MCP-1 antibody

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Anti Rat MCP-1 Antibody thumbnail image 2

Anti Rat MCP-1 Antibody gallery image 1 — **Published customer image:**
Rabbit anti Rat MCP-1 antibody (**AAR31**) used for the detection of MCP1 in rat temporoparietal cortex homogenates by ELISA.
**Image caption:**
The administration of the combined treatment with zinc and selenium modifies the protein levels of CCL2 in the rat hypoxia-ischemia model.CCAO: common carotid artery occlusion for 10 min; Zn + CCAO: chronic zinc administration before CCAO. Zn + CCAO + Se: chronic zinc administration before CCAO followed by selenium administration. The values were normalized against the control group. nv: normalized values. Each value represents of 5 independent experiments made in triplicate. , one-way ANOVA with post hoc Dunnetts test when compared with the untreated control group; , Student's -test when compared with the respective CCAO group.

From: Tomas-Sanchez C, *et al*.
Prophylactic Zinc and Therapeutic Selenium Administration Increases the Antioxidant Enzyme Activity in the Rat Temporoparietal Cortex and Improves Memory after a Transient Hypoxia-Ischemia.
Oxid Med Cell Longev. 2018:9416432.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Anti Rat MCP-1 Antibody gallery image 2 — **Published customer image:**
Rabbit anti Rat MCP-1 antibody (**AAR31**) used to assess CCL2 expression in rat spinal cord tissue by immunofluorescence and western blotting.
**Image caption:**
After traumatic SCI, astrocytes release more sEVs that encapsulate CCL2. a, b Western blotting to detect the expression of CCL2 in spinal cord tissue. c qRT-PCR to detect the expression of CCL2 in spinal cord tissue. d Immunofluorescence co-localization of astrocyte markers GFAP and CCL2.

From: Rong Y, Ji C, Wang Z, Ge X, Wang J, Ye W, Tang P, Jiang D, Fan J, Yin G, Liu W, Cai W.
Small extracellular vesicles encapsulating CCL2 from activated astrocytes induce microglial activation and neuronal apoptosis after traumatic spinal cord injury.
J Neuroinflammation. 2021 Sep 12;18(1):196.
doi: 10.1186/s12974-021-02268-y.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Rabbit anti Rat MCP-1

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
AAR31 Datasheet	C E FN WB SDS	0.1 mg	Log in		Get Quote

Rabbit anti Rat MCP-1 antibodyrecognizes rat monocyte chemo attractant protein-1 (MCP-1), also known as CCL-2.

Rat MCP-1 is a chemokine of approximately 12 kDa, which plays an important role in the inflammatory response of blood monocytes and tissue macrophages.

Product Details

Target Species: Rat
Product Form: Purified IgG - lyophilised
Reconstitution: Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation: Antisera to rat MCP-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: None present
Carrier Free: Yes
Immunogen: Recombinant rat MCP-1.
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml after reconstitution.

Storage Information

Storage: Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 12 months from date of despatch

More Information

UniProt: P14844
Entrez Gene: Ccl2
GO Terms: GO:0001666 response to hypoxia; GO:0001938 positive regulation of endothelial cell proliferation; GO:0005615 extracellular space; GO:0005737 cytoplasm; GO:0006874 cellular calcium ion homeostasis; GO:0009612 response to mechanical stimulus; GO:0006954 inflammatory response; GO:0006955 immune response; GO:0007179 transforming growth factor beta receptor signaling pathway; GO:0007568 aging; GO:0008009 chemokine activity; GO:0008201 heparin binding; GO:0042493 response to drug; GO:0009408 response to heat; GO:0010332 response to gamma radiation; GO:0014823 response to activity; GO:0046677 response to antibiotic; GO:0030593 neutrophil chemotaxis; GO:0031100 organ regeneration; GO:0031727 CCR2 chemokine receptor binding; GO:0032496 response to lipopolysaccharide; GO:0032570 response to progesterone stimulus; GO:0033552 response to vitamin B3; GO:0042466 chemokinesis; GO:0043025 neuronal cell body; GO:0043200 response to amino acid stimulus; GO:0045471 response to ethanol; GO:0048010 vascular endothelial growth factor receptor signaling pathway; GO:0048246 macrophage chemotaxis; GO:0048247 lymphocyte chemotaxis; GO:0050806 positive regulation of synaptic transmission; GO:0051384 response to glucocorticoid stimulus; GO:0060137 maternal process involved in parturition; GO:0070098 chemokine-mediated signaling pathway
Regulatory: For research purposes only

Applications of MCP-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	0.5	2.0ug/ml
Functional Assays	4.0	6.0ug/ml
Immunohistology - Frozen
Western Blotting	0.1	0.2ug/ml

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems with the appropriate negative/positive controls.

Secondary Antibodies Available

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Goat anti Rabbit IgG (Fc):Biotin	STAR121B	E WB	1 mg	Log in	Get Quote
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Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	WB*	0.5 ml	Log in		Get Quote

Product Specific References

References for MCP-1 antibody

Zhang, H. et al. (2016) Dorsal root ganglion infiltration by macrophages contributes to paclitaxel chemotherapy induced peripheral neuropathy.
J Pain. Mar 12. pii: S1526-5900(16)00562-9. [Epub ahead of print]
Aharon-Hananel, G. et al. (2015) Antidiabetic Effect of Interleukin-1β Antibody Therapy Through β-Cell Protection in the Cohen Diabetes-Sensitive Rat.
Diabetes. 64 (5): 1780-5.
Schreiber, R.C. et al. (2001) Monocyte chemoattractant protein (MCP)-1 is rapidly expressed by sympathetic ganglion neurons following axonal injury.
Neuroreport. 12 (3): 601-6.
Tomas-sanchez, C. et al. (2018) Prophylactic Zinc and Therapeutic Selenium Administration Increases the Antioxidant Enzyme Activity in the Rat Temporoparietal Cortex and Improves Memory after a Transient Hypoxia-Ischemia.
Oxid Med Cell Longev. 2018: 9416432.
Rong, Y. et al. (2021) Small extracellular vesicles encapsulating CCL2 from activated astrocytes induce microglial activation and neuronal apoptosis after traumatic spinal cord injury.
J Neuroinflammation. 18 (1): 196.

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