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Collagen III antibody

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Rabbit anti Rat Collagen III

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
2150-1950
Datasheet
C E IF P R
SDS
0.1 ml loader

Rabbit anti Rat Collagen III antibody recognises rat collagen type III, a fibrillar collagen found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen.

Rabbit anti Rat Collagen III antibody (2150-1950) shows less than 10% cross-reactivity with collagen type V and minimal reactivity with rat collagen type I and elastin.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Antibiotic antimycotic mixture 1%
Immunogen
Purified collagen III from rat skin.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P13941
Entrez Gene
Col3a1
GO Terms
GO:0001501 skeletal system development
GO:0005586 collagen type III
Regulatory
For research purposes only

Applications of Collagen III antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/400
Immunofluorescence 1/80
Immunohistology - Frozen 1/250 1/1000
Immunohistology - Paraffin 1/250 1/1000
Radioimmunoassays 1/25 1/100
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
100x Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml

Product Specific References

References for Collagen III antibody

  1. Vialle-presles, M.J. et al. (1989) Immunohistochemical study of the biological fate of a subcutaneous bovine collagen implant in rat.
    Histochemistry. 91 (3): 177-84.
  2. Dankers, P.Y.W. et al. (2015) Convenient formulation and application of a supramolecular ureido-pyrimidinone modified poly(ethylene glycol) carrier for intrarenal growth factor delivery
    European Polymer Journal. 72: 484-493.
  3. Oshita, T. et al. (2016) Adipose-Derived Stem Cells Improve Collagenase-Induced Tendinopathy in a Rat Model.
    Am J Sports Med. 44 (8): 1983-9.

Further Reading

  1. Riser, B.L. et al. (1992) Intraglomerular pressure and mesangial stretching stimulate extracellular matrix formation in the rat.
    J Clin Invest. 90 (5): 1932-43.

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