IgG2a antibody
No associated image currently available
Goat anti Rat IgG2a:Alk. Phos.
Goat anti Rat IgG2a:FITC
Goat anti Rat IgG2a:HRP
Goat anti Rat IgG2a
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| STAR113AB | E WB | 0.5 mg | ||||
| STAR113F | F | 0.5 mg | ||||
| STAR113P | E WB | 0.5 mg | ||||
| STAR113 | E F WB | 0.5 mg |
Goat anti Rat IgG2a antibody recognizes rat IgG2a as assessed by immunoelectrophoresis and ELISA.
Goat anti Rat IgG2a antibody may cross react with IgG2a from other species.
Goat anti Rat IgG2a antibody may cross react with IgG2a from other species.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Alkaline Phosphatase - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to rat IgG2a were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- 50mM HEPES, 0.1M NaCl, 1mM MgCl2, 0.1mM ZnCl2
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 0.2% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 0.2% Bovine Serum Albumin - Preservative Stabilisers
- 0.05% Proclin™ 300
0.2% Bovine Serum Albumin - Preservative Stabilisers
0.1% Sodium Azide - Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC.
DO NOT FREEZE
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P20760 Related reagents
- Entrez Gene
- LOC679045 Related reagents
- GO Terms
- GO:0003823 antigen binding
- Acknowledgements
- Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
- Regulatory
- For research purposes only
Applications of IgG2a antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/1000 | 1/10000 | |
| Western Blotting | 1/1000 | 1/10000 | |
| Flow Cytometry | 1/500 | ||
| ELISA | 1/10,000 | 1/100,000 | |
| Western Blotting | 1/2,000 | 1/20,000 | |
| ELISA | 1ug/ml | 10ug/ml | |
| Flow Cytometry | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 50ul of the suggested working dilution to label 1 x 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to stain 1 x 106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | 1 mg | F | ||
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | 1 mg | C E WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | 100 ml | C E P WB | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | 500 ml | C E P WB | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | 1000 ml | C E P WB | ||
| TMB Signal+ | BUF054A | 100 ml | E | ||
| TMB Core | BUF056A | 100 ml | E | ||
| TMB Core+ | BUF062A | 100 ml | E |
Product Specific References
References for IgG2a antibody
-
Platt, R. et al. (2011) Comparison of humoral and cellular immune responses to inactivated swine influenza virus vaccine in weaned pigs.
Vet Immunol Immunopathol. 142: 252-7.
