Tyrosine Hydroxylase antibody

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Rabbit anti Tyrosine Hydroxylase

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP931 C IF P WB 0.1 ml
Rabbit anti Rat tyrosine hydroxylase antibody recognizes tyrosine hydroxylase (TH). Tyrosine hydroxylase catalyses the rate-limiting step in the biosynthetic pathway of the catecholamines dopamine (DA), norepinephrine, and epinephrine. The enzyme exists as a tetramer, with each subunit composed of an N-terminal regulatory domain and a C-terminal catalytic domain.

Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson's disease (Haavik et al. 1998).

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mammals Expected from Sequence
Grasshopper (Gastrimargus musicus)
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to tyrosine hydroxylase were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
10mM HEPES pH7.5
Preservative Stabilisers
0.05%Sodium Azide
0.01%Bovine Serum Albumin
50%Glycerol
Immunogen
Tyrosine hydroxylase purified from pheochromocytoma.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
P04177 Related reagents
Entrez Gene
Th Related reagents
GO Terms
GO:0042136 neurotransmitter biosynthetic process
GO:0001666 response to hypoxia
GO:0005739 mitochondrion
GO:0004511 tyrosine 3-monooxygenase activity
GO:0005829 cytosol
GO:0055114 oxidation-reduction process
GO:0006585 dopamine biosynthetic process from tyrosine
GO:0008021 synaptic vesicle
GO:0008198 ferrous iron binding
GO:0008199 ferric iron binding
GO:0019825 oxygen binding
GO:0016597 amino acid binding
GO:0019717 synaptosome
GO:0034617 tetrahydrobiopterin binding
GO:0035240 dopamine binding
GO:0043195 terminal button
GO:0048545 response to steroid hormone stimulus
GO:0051289 protein homotetramerization
Regulatory
For research purposes only

Applications of Tyrosine Hydroxylase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/1000
Immunohistology - Frozen 1/1000
Immunohistology - Paraffin
Western Blotting 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP931 detects a band of approximately 60kDa in rat caudate cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Application Based External Images

Immunofluorescence

Immunohistology - Paraffin

Product Specific References

References for Tyrosine Hydroxylase antibody

  1. Xu, Z.Q. et al. (1998) Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site- and phosphorylation state- specific antibodies.
    Neurosci. 82: 727-8.
  2. Fan, W. et al. (2010) Structural and cellular features in metaphyseal and diaphyseal periosteum of osteoporotic rats.
    J Mol Histol. 41: 51-60.
  3. Kim, S.M. et al. (2009) Dense-core vesicle proteins IA-2 and IA-2{beta} affect renin synthesis and secretion through the {beta}-adrenergic pathway.
    Am J Physiol Renal Physiol. 296: F382-9.
  4. Nurul Wahida, A. & Cooper, P.D. (2014) Feeding and the salivary gland response in free-ranging yellow-winged grasshoppers (Gastrimargus musicus)
    Austral J Zool. 62: 393-400.
  5. Wu, X. et al. (2012) Desipramine pretreatment improves sympathetic remodeling and ventricular fibrillation threshold after myocardial ischemia.
    J Biomed Biotechnol. 2012: 732909.