IgM antibody
HRP conjugated Goat anti Pig IgM antibody (AAI48P) used to evaluate IgM levels in porcine serum samples by ELISA.
Image caption:
Experiment 1: Isotype profile of antibodies against S. suis serotype 7 in vaccinated sows and their piglets. Blood samples were collected 7 weeks and 1 week before farrowing from 25 vaccinated sows to analyze the immune response. The vaccination protocol is shown in Fig. Fig.1.1. IgM (a) titers were determined by ELISA. Randomly selected piglets (n = 125; 5 piglets/sow) from vaccinated sows were sampled at 7 days of age to evaluate titers of IgM (d) against S. suis serotype 7. Individual antibody titers are shown with horizontal bars representing mean ± SEM. Values significantly different are shown in the graphs with corresponding P value.
From: Corsaut L, Martelet L, Goyette-Desjardins G, Beauchamp G, Denicourt M, Gottschalk M, Segura M.
Immunogenicity study of a Streptococcus suis autogenous vaccine in preparturient sows and evaluation of passive maternal immunity in piglets.
BMC Vet Res. 2021 Feb 5;17(1):72.
doi: 10.1186/s12917-021-02774-4..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
E ResetGoat anti Pig IgM
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgM
Antibody Quality and Performance Guarantee
| Goat anti Pig IgM antibody recognizes porcine IgM and shows no cross-reactivity with other porcine immunoglobulin classes in immunoelectrophoresis. This antibody may cross-react with IgM from other species. |
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to porcine IgM were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography using antigen coupled to agarose beads.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Purified Porcine IgM
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted.
This product should be stored undiluted.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA 1 |  |
1/1000 | 1/30,000 |
| Immunohistology - Frozen | |
1/200 | 1/2000 |
| Western Blotting | |
1/1000 | 1/30,000 |
- 1For coating plates a 1/100 - 1/500 dilution is recommended.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
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| Description | Rabbit anti Goat IgG (Fc):FITC | ||||||
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
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| Description | Rabbit anti Goat IgG (Fc):HRP | ||||||
References for IgM antibody
-
Williams, A.R. et al. (2017) Dietary cinnamaldehyde enhances acquisition of specific antibodies following helminth infection in pigs.
Vet Immunol Immunopathol. 189: 43-52. -
Tiurbe, G. et al. (2009) Inhibitory effects of rat bone marrow-derived dendritic cells on naïve and alloantigen-specific CD4+ T cells: a comparison between dendritic cells generated with GM-CSF plus IL-4 and dendritic cells generated with GM-CSF plus IL-10.
BMC Res Notes. 2: 12. -
Corsaut, L. et al. (2021) Immunogenicity study of a Streptococcus suis. autogenous vaccine in preparturient sows and evaluation of passive maternal immunity in piglets.
BMC Vet Res. 17 (1): 72. -
Forner, R. et al. (2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows.
PLoS One. 16 (5): e0249366.
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