Lambda Light Chain antibody
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Goat anti Mouse Lambda Light Chain:Alk. Phos.
Goat anti Mouse Lambda Light Chain:Biotin
Goat anti Mouse Lambda Light Chain:FITC
Goat anti Mouse Lambda Light Chain:HRP
Goat anti Mouse Lambda Light Chain
Goat anti Mouse Lambda Light Chain:TRITC
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| 106004 | E | 1 ml |
|
|
| 106008 | E | 1 mg |
|
|
| 106002 | F IF | 1 mg |
|
|
| 106005 | E | 1 ml |
|
|
| 106001 | E IB IF WB | 1 mg | ||
| 106003 | F IF | 0.5 mg |
|
Goat anti Mouse Lambda Light Chain antibody recognizes mouse lambda 1 and lambda 2 light chains (free and bound) as demonstrated by ELISA. To minimise cross reactivity this product has been cross absorbed against mouse paraproteins with kappa light chains.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alkaline Phosphatase - liquid
- Product Form
- Ig Fraction conjugated to Biotin - liquid
- Product Form
- Ig Fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Ig Fraction conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- IgG conjugated to Rhodamine (TRITC) - liquid
- Antiserum Preparation
- Antisera to mouse lambda light chains were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on pooled mouse Igs with lambda chains covalently linked to agarose.
- Buffer Solution
- TRIS buffered saline, 1mM MgCl2
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Borate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
50% Glycerol - Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Preservative Stabilisers
- 50% glycerol
- Preservative Stabilisers
- None Present
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Mouse paraproteins with lambda 1 and lambda 2 light chains.
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Approx. Protein Concentrations
- Ig concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. - Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- UniProt
- P01843
- P01844
- Entrez Gene
- LOC433053
- Iglc2
- GO Terms
- GO:0003823 antigen binding
- GO:0009897 external side of plasma membrane
- GO:0019815 B cell receptor complex
- Regulatory
- For research purposes only
Applications of Lambda Light Chain antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/2000 | 1/4000 | |
| ELISA | 1/1000 | 1/2000 | |
| Flow Cytometry | |||
| Immunofluorescence | |||
| ELISA | 1/4000 | 1/8000 | |
| ELISA | 1/4000 | 1/8000 | |
| Immunoblotting | |||
| Immunofluorescence | |||
| Western Blotting | |||
| Flow Cytometry | |||
| Immunofluorescence |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for Lambda Light Chain antibody
-
Rops, A.L. et al. (2010) The tetraspanin CD37 protects against glomerular IgA deposition and renal pathology.
Am J Pathol. 176 (5): 2188-97. -
Lewis, A. et al. (2017) Recovery from desensitization of IgE-dependent responses in human lung mast cells.
Clin Exp Allergy. Feb 25. [Epub ahead of print]
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