Alpha Synuclein (pSer129) antibody
Rabbit anti Alpha Synuclein (pSer129)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Alpha Synuclein (pSer129)
Rabbit anti Rat alpha synuclein (pSer129) antibody recognizes alpha synuclein when phosphorylated at serine 129. Alpha synuclein is a 140 amino acid, ~15 kDa protein seen predominantly in the neocortex, hippocampus, substantia nigra, thalamus and cerebellum. Its normal cellular function has not been definitively determined, but it is thought to be involved in the regulation of neuronal plasticity, dopamine neurotransmission and other membrane-associated processes at the presynaptic terminal. Alpha synuclein is normally a soluble, unfolded protein, but it can aggregate at high concentrations to form insoluble fibrils which, in association with other proteins such as ubiquitin, neurofilament protein and alpha B crystalline, form Lewy bodies. Lewy bodies are a hallmark pathological feature of Parkinson's disease and other neurodegenerative diseases. Alpha synuclein has several possible phosphorylation sites, serine 129 in particular can be phosphorylated by G-protein-coupled receptor kinases and this phosphorylation promotes formation of alpha synuclein filaments, with hyperphosphorylation of serine129 seen in Lewy bodies. |
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Dog Expected from Sequence Primate Expected from Sequence Human Expected from Sequence Bovine Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to Alpha Synulein (pSer129) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- 10mM Hepes pH7.5, 150mM NaCl
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) 50% Glycerol 0.01% Bovine Serum Albumin - Immunogen
- Synthetic phosphopeptide corresponding to amino acid sequence surrounding phosphorylated serine 129 of Alpha Synuclein.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Western Blotting | 1/1000 |
- Western Blotting
- AHP1537 detects a band of approximately 15kDa in rat cortex lysate.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg | Log in | |||
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Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg | Log in | |||
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Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg | Log in | |||
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Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg | Log in | |||
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Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml | Log in | |||
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Description | Sheep anti Rabbit IgG:RPE |
References for Alpha Synuclein (pSer129) antibody
-
Smith, W. W.et al (2005) Alpha-Synuclein Phosphorylation Enhances Eosinophilic Cytoplasmic Inclusion Formation in SH-SY5Y Cells.
J. Neurosci. 25: 5544-52.
- RRID
- AB_2192961
- UniProt
- P37377
- Entrez Gene
- Snca
- GO Terms
- GO:0001933 negative regulation of protein phosphorylation
- GO:0005739 mitochondrion
- GO:0005829 cytosol
- GO:0005543 phospholipid binding
- GO:0005625 soluble fraction
- GO:0005792 microsome
- GO:0008017 microtubule binding
- GO:0008021 synaptic vesicle
- GO:0048148 behavioral response to cocaine
- View More GO Terms
- GO:0014059 regulation of dopamine secretion
- GO:0019904 protein domain specific binding
- GO:0030659 cytoplasmic vesicle membrane
- GO:0043195 terminal button
- GO:0043274 phospholipase binding
- GO:0043524 negative regulation of neuron apoptosis
- GO:0045963 negative regulation of dopamine metabolic process
- GO:0047485 protein N-terminus binding
AHP1537
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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