Erythropoietin antibody
Recombinant Pig erythropoietin (PPP021) detected using Rabbit anti Pig erythropoietin (AHP2389) as the capture reagent and biotinylated Rabbit anti Pig erythropoietin (AHP2389B) as the detection reagent followed by Streptavidin:HRP.
Rabbit anti Pig erythropoietin antibody recognizes erythropoietin, a secreted protein belonging to the EPO/TPO family and also known as EPO or hematopoietin.
Erythropoietin is a hormone secreted in adult mammals primarily from the kidney, and to a lesser extent the liver. In the fetus, EPO is secreted from the liver. EPO functions as a regulatory cytokine in the differentiation of erythrocytes.
Erythropoietin is a hormone secreted in adult mammals primarily from the kidney, and to a lesser extent the liver. In the fetus, EPO is secreted from the liver. EPO functions as a regulatory cytokine in the differentiation of erythrocytes.
Product Details
- Target Species
- Pig
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to pig erythropoietin were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant pig erythropoietin
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of despatch
- Shelf Life
- 12 months from date of despatch
More Information
- UniProt
- P49157 Related reagents
- Entrez Gene
- EPO Related reagents
- GO Terms
- GO:0005128 erythropoietin receptor binding
- GO:0005179 hormone activity
- GO:0043249 erythrocyte maturation
- Regulatory
- For research purposes only
Applications of Erythropoietin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.01ug/ml | 0.2ug/ml | |
| ELISA | 0.5ug/ml | 5ug/ml | |
| Western Blotting | 0.1ug/ml | 2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This product may be used as a capture antibody in a sandwich ELISA together with AHP2389B as the detection reagent. Recombinant Pig erythropoietin (PPP021) may be used as a standard.
- ELISA
- This product may be used as a capture antibody in a sandwich ELISA together with AHP2389B as the detection reagent. Recombinant Pig erythropoietin (PPP021) may be used as a standard.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Product Specific References
Further Reading
-
David, R.B. et al. (2001) The porcine erythropoietin gene: cDNA sequence, genomic sequence and expression analyses in piglets.
Domest Anim Endocrinol. 20 (2): 137-47.
