MCP-1 antibody

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Rabbit anti Rat MCP-1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAR31 C E FN WB 0.1 mg
Rabbit anti Rat MCP-1 antibodyrecognizes rat monocyte chemo attractant protein-1 (MCP-1), also known as CCL-2.

Rat MCP-1 is a chemokine of approximately 12 kDa, which plays an important role in the inflammatory response of blood monocytes and tissue macrophages.

Product Details

Target Species
Rat
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to rat MCP-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant rat MCP-1.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution.

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P14844 Related reagents
Entrez Gene
Ccl2 Related reagents
GO Terms
GO:0001666 response to hypoxia
GO:0001938 positive regulation of endothelial cell proliferation
GO:0005615 extracellular space
GO:0005737 cytoplasm
GO:0006874 cellular calcium ion homeostasis
GO:0009612 response to mechanical stimulus
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007179 transforming growth factor beta receptor signaling pathway
GO:0007568 aging
GO:0008009 chemokine activity
GO:0008201 heparin binding
GO:0042493 response to drug
GO:0009408 response to heat
GO:0010332 response to gamma radiation
GO:0014823 response to activity
GO:0046677 response to antibiotic
GO:0030593 neutrophil chemotaxis
GO:0031100 organ regeneration
GO:0031727 CCR2 chemokine receptor binding
GO:0032496 response to lipopolysaccharide
GO:0032570 response to progesterone stimulus
GO:0033552 response to vitamin B3
GO:0042466 chemokinesis
GO:0043025 neuronal cell body
GO:0043200 response to amino acid stimulus
GO:0045471 response to ethanol
GO:0048010 vascular endothelial growth factor receptor signaling pathway
GO:0048246 macrophage chemotaxis
GO:0048247 lymphocyte chemotaxis
GO:0050806 positive regulation of synaptic transmission
GO:0051384 response to glucocorticoid stimulus
GO:0060137 maternal process involved in parturition
GO:0070098 chemokine-mediated signaling pathway
Regulatory
For research purposes only

Applications of MCP-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Functional Assays 4.0 6.0ug/ml
Immunohistology - Frozen
Western Blotting 0.1 0.2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems with the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for MCP-1 antibody

  1. Zhang, H. et al. (2016) Dorsal root ganglion infiltration by macrophages contributes to paclitaxel chemotherapy induced peripheral neuropathy.
    J Pain. Mar 12. pii: S1526-5900(16)00562-9. [Epub ahead of print]
  2. Aharon-Hananel, G. et al. (2015) Antidiabetic Effect of Interleukin-1β Antibody Therapy Through β-Cell Protection in the Cohen Diabetes-Sensitive Rat.
    Diabetes. 64 (5): 1780-5.
  3. Schreiber, R.C. et al. (2001) Monocyte chemoattractant protein (MCP)-1 is rapidly expressed by sympathetic ganglion neurons following axonal injury.
    Neuroreport. 12 (3): 601-6.