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MCP-1 antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E FN WB 0.1 mg loader
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Rabbit anti Rat MCP-1 antibodyrecognizes rat monocyte chemo attractant protein-1 (MCP-1), also known as CCL-2.

Rat MCP-1 is a chemokine of approximately 12 kDa, which plays an important role in the inflammatory response of blood monocytes and tissue macrophages.

Target Species
Product Form
Purified IgG - lyophilized
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to rat MCP-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Recombinant rat MCP-1.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution.
For research purposes only
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Functional Assays 4.0 6.0ug/ml
Immunohistology - Frozen
Western Blotting 0.1 0.2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems with the appropriate negative/positive controls.

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References for MCP-1 antibody

  1. Schreiber, R.C. et al. (2001) Monocyte chemoattractant protein (MCP)-1 is rapidly expressed by sympathetic ganglion neurons following axonal injury.
    Neuroreport. 12 (3): 601-6.
  2. Aharon-Hananel, G. et al. (2015) Antidiabetic Effect of Interleukin-1β Antibody Therapy Through β-Cell Protection in the Cohen Diabetes-Sensitive Rat.
    Diabetes. 64 (5): 1780-5.
  3. Zhang, H. et al. (2016) Dorsal Root Ganglion Infiltration by Macrophages Contributes to Paclitaxel Chemotherapy-Induced Peripheral Neuropathy.
    J Pain. 17 (7): 775-86.
  4. Tomas-Sanchez, C. et al. (2018) Prophylactic Zinc and Therapeutic Selenium Administration Increases the Antioxidant Enzyme Activity in the Rat Temporoparietal Cortex and Improves Memory after a Transient Hypoxia-Ischemia.
    Oxid Med Cell Longev. 2018: 9416432.
  5. Rong, Y. et al. (2021) Small extracellular vesicles encapsulating CCL2 from activated astrocytes induce microglial activation and neuronal apoptosis after traumatic spinal cord injury.
    J Neuroinflammation. 18 (1): 196.

Entrez Gene
GO Terms
GO:0001666 response to hypoxia
GO:0001938 positive regulation of endothelial cell proliferation
GO:0005615 extracellular space
GO:0005737 cytoplasm
GO:0006874 cellular calcium ion homeostasis
GO:0009612 response to mechanical stimulus
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007179 transforming growth factor beta receptor signaling pathway
GO:0007568 aging
GO:0008009 chemokine activity
GO:0008201 heparin binding
GO:0042493 response to drug
GO:0009408 response to heat
GO:0010332 response to gamma radiation
GO:0014823 response to activity
GO:0046677 response to antibiotic
GO:0030593 neutrophil chemotaxis
GO:0031100 organ regeneration
GO:0031727 CCR2 chemokine receptor binding
GO:0032496 response to lipopolysaccharide
GO:0032570 response to progesterone stimulus
GO:0033552 response to vitamin B3
GO:0042466 chemokinesis
GO:0043025 neuronal cell body
GO:0043200 response to amino acid stimulus
GO:0045471 response to ethanol
GO:0048010 vascular endothelial growth factor receptor signaling pathway
GO:0048246 macrophage chemotaxis
GO:0048247 lymphocyte chemotaxis
GO:0050806 positive regulation of synaptic transmission
GO:0051384 response to glucocorticoid stimulus
GO:0060137 maternal process involved in parturition
GO:0070098 chemokine-mediated signaling pathway


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