Connexin 43 (pSer368) antibody

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Rabbit anti Connexin 43 (pSer368)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP977 WB* 0.1 ml
Rabbit anti Rat connexin 43 (pSer368) antibody recognizes connexin 43 (Cx43), also known as gap junction alpha-1 protein and gap junction 43 kDa heart protein, when phosphorylated at serine 368.

Connexin 43 allows for the exchange of solutions between neighbouring cells through the formation of gap-junctional channels and is expressed in the brain, kidneys and myocardium, as well as vascular endothelial cells. A decrease in the permeability of gap-junctional channels occurs due to a conformational change in the structure of connexin 43, and that this is mediated by the phosphorylation of connexin 43 at residue serine 368, by Protein Kinase C (Bao et al. 2004).

A possible link between connexin 43 and epilepsy has been investigated following the detection of high levels of connexin 43 in both low-grade tumours and peritumoural reactive astrocytes (Aronica et al. 2001).

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Zebrafish Expected from Sequence
Chicken Expected from Sequence
Guinea Pig Expected from Sequence
Mouse Expected from Sequence
Dog Expected from Sequence
Human Expected from Sequence
Bovine Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to rat connexin43 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
10mM HEPES pH7.5
Preservative Stabilisers
0.05%Sodium Azide
0.01%Bovine Serum Albumin
50%Glycerol
Immunogen
Synthetic phosphopeptide corresponding to an amino acid sequence within connexin43 which includes phosphorylated Ser368

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
P08050 Related reagents
Entrez Gene
Gja1 Related reagents
GO Terms
GO:0006915 apoptosis
GO:0005764 lysosome
GO:0005887 integral to plasma membrane
GO:0005243 gap junction channel activity
GO:0005741 mitochondrial outer membrane
GO:0005769 early endosome
GO:0005771 multivesicular body
GO:0005789 endoplasmic reticulum membrane
GO:0005916 fascia adherens
GO:0005922 connexon complex
GO:0007267 cell-cell signaling
GO:0008285 negative regulation of cell proliferation
GO:0009268 response to pH
GO:0010232 vascular transport
GO:0015867 ATP transport
GO:0016264 gap junction assembly
GO:0017124 SH3 domain binding
GO:0030165 PDZ domain binding
GO:0030660 Golgi-associated vesicle membrane
GO:0043434 response to peptide hormone stimulus
GO:0045121 membrane raft
GO:0045732 positive regulation of protein catabolic process
GO:0048812 neuron projection morphogenesis
GO:0051259 protein oligomerization
GO:0051924 regulation of calcium ion transport
Regulatory
For research purposes only

Applications of Connexin 43 (pSer368) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1 1/1000
  1. 1 For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP977 detects a band of approximately 43kDa in rat hippocampus cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Product Specific References

Further Reading

  1. Bao, X. et al. (2004) Mechanism of regulation of the gap junction protein connexin 43 by protein kinase C-mediated phosphorylation.
    Am J Physiol Cell Physiol. 286 (3): C647-54.
  2. Bao, X. et al. (2004) Regulation of purified and reconstituted connexin 43 hemichannels by protein kinase C-mediated phosphorylation of Serine 368.
    J Biol Chem. 279 (19): 20058-66.
  3. Aronica, E. et al. (2001) Expression of connexin 43 and connexin 32 gap-junction proteins in epilepsy-associated brain tumors and in the perilesional epileptic cortex.
    Acta Neuropathol. 101 (5): 449-59.