G Protein Alpha 12 antibody

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Rabbit anti G Protein Alpha 12

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
4737-2210 E WB 1 ml
Rabbit anti G protein alpha 12 antibody recognizes G protein alpha 12, a guanine nucleotide binding protein that is involved in various transmembrane signaling systems. G proteins are composed of 3 units (alpha, beta and gamma); the alpha chain contains the guanine nucleotide binding site.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human Expected from Sequence
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to the amino acids 370 - 379 (QENLKDIMLQ) of rat G protein alpha 12 conjugated to Keyhole Limpet Haemocyanin.
Approx. Protein Concentrations
IgG concentration 5.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from the date of despatch.

More Information

UniProt
Q63210 Related reagents
Entrez Gene
Gna12 Related reagents
GO Terms
GO:0005834 heterotrimeric G-protein complex
GO:0004871 signal transducer activity
GO:0005525 GTP binding
GO:0007212 dopamine receptor signaling pathway
GO:0042493 response to drug
GO:0031526 brush border membrane
GO:0031752 D5 dopamine receptor binding
Regulatory
For research purposes only

Applications of G Protein Alpha 12 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/20 1/500
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Western Blotting
4737-2210 detects a band of approximately 43kDa in rat brain when used under reducing conditions at a dilution of 1/500.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*