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Connexin 43 (pSer368) antibody

Rabbit anti Connexin 43 (pSer368)

Product Type
Polyclonal Antibody
Polyclonal IgG
Connexin 43 (pSer368)

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 ml loader
List Price Your Price

Rabbit anti Rat connexin 43 (pSer368) antibody recognizes connexin 43 (Cx43), also known as gap junction alpha-1 protein and gap junction 43 kDa heart protein, when phosphorylated at serine 368.

Connexin 43 allows for the exchange of solutions between neighbouring cells through the formation of gap-junctional channels and is expressed in the brain, kidneys and myocardium, as well as vascular endothelial cells. A decrease in the permeability of gap-junctional channels occurs due to a conformational change in the structure of connexin 43, and that this is mediated by the phosphorylation of connexin 43 at residue serine 368, by Protein Kinase C (Bao et al. 2004).

A possible link between connexin 43 and epilepsy has been investigated following the detection of high levels of connexin 43 in both low-grade tumours and peritumoural reactive astrocytes (Aronica et al. 2001).

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Zebrafish Expected from Sequence
Chicken Expected from Sequence
Guinea Pig Expected from Sequence
Mouse Expected from Sequence
Dog Expected from Sequence
Human Expected from Sequence
Bovine Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to rat connexin43 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
10mM HEPES pH7.5
Preservative Stabilisers
0.09%Sodium Azide
0.01%Bovine Serum Albumin
Synthetic phosphopeptide corresponding to an amino acid sequence within connexin43 which includes phosphorylated Ser368
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP977 detects a band of approximately 43kDa in rat hippocampus cell lysates.

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Further Reading

  1. Bao, X. et al. (2004) Mechanism of regulation of the gap junction protein connexin 43 by protein kinase C-mediated phosphorylation.
    Am J Physiol Cell Physiol. 286 (3): C647-54.
  2. Bao, X. et al. (2004) Regulation of purified and reconstituted connexin 43 hemichannels by protein kinase C-mediated phosphorylation of Serine 368.
    J Biol Chem. 279 (19): 20058-66.
  3. Aronica, E. et al. (2001) Expression of connexin 43 and connexin 32 gap-junction proteins in epilepsy-associated brain tumors and in the perilesional epileptic cortex.
    Acta Neuropathol. 101 (5): 449-59.

Gap Junction ALPHA-1 Protein
Entrez Gene
GO Terms
GO:0006915 apoptosis
GO:0005764 lysosome
GO:0005887 integral to plasma membrane
GO:0005243 gap junction channel activity
GO:0005741 mitochondrial outer membrane
GO:0005769 early endosome
GO:0005771 multivesicular body
GO:0005789 endoplasmic reticulum membrane
GO:0005916 fascia adherens
GO:0005922 connexon complex
GO:0007267 cell-cell signaling
GO:0008285 negative regulation of cell proliferation
GO:0009268 response to pH
GO:0010232 vascular transport
GO:0015867 ATP transport
GO:0016264 gap junction assembly
GO:0017124 SH3 domain binding
GO:0030165 PDZ domain binding
GO:0030660 Golgi-associated vesicle membrane
GO:0043434 response to peptide hormone stimulus
GO:0045121 membrane raft
GO:0045732 positive regulation of protein catabolic process
GO:0048812 neuron projection morphogenesis
GO:0051259 protein oligomerization
GO:0051924 regulation of calcium ion transport


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