Histone H4 Ac12 antibody

Rabbit anti Histone H4 (Ac12)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Histone H4 Ac12

Product Code Applications Pack Size List Price Your Price Qty
AHP2258
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SDS Safety Datasheet SDS
E WB 0.1 ml loader
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Rabbit anti Histone H4 (Ac12) antibody recognizes Histone H4 acetylated at lysine 12. Histone H4 is a 102 amino acid ~12 kDa nuclear transcritional regulator involved in DNA repair, replication and chromosome stability.

Target Species
Synthetic Peptide
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Drosophila
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to acetylated histone H4 were raised by repeated immunisation of rabbits with highly purified antigen.
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting 1/500
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for Histone H4 Ac12 antibody

  1. Turner, B.M. et al. (1989) Histone H4 acetylation in human cells. Frequency of acetylation at different sites defined by immunolabeling with site-specific antibodies.
    FEBS Lett. 253 (1-2): 141-5.
  2. Turner, B.M. et al. (1992) Histone H4 isoforms acetylated at specific lysine residues define individual chromosomes and chromatin domains in Drosophila polytene nuclei.
    Cell. 69 (2): 375-84.
  3. Belyaev, N. et al. (1996) Differential underacetylation of histones H2A, H3 and H4 on the inactive X chromosome in human female cells.
    Hum Genet. 97 (5): 573-8.
  4. Tsaprouni, L.G. et al. (2011) Differential patterns of histone acetylation in inflammatory bowel diseases.
    J Inflamm (Lond). 8 (1): 1.
  5. Chen, W.Y. et al. (2000) Molecular mechanism for silencing virally transduced genes involves histone deacetylation and chromatin condensation.
    Proc Natl Acad Sci U SA. 97: 377-82.
  6. Friis, R.M. et al. (2009) A glycolytic burst drives glucose induction of global histone acetylation by picNuA4 and SAGA.
    Nucleic Acids Res. 37: 3969-80.
  7. Ito, K. et al. (2000) Glucocorticoid receptor recruitment of histone deacetylase 2 inhibits interleukin-1beta-induced histone H4 acetylation on lysines 8 and 12.
    Mol Cell Biol. 20: 6891-903.
  8. Kobor, M.S. et al (2004) A protein complex containing the conserved Swi2/Snf2-related ATPase Swr1p deposits histone variant H2A.Z into euchromatin.
    PLoS Biol. 2: E131.
  9. View The Latest Product References
  10. Lin, X. et al. (2001) Reversal of GSTP1 CpG island hypermethylation and reactivation of pi-class glutathione S-transferase (GSTP1) expression in human prostate cancer cells by treatment with procainamide.
    Cancer Res. 61: 8611-6.
  11. Nie, M. et al. (2003) Transcriptional regulation of cyclooxygenase 2 by bradykinin and interleukin-1beta in human airway smooth muscle cells: involvement of different promoter elements, transcription factors, and histone h4 acetylation.
    Mol Cell Biol. 23: 9233-44.
  12. Tsaprouni, L.G. et al. (2011) Differential patterns of histone acetylation in inflammatory bowel diseases.
    J Inflamm (Lond). 8: 1.
  13. Tabuchi, T.M. et al. (2011) Chromosome-biased binding and gene regulation by the Caenorhabditis elegans DRM complex.
    PLoS Genet. 7 (5): e1002074.

Chromatin Immunoprecipitation

Immunofluorescence

Western Blotting

RRID
AB_10897654
UniProt
P62805
Entrez Gene
HIST2H4B
GO Terms
GO:0005515 protein binding
GO:0000723 telomere maintenance
GO:0000786 nucleosome
GO:0003677 DNA binding
GO:0005654 nucleoplasm
GO:0048015 phosphatidylinositol-mediated signaling
GO:0034080 CenH3-containing nucleosome assembly at centromere
GO:0045653 negative regulation of megakaryocyte differentiation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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