TNF Alpha antibody

Rabbit anti Sheep TNF Alpha

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
TNF Alpha

Product Code Applications Pack Size List Price Your Price Qty
AHP2815
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Rabbit anti Sheep TNF alpha recognizes sheep tumor necrosis factor (TNF) alpha, a 25.5 kDa regulatory cytokine.

TNF alpha is produced by activated macrophages, monocytes, endothelial cells, fibroblast and both T and B lymphocytes. Production of ovine TNF alpha can be induced by a variety of factors, dependant upon cell type, and includes bacterial toxins, IL-1, PDGF, IFN beta, NGF, oncostatin M and viral infections. TNF alpha functions in the regulation of immune cells, is able to induce fever, apoptotic cell death, sepsis, cacheria, inflammation and to inhibit tumorigenesis and viral replication.

Target Species
Sheep
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to sheep TNF alpha was raised by repeated immunization of rabbit with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant ovine TNF ALPHA
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml 5.0 ug/ml
Western Blotting 0.1 ug/ml 2.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This product may be used as a capture antibody in a sandwich ELISA together with AHP2815B as the detection reagent. PSP005 may be used as a standard.

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Description Goat anti Rabbit IgG (Fc):Biotin
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
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Description Goat anti Rabbit IgG (Fc):FITC
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
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Description Goat anti Rabbit IgG (Fc):HRP
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
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Description Goat anti Rabbit IgG (H/L):HRP
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
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Description Sheep anti Rabbit IgG:FITC
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
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Description Sheep anti Rabbit IgG:RPE

UniProt
P23383
Entrez Gene
TNF
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
View more products with TNF ALPHA specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Sheep Products