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IgG antibody

Goat anti Rabbit IgG (Fc)

Product Type
Polyclonal Antibody
Polyclonal IgG

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 1 mg loader
List Price Your Price

Goat anti rabbit IgG antibody recognizes the Fc region of rabbit immunoglobulin G, confirmed by ELISA and immunoelectrophoresis. The antibody has not been species cross adsorbed and may cross react with IgG from other species. It shows weak recognition of rabbit IgM in ELISA.

Goat anti rabbit IgG antibody has been used successfully as a secondary detection reagent for rabbit primary antibodies using immunohistochemical techniques (Redondo et al. 2013).

Target Species
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to rabbit IgG were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
For research purposes only
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/ 100 1/ 10,000
Western Blotting 1/ 1000 1/ 10,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for IgG antibody

  1. Kalluri, S.R. et al. (2010) Quantification and functional characterization of antibodies to native aquaporin 4 in neuromyelitis optica.
    Arch Neurol. 67:1201-8.
  2. Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.
    J Comp Pathol. 150 (4): 434-48.
  3. Tyrsina, E. et al. (2019) Detection and quantification of VEGFR-1 in the nuclei of tumor cells by a new flow cytometry-based method.
    J Immunotoxicol. 16 (1): 74-81.
  4. Lin, W. et al. (2020) Rapid identification of anti-idiotypic mAbs with high affinity and diverse epitopes by rabbit single B-cell sorting-culture and cloning technology.
    PLoS One. 15 (12): e0244158.
  5. Li, Y. et al. (2022) Low-Temperature Plasma-Activated Medium Inhibited Proliferation and Progression of Lung Cancer by Targeting the PI3K/Akt and MAPK Pathways.
    Oxid Med Cell Longev. 2022: 9014501.

Entrez Gene
GO Terms
GO:0003823 antigen binding


150179 156041 158977 161658 167252 167283

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