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IgM antibody

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AAI48
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SDS Safety Datasheet SDS
C E * WB 1 mg loader
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loader

Goat anti Pig IgM antibody recognizes porcine IgM and shows no cross-reactivity with other porcine immunoglobulin classes in immunoelectrophoresis. This antibody may cross-react with IgM from other species.

Target Species
Pig
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to porcine IgM were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography using antigen coupled to agarose beads.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified Porcine IgM
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1 1/1000 1/30,000
Immunohistology - Frozen 1/200 1/2000
Western Blotting 1/1000 1/30,000
  1. 1For coating plates a 1/100 - 1/500 dilution is recommended.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for IgM antibody

  1. Williams, A.R. et al. (2017) Dietary cinnamaldehyde enhances acquisition of specific antibodies following helminth infection in pigs.
    Vet Immunol Immunopathol. 189: 43-52.
  2. Corsaut, L. et al. (2020) Field Study on the Immunological Response and Protective Effect of a Licensed Autogenous Vaccine to Control Streptococcus suis Infections in Post-Weaned Piglets.
    Vaccines (Basel). 8 (3)Jul 14 [Epub ahead of print].
  3. Corsaut, L. et al. (2021) Immunogenicity study of a Streptococcus suis. autogenous vaccine in preparturient sows and evaluation of passive maternal immunity in piglets.
    BMC Vet Res. 17 (1): 72.
  4. Forner, R. et al. (2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows.
    PLoS One. 16 (5): e0249366.
  5. López-Serrano, S. et al. (2023) Immune responses following neonatal vaccination with conserved F4 fragment of VtaA proteins from virulent Glaesserella parasuis adjuvanted with CAF®01 or CDA.
    Vaccine X. 14: 100330.
  6. Fabà, L. et al. (2023) Metabolic insights and background from naturally affected pigs during Streptococcus suis outbreaks
    Translational Animal Science. txad126.
  7. Urbano, A.C. et al. (2023) Targeted mutagenesis of the β-strand DNA binding region of African swine fever virus histone-like protein (pA104R) impairs DNA-binding activity and antibody recognition.
    Antiviral Res. : 105784.

AAI48

149925 156668 158090 159285 165004

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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