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Lambda Light Chain antibody

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Goat anti Mouse Lambda Light Chain:Alk. Phos.

Goat anti Mouse Lambda Light Chain:Biotin

Goat anti Mouse Lambda Light Chain:FITC

Goat anti Mouse Lambda Light Chain:HRP

Goat anti Mouse Lambda Light Chain

Goat anti Mouse Lambda Light Chain:TRITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
106004
Datasheet
E
MSDS
1 ml loader

106008
Datasheet
E
MSDS
1 mg loader

106002
Datasheet
F IF
MSDS
1 mg loader

106005
Datasheet
E
MSDS
1 ml loader

106001
Datasheet
E IB IF WB
MSDS
1 mg loader

106003
Datasheet
F IF
MSDS
0.5 mg loader

Goat anti Mouse Lambda Light Chain antibody recognizes mouse lambda 1 and lambda 2 light chains (free and bound) as demonstrated by ELISA. To minimise cross reactivity this product has been cross absorbed against mouse paraproteins with kappa light chains.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Product Form
Ig Fraction conjugated to Biotin - liquid
Product Form
Ig Fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Ig Fraction conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Product Form
IgG conjugated to Rhodamine (TRITC) - liquid
Antiserum Preparation
Antisera to mouse lambda light chains were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on pooled mouse Igs with lambda chains covalently linked to agarose.
Buffer Solution
TRIS buffered saline, 1mM MgCl2
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Borate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide (NaN3)
50% Glycerol
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Preservative Stabilisers
50% glycerol
Preservative Stabilisers
None Present
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Mouse paraproteins with lambda 1 and lambda 2 light chains.
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml
Approx. Protein Concentrations
Ig concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
Guaranteed until date of expiry. Please see product label.
Guarantee
Guaranteed until date of expiry. Please see product label.
Guarantee
Guaranteed until date of expiry. Please see product label.
Guarantee
Guaranteed until date of expiry. Please see product label.
Guarantee
Guaranteed until date of expiry. Please see product label.
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

UniProt
P01843
P01844
Entrez Gene
LOC433053
Iglc2
GO Terms
GO:0003823 antigen binding
GO:0009897 external side of plasma membrane
GO:0019815 B cell receptor complex
Regulatory
For research purposes only

Applications of Lambda Light Chain antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000 1/4000
ELISA 1/1000 1/2000
Flow Cytometry
Immunofluorescence
ELISA 1/4000 1/8000
ELISA 1/4000 1/8000
Immunoblotting
Immunofluorescence
Western Blotting
Flow Cytometry
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
TMB Signal+ BUF054A E 100 ml
TMB Core BUF056A E 100 ml
TMB Core+ BUF062A E 100 ml

Product Specific References

References for Lambda Light Chain antibody

  1. Rops, A.L. et al. (2010) The tetraspanin CD37 protects against glomerular IgA deposition and renal pathology.
    Am J Pathol. 176 (5): 2188-97.
  2. Lewis, A. et al. (2017) Recovery from desensitization of IgE-dependent responses in human lung mast cells.
    Clin Exp Allergy. Feb 25. [Epub ahead of print]
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