg)N collected 72 h after infection were clarified, and particulate material pelleted, loaded on a 20% to 60% sucrose gradient, and centrifuged to equilibrium. Five hundred microliter fractions were collected from the top (left) to the bottom (right) and weighed. The dotted line shows a representative density profile. (B) Aliquots of each fraction were separated by 12.5% SDS-polyacrylamide gel electrophoresis, immunoblotted, and probed with anti-HBsAg antibodies for fractions coming from cells infected with MVvac2DIII-S,S)P (upper panel) or MVvac2(HBsAg)N (medium panel). The lower panel indicates the migration of infectious MV virions.
From: Immunogenic Subviral Particles Displaying Domain III of Dengue 2 Envelope Protein Vectored by Measles Virus.
Harahap-Carrillo, I.S. et al.
Vaccines 2015; 3(3), 503-18. doi:10.3390/vaccines3030503.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti hepatitis B surface antigen antibody (OBT0990) used for the detection of hepatitis b surface antigen in infected mouse liver by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical staining for HBsAg (A-D) and HBcAg (E-H) in liver sections. Treatment with pWPT-HBV-shRNA-1 lentivirus(C, G) or pWPT-HBV-shRNA-2 lentivirus (D, H) reduces the number of HBsAg or HBcAg-positive cells on day 7 respectively compared to the control. The staining was performed on tissue sections from three animals per group. Representative sections are shown (four sections per animals were stained and counted). Numerous HBsAg-positive and HBcAg-positive cells were seen in the empty vector group (A, E) and negative control RNAi group (B, F). No HBsAg-positive stained cells were observed in most fields in pWPT-HBV-shRNA-2 group(D). Some stained cells could be seen in pWPT-HBV-shRNA-1 or shRNA-2 groups(C, G, H) and these were indicated by arrows, but staining were much less intense than that in control groups (100× original magnification, scale bar 200 μm).
From: Deng L, Li G, Xi L, Yin A, Gao Y, You W, Wang X, Sun B.
Hepatitis B virus inhibition in mice by lentiviral vector mediated short hairpin RNA.
BMC Gastroenterol. 2009 Oct 6;9:73.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti hepatitis B surface antigen antibody (OBT0990) used for the detection of Hepatitis s antigen in guinea pig skin by immunohistochemistry on cryosections.
Image caption:
Plasmid expression and inflammation in the skin. Guinea pigs were treated with pHBsAg. Expression of plasmid was evaluated at 48 hrs post treatment by IHC (A-IO; B I+EP). Inflammation was measured 96 hrs post treatment and assessed by H&E (C-No treatment; D- IO, E–I+EP) at 100× magnification and 200× magnification (F- I+EP).
From: Donate A, Coppola D, Cruz Y, Heller R (2011)
Evaluation of a Novel Non-Penetrating Electrode for Use in DNA Vaccination.
PLoS ONE 6(4): e19181.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Hepatitis B Surface Antigen
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Hepatitis B Surface Antigen
Antibody Quality and Performance Guarantee
| Rabbit anti hepatitis B surface antigen antibody recognizes Hepatitis B surface antigen, recognizing subtypes ad and ay. Hepatitis B virus is a major causative agent of acute and chronic liver disease in humans. Hepatitis B surface antigen (HBsAg) is a protein component of the viral envelope, which is expressed predominantly in the cytoplasm of infected hepatocytes. |
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to Hepatitis B were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Hepatitis B subtypes ad and ay.
- Approx. Protein Concentrations
- IgG concentration 5.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/10,000 | |
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
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| Description | Antigen Retrieval Buffer, pH8.0 | ||||||
References for Hepatitis B Surface Antigen antibody
-
Deng, L. et al. (2009) Hepatitis B virus inhibition in mice by lentiviral vector mediated short hairpin RNA.
BMC Gastroenterol. 9: 73. -
Ying, R.S. et al. (2007) Hepatitis B virus is inhibited by RNA interference in cell culture and in mice.
Antiviral Res. 73 (1): 24-30. -
Donate, A. et al (2011) Evaluation of a Novel Non-Penetrating Electrode for Use in DNA Vaccination
PLoS One. 6: e19181. -
Harahap-carrillo, I.S. et al. (2015) Immunogenic Subviral Particles Displaying Domain III of Dengue 2 Envelope Protein Vectored by Measles Virus.
Vaccines (Basel). 3 (3): 503-18.
- Synonyms
- HBsAg
- RRID
- AB_609657
View more products with HEPATITIS B specificity
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