Kappa Light Chain antibody
Goat anti Mouse kappa light chain antibody (105001) used as a coating reagent in ELISA for determination of mouse antibody levels in serum samples.
Image caption:
Inflammation after immunization with unmodified mRNA is associated with dampened adaptive immune responses.
Blood was collected for analysis of HA specific antibody 21 days (B) and 42 days (C) after study start; 42 days after the study, spleens were collected and assessed for HA specific T cells by ELISPOT (D).
From: Wang Z, Jacobus EJ, Stirling DC, Krumm S, Flight KE, Cunliffe RF, Mottl J, Singh C, Mosscrop LG, Santiago LA, Vogel AB, Kariko K, Sahin U, Erbar S, Tregoning JS.
Reducing cell intrinsic immunity to mRNA vaccine alters adaptive immune responses in mice.
Mol Ther Nucleic Acids. 2023 Oct 5;34:102045.
doi: 10.1016/j.omtn.2023.102045.
This image is from an open access article distributed under terms of a Creative Commons Attribution License
Filter by Application:
E ResetGoat anti Mouse Kappa Light Chain
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Kappa Light Chain
Antibody Quality and Performance Guarantee
| Goat anti mouse kappa light chain polyclonal antibody is specific for mouse kappa light chains (free and bound). This antibody is cross-adsorbed against mouse lambda light chains. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to mouse kappa light chain was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Mouse paraproteins with kappa light chains.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| Flow Cytometry | |
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| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Rabbit anti Goat IgG (Fc):FITC | ||||||
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Rabbit anti Goat IgG (Fc):HRP | ||||||
References for Kappa Light Chain antibody
-
Blakney, A.K. et al. (2021) Innate Inhibiting Proteins Enhance Expression and Immunogenicity of Self-Amplifying RNA.
Mol Ther. 29 (3): 1174-85. -
Wang, Z. et al. (2023) Reducing cell intrinsic immunity to mRNA vaccine alters adaptive immune responses in mice.
Mol Ther Nucleic Acids. 34: 102045.
- UniProt
- P01837
- Entrez Gene
- Igk-C
- GO Terms
- GO:0003823 antigen binding
View more products with KAPPA LIGHT CHAIN specificity
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