G Protein Alpha 12 antibody

Rabbit anti G Protein Alpha 12

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
G Protein Alpha 12

Product Code Applications Pack Size List Price Your Price Qty
4737-2210
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 1 ml loader
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Rabbit anti G protein alpha 12 antibody recognizes G protein alpha 12, a guanine nucleotide binding protein that is involved in various transmembrane signaling systems. G proteins are composed of 3 units (alpha, beta and gamma); the alpha chain contains the guanine nucleotide binding site.

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human Expected from Sequence
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to the amino acids 370 - 379 (QENLKDIMLQ) of rat G protein alpha 12 conjugated to Keyhole Limpet Haemocyanin.
Approx. Protein Concentrations
IgG concentration 5.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/20 1/500
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Western Blotting
4737-2210 detects a band of approximately 43kDa in rat brain when used under reducing conditions at a dilution of 1/500

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RRID
AB_2232425
UniProt
Q63210
Entrez Gene
Gna12
GO Terms
GO:0005834 heterotrimeric G-protein complex
GO:0004871 signal transducer activity
GO:0005525 GTP binding
GO:0007212 dopamine receptor signaling pathway
GO:0042493 response to drug
GO:0031526 brush border membrane
GO:0031752 D5 dopamine receptor binding
Request a different product with this specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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