Alpha Synuclein (pSer129) antibody

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Rabbit anti Alpha Synuclein (pSer129)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1537
Datasheet
WB
MSDS
0.1 ml loader

Rabbit anti Rat alpha synuclein (pSer129) antibody recognizes alpha synuclein when phosphorylated at serine 129. Alpha synuclein is a 140 amino acid, ~15 kDa protein seen predominantly in the neocortex, hippocampus, substantia nigra, thalamus and cerebellum. Its normal cellular function has not been definitively determined, but it is thought to be involved in the regulation of neuronal plasticity, dopamine neurotransmission and other membrane-associated processes at the presynaptic terminal.

Alpha synuclein is normally a soluble, unfolded protein, but it can aggregate at high concentrations to form insoluble fibrils which, in association with other proteins such as ubiquitin, neurofilament protein and alpha B crystalline, form Lewy bodies. Lewy bodies are a hallmark pathological feature of Parkinson's disease and other neurodegenerative diseases. Alpha synuclein has several possible phosphorylation sites, serine 129 in particular can be phosphorylated by G-protein-coupled receptor kinases and this phosphorylation promotes formation of alpha synuclein filaments, with hyperphosphorylation of serine129 seen in Lewy bodies.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Dog Expected from Sequence
Primate Expected from Sequence
Human Expected from Sequence
Bovine Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Alpha Synulein (pSer129) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
10mM Hepes pH7.5, 150mM NaCl
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
50% Glycerol
0.01%Bovine Serum Albumin
Immunogen
Synthetic phosphopeptide corresponding to amino acid sequence surrounding phosphorylated serine 129 of Alpha Synuclein.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P37377 Related reagents
Entrez Gene
Snca Related reagents
GO Terms
GO:0001933 negative regulation of protein phosphorylation
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0005543 phospholipid binding
GO:0005625 soluble fraction
GO:0005792 microsome
GO:0008017 microtubule binding
GO:0008021 synaptic vesicle
GO:0048148 behavioral response to cocaine
GO:0014059 regulation of dopamine secretion
GO:0019904 protein domain specific binding
GO:0030659 cytoplasmic vesicle membrane
GO:0043195 terminal button
GO:0043274 phospholipase binding
GO:0043524 negative regulation of neuron apoptosis
GO:0045963 negative regulation of dopamine metabolic process
GO:0047485 protein N-terminus binding
Regulatory
For research purposes only

Applications of Alpha Synuclein (pSer129) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1537 detects a band of approximately 15kDa in rat cortex lysate.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B C E P WB 1 ml loader
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg loader
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg loader
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg loader

Product Specific References

References for Alpha Synuclein (pSer129) antibody

  1. Smith, W. W.et al (2005) Alpha-Synuclein Phosphorylation Enhances Eosinophilic Cytoplasmic Inclusion Formation in SH-SY5Y Cells.
    J. Neurosci. 25: 5544-52.