Glutamate Decarboxylase 1/2 antibody

Rabbit anti Glutamate Decarboxylase 1/2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Glutamate Decarboxylase 1/2
Region
65/67kDa

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AHP360
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C E IF P WB 0.1 ml loader
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Rabbit anti Glutamate Decarboxylase 1/2 antibody recognizes both molecular forms of glutamate decarboxylase, GAD1 (GAD67) and GAD2 (GAD65), the enzyme which converts glutamic acid to gamma -aminobutyric acid (GABA), the major inhibitory transmitter in the higher brain region.

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cat
Human
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to glutamic acid decarboxylase was raised by repeated immunisation of with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on protein A.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide sequence D-F-L-I-E-E-I-E-R-L-G-Q-D-L from the C terminus of rat GAD2
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunohistology - Frozen 1/200 1/5000
Immunohistology - Paraffin
Western Blotting 1/1000 1/10000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Brain
Western Blotting
A doublet at approximately 65/67 kDa can be expected

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References for Glutamate Decarboxylase 1/2 antibody

  1. Hernández-montiel, H.L. et al. (2003) Diffusible signals and fasciculated growth in reticulospinal axon pathfinding in the hindbrain.
    Dev Biol. 255 (1): 99-112.
  2. Baizer, J.S. et al. (2011) Neurochemical and structural organization of the principal nucleus of the inferior olive in the human.
    Anat Rec (Hoboken). 294: 1198-216.
  3. Klusa, V. et al. (2013) Mildronate enhances learning/memory and changes hippocampal protein expression in trained rats.
    Pharmacol Biochem Behav. pii: S0091-3057(13)00078-6.
  4. Wang, X. et al. (2008) A novel expression platform for the production of diabetes-associated autoantigen human glutamic acid decarboxylase (hGAD65).
    BMC Biotechnol. 8:87
  5. Baizer, J.S. (2014) Unique features of the human brainstem and cerebellum.
    Front Hum Neurosci. 8: 202.
  6. Solís-S, J.C. et al. (2018) Signals from the caudal diencephalon are required for the projection of the Interstitial Nuclei of Cajal.
    Int J Dev Neurosci. 69: 10-6.

Synonyms
GAD1
GAD2
RRID
AB_322069
UniProt
Q05683
Entrez Gene
Gad2
GO Terms
GO:0000139 Golgi membrane
GO:0042136 neurotransmitter biosynthetic process
GO:0004351 glutamate decarboxylase activity
GO:0005829 cytosol
GO:0006540 glutamate decarboxylation to succinate
GO:0042493 response to drug
GO:0031225 anchored to membrane
GO:0030054 cell junction
GO:0030170 pyridoxal phosphate binding
GO:0030672 synaptic vesicle membrane
GO:0042734 presynaptic membrane
GO:0048471 perinuclear region of cytoplasm