IL-2 antibody

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Rabbit anti Mouse Interleukin-2:Biotin

Rabbit anti Mouse Interleukin-2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAM35B E WB 50 µg
AAM35 E FN WB 0.1 mg
Rabbit anti Mouse Interleukin-2 antibody recognizes mouse Interleukin-2 (IL-2), a potent lymphoid cell growth factor which primarily acts on T lymphocytes, in response to antigenic or mitogenic stimulation.

IL-2 stimulates the growth/differentiation of monocytes, macrophages, B lymphocytes, NK cells, and lymphokine activated killer cells which are used in experimental cancer therapy.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Biotin - lyophilised
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.5 ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Reconstitution
Reconstitute with 0.1 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to mouse IL-2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Preservative Stabilisers
None present
Immunogen
Recombinant mouse IL-2 (PMP38)
Approx. Protein Concentrations
IgG concentration 0.1mg/ml after reconstitution
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P04351 Related reagents
Entrez Gene
Il2 Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005134 interleukin-2 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0042104 positive regulation of activated T cell proliferation
GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0046013 regulation of T cell homeostatic proliferation
GO:0048304 positive regulation of isotype switching to IgG isotypes
GO:0050672 negative regulation of lymphocyte proliferation
GO:0050728 negative regulation of inflammatory response
GO:0051024 positive regulation of immunoglobulin secretion
Regulatory
For research purposes only

Applications of IL-2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 1.0ug/ml
Western Blotting 0.1 0.2ug/ml
ELISA 0.5 2.0ug/ml
Functional Assays 0.04 0.05ug/ml
Western Blotting 0.1 0.2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
ELISA
AAM35B may be used in a direct ELISA or as a detection antibody in a sandwich ELISA with AAM35 as the capture reagent and PMP38 as the standard.
ELISA
AAM35 may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA with AAM35B as the detection reagent and PMP38 as the standard.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for IL-2 antibody

  1. Smith, K.A. (1998) Interleukin-2: inception, impact, and implications.
    Science. 240: 1169-1176.