MCP-1 antibody
Rabbit anti Rat MCP-1 antibody (AAR31) used for the detection of MCP1 in rat temporoparietal cortex homogenates by ELISA.
Image caption:
The administration of the combined treatment with zinc and selenium modifies the protein levels of CCL2 in the rat hypoxia-ischemia model.CCAO: common carotid artery occlusion for 10 min; Zn + CCAO: chronic zinc administration before CCAO. Zn + CCAO + Se: chronic zinc administration before CCAO followed by selenium administration. The values were normalized against the control group. nv: normalized values. Each value represents of 5 independent experiments made in triplicate. , one-way ANOVA with post hoc Dunnett s test when compared with the untreated control group; , Student's -test when compared with the respective CCAO group.
From: Tomas-Sanchez C, et al.
Prophylactic Zinc and Therapeutic Selenium Administration Increases the Antioxidant Enzyme Activity in the Rat Temporoparietal Cortex and Improves Memory after a Transient Hypoxia-Ischemia.
Oxid Med Cell Longev. 2018:9416432.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rabbit anti Rat MCP-1 antibodyrecognizes rat monocyte chemo attractant protein-1 (MCP-1), also known as CCL-2.
Rat MCP-1 is a chemokine of approximately 12 kDa, which plays an important role in the inflammatory response of blood monocytes and tissue macrophages.
Rat MCP-1 is a chemokine of approximately 12 kDa, which plays an important role in the inflammatory response of blood monocytes and tissue macrophages.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide - Antiserum Preparation
- Antisera to rat MCP-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant rat MCP-1.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution.
Storage Information
- Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P14844
- Entrez Gene
- Ccl2
- GO Terms
- GO:0001666 response to hypoxia
- GO:0001938 positive regulation of endothelial cell proliferation
- GO:0005615 extracellular space
- GO:0005737 cytoplasm
- GO:0006874 cellular calcium ion homeostasis
- GO:0009612 response to mechanical stimulus
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0007179 transforming growth factor beta receptor signaling pathway
- GO:0007568 aging
- GO:0008009 chemokine activity
- GO:0008201 heparin binding
- GO:0042493 response to drug
- GO:0009408 response to heat
- GO:0010332 response to gamma radiation
- GO:0014823 response to activity
- GO:0046677 response to antibiotic
- GO:0030593 neutrophil chemotaxis
- GO:0031100 organ regeneration
- GO:0031727 CCR2 chemokine receptor binding
- GO:0032496 response to lipopolysaccharide
- GO:0032570 response to progesterone stimulus
- GO:0033552 response to vitamin B3
- GO:0042466 chemokinesis
- GO:0043025 neuronal cell body
- GO:0043200 response to amino acid stimulus
- GO:0045471 response to ethanol
- GO:0048010 vascular endothelial growth factor receptor signaling pathway
- GO:0048246 macrophage chemotaxis
- GO:0048247 lymphocyte chemotaxis
- GO:0050806 positive regulation of synaptic transmission
- GO:0051384 response to glucocorticoid stimulus
- GO:0060137 maternal process involved in parturition
- GO:0070098 chemokine-mediated signaling pathway
- Regulatory
- For research purposes only
Applications of MCP-1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.5 | 2.0ug/ml | |
| Functional Assays | 4.0 | 6.0ug/ml | |
| Immunohistology - Frozen | |||
| Western Blotting | 0.1 | 0.2ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems with the appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for MCP-1 antibody
-
Zhang, H. et al. (2016) Dorsal root ganglion infiltration by macrophages contributes to paclitaxel chemotherapy induced peripheral neuropathy.
J Pain. Mar 12. pii: S1526-5900(16)00562-9. [Epub ahead of print] -
Aharon-Hananel, G. et al. (2015) Antidiabetic Effect of Interleukin-1β Antibody Therapy Through β-Cell Protection in the Cohen Diabetes-Sensitive Rat.
Diabetes. 64 (5): 1780-5. -
Schreiber, R.C. et al. (2001) Monocyte chemoattractant protein (MCP)-1 is rapidly expressed by sympathetic ganglion neurons following axonal injury.
Neuroreport. 12 (3): 601-6. -
Tomas-sanchez, C. et al. (2018) Prophylactic Zinc and Therapeutic Selenium Administration Increases the Antioxidant Enzyme Activity in the Rat Temporoparietal Cortex and Improves Memory after a Transient Hypoxia-Ischemia.
Oxid Med Cell Longev. 2018: 9416432.
