Glutamate Decarboxylase 1/2 antibody
Rabbit anti glutamate decarboxylase 1/2 antibody (AHP360) used for the evaluation of hGAD65 expression levels in Gad65 expressing algal transformants by sandwich ELISA.
Image caption:
Expression levels as percentages of hGAD65 in total soluble protein (TSP) of hGAD65 expressing C. reinhardtii transformants. The expression level was determined by hGAD65 specific ELISA as described in detail in methods. hGAD-1 and hGAD-2, two independent algal transformants; WT, untransformed wild type C. reinhardtii 137c strain.
From:Wang X, Brandsma M, Tremblay R, Maxwell D, Jevnikar AM, Huner N, Ma S. A novel expression platform for the production of diabetes-associated autoantigen human glutamic acid decarboxylase (hGAD65).
BMC Biotechnol. 2008 Nov 17;8:87.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rabbit anti Glutamate Decarboxylase 1/2
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Glutamate Decarboxylase 1/2
- Region
- 65/67kDa
Antibody Quality and Performance Guarantee
| Rabbit anti Glutamate Decarboxylase 1/2 antibody recognizes both molecular forms of glutamate decarboxylase, GAD1 (GAD67) and GAD2 (GAD65), the enzyme which converts glutamic acid to gamma -aminobutyric acid (GABA), the major inhibitory transmitter in the higher brain region. |
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Cat Human Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to glutamic acid decarboxylase was raised by repeated immunisation of with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on protein A.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide sequence D-F-L-I-E-E-I-E-R-L-G-Q-D-L from the C terminus of rat GAD2
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
1/200 | 1/5000 |
| Immunohistology - Paraffin | |
||
| Western Blotting | |
1/1000 | 1/10000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Brain
- Western Blotting
- A doublet at approximately 65/67 kDa can be expected
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | Antigen Retrieval Buffer, pH8.0 | ||||||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
References for Glutamate Decarboxylase 1/2 antibody
-
Hernández-montiel, H.L. et al. (2003) Diffusible signals and fasciculated growth in reticulospinal axon pathfinding in the hindbrain.
Dev Biol. 255 (1): 99-112. -
Wang, X. et al. (2008) A novel expression platform for the production of diabetes-associated autoantigen human glutamic acid decarboxylase (hGAD65).
BMC Biotechnol. 8:87 -
Baizer, J.S. et al. (2011) Neurochemical and structural organization of the principal nucleus of the inferior olive in the human.
Anat Rec (Hoboken). 294: 1198-216. -
Klusa, V. et al. (2013) Mildronate enhances learning/memory and changes hippocampal protein expression in trained rats.
Pharmacol Biochem Behav. 106: 68-76. -
Baizer, J.S. (2014) Unique features of the human brainstem and cerebellum.
Front Hum Neurosci. 8: 202. -
Solís-S, J.C. et al. (2018) Signals from the caudal diencephalon are required for the projection of the Interstitial Nuclei of Cajal.
Int J Dev Neurosci. 69: 10-6.
- Synonyms
- GAD1
- GAD2
- RRID
- AB_322069
- UniProt
- Q05683
- Entrez Gene
- Gad2
- GO Terms
- GO:0000139 Golgi membrane
- GO:0042136 neurotransmitter biosynthetic process
- GO:0004351 glutamate decarboxylase activity
- GO:0005829 cytosol
- GO:0006540 glutamate decarboxylation to succinate
- GO:0042493 response to drug
- GO:0031225 anchored to membrane
- GO:0030054 cell junction
- GO:0030170 pyridoxal phosphate binding
- View More GO Terms
- GO:0030672 synaptic vesicle membrane
- GO:0042734 presynaptic membrane
- GO:0048471 perinuclear region of cytoplasm
AHP360
148407
150756
157118
168647
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