Collagen III antibody
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Rabbit anti Rat Collagen III antibody recognises rat collagen type III, a fibrillar collagen found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen.
Rabbit anti Rat Collagen III antibody (2150-1950) shows less than 10% cross-reactivity with collagen type V and minimal reactivity with rat collagen type I and elastin.
Rabbit anti Rat Collagen III antibody (2150-1950) shows less than 10% cross-reactivity with collagen type V and minimal reactivity with rat collagen type I and elastin.
Product Details
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
Antibiotic antimycotic mixture 1% - Immunogen
- Purified collagen III from rat skin.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P13941
- Entrez Gene
- Col3a1
- GO Terms
- GO:0001501 skeletal system development
- GO:0005586 collagen type III
- Regulatory
- For research purposes only
Applications of Collagen III antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/100 | 1/400 | |
| Immunofluorescence | 1/80 | ||
| Immunohistology - Frozen | 1/250 | 1/1000 | |
| Immunohistology - Paraffin | 1/250 | 1/1000 | |
| Radioimmunoassays | 1/25 | 1/100 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml |
Product Specific References
References for Collagen III antibody
-
Vialle-presles, M.J. et al. (1989) Immunohistochemical study of the biological fate of a subcutaneous bovine collagen implant in rat.
Histochemistry. 91 (3): 177-84. -
Dankers, P.Y.W. et al. (2015) Convenient formulation and application of a supramolecular ureido-pyrimidinone modified poly(ethylene glycol) carrier for intrarenal growth factor delivery
European Polymer Journal. 72: 484-493. -
Oshita, T. et al. (2016) Adipose-Derived Stem Cells Improve Collagenase-Induced Tendinopathy in a Rat Model.
Am J Sports Med. 44 (8): 1983-9.
Further Reading
-
Riser, B.L. et al. (1992) Intraglomerular pressure and mesangial stretching stimulate extracellular matrix formation in the rat.
J Clin Invest. 90 (5): 1932-43.
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