IgM antibody
Horseradish peroxidase conjugated Goat anti Rat IgM antibody (STAR116P) used to evaluate post challenge antibody response in rats by ELISA.
Image caption:
Post-challenge serum antibody isotype responses of vaccinated and control rats to recombinant cysteine proteinase.
(A) IgG1. (B) IgM. (C) IgA. (D) IgE. Group G–Lyophilised lettuce expressing the mature CPFhW enzyme flanked by Gly-rich linkers; Group U–Lyophilised lettuce expressing the mature CPFhW protein fused with ubiquitin; Group C–Lyophilised control lettuce; Group N–None. At each timepoint, four rats from each group were euthanised and dissected. *Indicates significantly increased numbers of cells (p<0.05). Error bars indicate standard deviation.
From: Citation: Kesik-Brodacka M, Lipiec A, Kozak Ljunggren M, Jedlina L, Miedzinska K, Mikolajczak M, et al. (2017)
Immune response of rats vaccinated orally with various plant-expressed recombinant cysteine proteinase constructs when challenged with Fasciola hepatica metacercariae.
PLoS Negl Trop Dis 11(3): e0005451.
Goat anti Rat IgM antibody recognizes rat IgMas assessed by immunoelectrophoresis and ELISA.
Goat anti Rat IgM antibody may cross react with IgM from other species.
Goat anti Rat IgM antibody may cross react with IgM from other species.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to rat IgM were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.05% Proclin™ 300
0.2% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
- Guarantee
- 12 months from date of despatch.
More Information
- Acknowledgements
- Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
- Regulatory
- For research purposes only
Applications of IgM antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/10,000 | 1/100,000 | |
| Western Blotting | 1/1000 | 1/10,000 | |
| ELISA | 1ug/ml | 10ug/ml | |
| Flow Cytometry | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to stain 1 x 106 cells in 100ul.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for IgM antibody
-
Terrell, S.P. et al. (2012) Glomerulonephropathy in aged captive Key Largo woodrats (Neotoma floridana smalli).
Vet Pathol. 49 (4): 710-6. -
Kesik-brodacka, M. et al. (2017) Immune response of rats vaccinated orally with various plant-expressed recombinant cysteine proteinase constructs when challenged with Fasciola hepatica metacercariae.
PLoS Negl Trop Dis. 11 (3): e0005451. -
Bouma, H.R. et al. (2013) Hibernation is associated with depression of T-cell independent humoral immune responses in the 13-lined ground squirrel.
Dev Comp Immunol. 39 (3): 154-60.
