IgM antibody
Horseradish peroxidase conjugated Goat anti Rat IgM antibody (STAR116P) used to evaluate post challenge antibody response in rats by ELISA.
Image caption:
Post-challenge serum antibody isotype responses of vaccinated and control rats to recombinant cysteine proteinase.
(A) IgG1. (B) IgM. (C) IgA. (D) IgE. Group G–Lyophilised lettuce expressing the mature CPFhW enzyme flanked by Gly-rich linkers; Group U–Lyophilised lettuce expressing the mature CPFhW protein fused with ubiquitin; Group C–Lyophilised control lettuce; Group N–None. At each timepoint, four rats from each group were euthanised and dissected. *Indicates significantly increased numbers of cells (p<0.05). Error bars indicate standard deviation.
From: Citation: Kesik-Brodacka M, Lipiec A, Kozak Ljunggren M, Jedlina L, Miedzinska K, Mikolajczak M, et al. (2017)
Immune response of rats vaccinated orally with various plant-expressed recombinant cysteine proteinase constructs when challenged with Fasciola hepatica metacercariae.
PLoS Negl Trop Dis 11(3): e0005451.
Goat anti Rat IgM
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgM
Antibody Quality and Performance Guarantee
| Goat anti Rat IgM antibody recognizes rat IgMas assessed by immunoelectrophoresis and ELISA. Goat anti Rat IgM antibody may cross react with IgM from other species. |
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to rat IgM were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1ug/ml | 10ug/ml |
| Flow Cytometry | |
||
| Western Blotting | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to stain 1 x 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
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| Description | Rabbit anti Goat IgG (Fc):FITC | ||||||
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Rabbit anti Goat IgG (Fc):HRP | ||||||
References for IgM antibody
-
Terrell, S.P. et al. (2012) Glomerulonephropathy in aged captive Key Largo woodrats (Neotoma floridana smalli).
Vet Pathol. 49 (4): 710-6. -
Bouma, H.R. et al. (2013) Hibernation is associated with depression of T-cell independent humoral immune responses in the 13-lined ground squirrel.
Dev Comp Immunol. 39 (3): 154-60. -
Kesik-brodacka, M. et al. (2017) Immune response of rats vaccinated orally with various plant-expressed recombinant cysteine proteinase constructs when challenged with Fasciola hepatica metacercariae.
PLoS Negl Trop Dis. 11 (3): e0005451.
- RRID
- AB_567061
STAR116
158098
180703
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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