Glutamate Decarboxylase 1/2 antibody
Rabbit anti Glutamate Decarboxylase 1/2 antibody recognizes both molecular forms of glutamate decarboxylase, GAD1 (GAD67) and GAD2 (GAD65), the enzyme which converts glutamic acid to gamma -aminobutyric acid (GABA), the major inhibitory transmitter in the higher brain region.
Product Details
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Cat Human Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to glutamic acid decarboxylase was raised by repeated immunisation of with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on protein A.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide sequence D-F-L-I-E-E-I-E-R-L-G-Q-D-L from the C terminus of rat GAD2
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- Q05683 Related reagents
- Entrez Gene
- Gad2 Related reagents
- GO Terms
- GO:0000139 Golgi membrane
- GO:0042136 neurotransmitter biosynthetic process
- GO:0004351 glutamate decarboxylase activity
- GO:0005829 cytosol
- GO:0006540 glutamate decarboxylation to succinate
- GO:0042493 response to drug
- GO:0031225 anchored to membrane
- GO:0030054 cell junction
- GO:0030170 pyridoxal phosphate binding
- GO:0030672 synaptic vesicle membrane
- GO:0042734 presynaptic membrane
- GO:0048471 perinuclear region of cytoplasm
- Regulatory
- For research purposes only
Applications of Glutamate Decarboxylase 1/2 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
Immunofluorescence | |||
Immunohistology - Frozen | 1/200 | 1/5000 | |
Immunohistology - Paraffin | |||
Western Blotting | 1/1000 | 1/10000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Brain
- Western Blotting
- A doublet at approximately 65/67 kDa can be expected
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Description | Product Code | Pack Size | Applications | List Price | Quantity |
---|---|---|---|---|---|
Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
Description | Product Code | Pack Size | Applications | List Price | Quantity |
---|---|---|---|---|---|
Antigen Retrieval Buffer, pH8.0 | BUF025A | 500 ml | P | ||
Antigen Retrieval Buffer, pH8.0 | BUF025C | 50 ml | P | ||
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* |
Product Specific References
References for Glutamate Decarboxylase 1/2 antibody
-
Hernández-montiel, H.L. et al. (2003) Diffusible signals and fasciculated growth in reticulospinal axon pathfinding in the hindbrain.
Dev Biol. 255 (1): 99-112. -
Baizer, J.S. et al. (2011) Neurochemical and structural organization of the principal nucleus of the inferior olive in the human.
Anat Rec (Hoboken). 294: 1198-216. -
Klusa, V. et al. (2013) Mildronate enhances learning/memory and changes hippocampal protein expression in trained rats.
Pharmacol Biochem Behav. pii: S0091-3057(13)00078-6. -
Wang, X. et al. (2008) A novel expression platform for the production of diabetes-associated autoantigen human glutamic acid decarboxylase (hGAD65).
BMC Biotechnol. 8:87 -
Baizer, J.S. (2014) Unique features of the human brainstem and cerebellum.
Front Hum Neurosci. 8: 202.