Tyrosine Hydroxylase antibody
Rabbit anti tyrosine hydroxylase antibody (AHP931) used to demonstrate tyrosine hydroxylase reactivity in nerve fibers in a rat myocarcial ischemia model by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
TH immunohistochemical staining of the infarct border zone (DAB color, ×200). (a) Sham-operated group. The distribution of TH-positive nerve fibers is orderly. (b) Control ischemic group. TH-positive nerve fibers in ventricular muscle increased significantly it is thickened and disorderly. (c) Preischemic treatment group. (d) Delayed application group. The distribution of nerve fibers after treatment with desipramine significantly improved. The change of preischemic treatment group was more obvious.
From: Wu X, Jiang H, Yu L, Hu X, Liu W.
Desipramine pretreatment improves sympathetic remodeling and ventricular fibrillation threshold after myocardial ischemia.
J Biomed Biotechnol. 2012;2012:732909.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rabbit anti Tyrosine Hydroxylase
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Tyrosine Hydroxylase
Antibody Quality and Performance Guarantee
| Rabbit anti Rat tyrosine hydroxylase antibody recognizes tyrosine hydroxylase (TH).
Tyrosine hydroxylase catalyses the rate-limiting step in the biosynthetic pathway of the catecholamines dopamine (DA), norepinephrine, and epinephrine. The enzyme exists as a tetramer, with each subunit composed of an N-terminal regulatory domain and a C-terminal catalytic domain. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson's disease (Haavik et al. 1998). |
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mammals Expected from Sequence Grasshopper (Gastrimargus musicus) Rat Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to tyrosine hydroxylase were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- 10mM HEPES pH7.5
- Preservative Stabilisers
0.09% Sodium Azide 0.01% Bovine Serum Albumin 50% Glycerol - Immunogen
- Tyrosine hydroxylase purified from pheochromocytoma.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
1/1000 | |
| Immunohistology - Frozen | |
1/1000 | |
| Immunohistology - Paraffin | |
||
| Western Blotting | |
1/1000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP931 detects a band of approximately 60kDa in rat caudate cell lysates.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | Antigen Retrieval Buffer, pH8.0 | ||||||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
References for Tyrosine Hydroxylase antibody
-
Xu, Z.Q. et al. (1998) Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site- and phosphorylation state- specific antibodies.
Neurosci. 82: 727-8. -
Kim, S.M. et al. (2009) Dense-core vesicle proteins IA-2 and IA-2{beta} affect renin synthesis and secretion through the {beta}-adrenergic pathway.
Am J Physiol Renal Physiol. 296: F382-9. -
Fan, W. et al. (2010) Structural and cellular features in metaphyseal and diaphyseal periosteum of osteoporotic rats.
J Mol Histol. 41: 51-60. -
Wu, X. et al. (2012) Desipramine pretreatment improves sympathetic remodeling and ventricular fibrillation threshold after myocardial ischemia.
J Biomed Biotechnol. 2012: 732909. -
Nurul Wahida, A. & Cooper, P.D. (2014) Feeding and the salivary gland response in free-ranging yellow-winged grasshoppers (Gastrimargus musicus)
Austral J Zool. 62: 393-400.
- RRID
- AB_2201650
- UniProt
- P04177
- Entrez Gene
- Th
- GO Terms
- GO:0042136 neurotransmitter biosynthetic process
- GO:0001666 response to hypoxia
- GO:0005739 mitochondrion
- GO:0004511 tyrosine 3-monooxygenase activity
- GO:0005829 cytosol
- GO:0055114 oxidation-reduction process
- GO:0006585 dopamine biosynthetic process from tyrosine
- GO:0008021 synaptic vesicle
- GO:0008198 ferrous iron binding
- View More GO Terms
- GO:0008199 ferric iron binding
- GO:0019825 oxygen binding
- GO:0016597 amino acid binding
- GO:0019717 synaptosome
- GO:0034617 tetrahydrobiopterin binding
- GO:0035240 dopamine binding
- GO:0043195 terminal button
- GO:0048545 response to steroid hormone stimulus
- GO:0051289 protein homotetramerization
AHP931
148677
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