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Glutamate Decarboxylase 1/2 antibody

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Anti Glutamate Decarboxylase 1/2 Antibody gallery image 1
Published customer image:
Rabbit anti glutamate decarboxylase 1/2 antibody used for the evaluation of hGAD65 expression levels in Gad65 expressing algal transformants by sanwich ELISA.
Image caption:
Expression levels as percentages of hGAD65 in total soluble protein (TSP) of hGAD65 expressing C. reinhardtii transformants. The expression level was determined by hGAD65 specific ELISA as described in detail in methods. hGAD-1 and hGAD-2, two independent algal transformants; WT, untransformed wild type C. reinhardtii 137c strain.

From:Wang X, Brandsma M, Tremblay R, Maxwell D, Jevnikar AM, Huner N, Ma S. A novel expression platform for the production of diabetes-associated autoantigen human glutamic acid decarboxylase (hGAD65).
BMC Biotechnol. 2008 Nov 17;8:87.

Rabbit anti Glutamate Decarboxylase 1/2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP360 C E IF P WB 0.1 ml
Rabbit anti Glutamate Decarboxylase 1/2 antibody recognizes both molecular forms of glutamate decarboxylase, GAD1 (GAD67) and GAD2 (GAD65), the enzyme which converts glutamic acid to gamma -aminobutyric acid (GABA), the major inhibitory transmitter in the higher brain region.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cat
Human
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to glutamic acid decarboxylase was raised by repeated immunisation of with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on protein A.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide sequence D-F-L-I-E-E-I-E-R-L-G-Q-D-L from the C terminus of rat GAD2

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q05683 Related reagents
Entrez Gene
Gad2 Related reagents
GO Terms
GO:0000139 Golgi membrane
GO:0042136 neurotransmitter biosynthetic process
GO:0004351 glutamate decarboxylase activity
GO:0005829 cytosol
GO:0006540 glutamate decarboxylation to succinate
GO:0042493 response to drug
GO:0031225 anchored to membrane
GO:0030054 cell junction
GO:0030170 pyridoxal phosphate binding
GO:0030672 synaptic vesicle membrane
GO:0042734 presynaptic membrane
GO:0048471 perinuclear region of cytoplasm
Regulatory
For research purposes only

Applications of Glutamate Decarboxylase 1/2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunohistology - Frozen 1/200 1/5000
Immunohistology - Paraffin
Western Blotting 1/1000 1/10000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Brain
Western Blotting
A doublet at approximately 65/67 kDa can be expected
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Application Based External Images

ELISA

Product Specific References

References for Glutamate Decarboxylase 1/2 antibody

  1. Hernandez-Montiel, H.L. et al. (2003) Diffusible signals and fasciculated growth in reticulospinal axon pathfinding in the hindbrain.
    Dev. Biol. 255: 99-112.
  2. Baizer, J.S. et al. (2011) Neurochemical and structural organization of the principal nucleus of the inferior olive in the human.
    Anat Rec (Hoboken). 294: 1198-216.
  3. Klusa, V. et al. (2013) Mildronate enhances learning/memory and changes hippocampal protein expression in trained rats.
    Pharmacol Biochem Behav. pii: S0091-3057(13)00078-6.
  4. Wang, X. et al. (2008) A novel expression platform for the production of diabetes-associated autoantigen human glutamic acid decarboxylase (hGAD65).
    BMC Biotechnol. 8:87
  5. Baizer, J.S. (2014) Unique features of the human brainstem and cerebellum.
    Front Hum Neurosci. 8: 202.
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