IL-2

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  • Recombinant Mouse Interleukin-2
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PMP38E, FN, WBdatasheet pdfdatasheet pdf20 µg
    PMP38
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Interleukin-2 (IL-2) is a potent lymphoid cell growth factor which primarily acts on T-cells.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified recombinant protein - lyophilised
    • Reconstitution
      Reconstitute with 0.2ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Recombinant protein expressed in E.coli
    • Preservative Stabilisers
      None present
    • Activity
      5 x 106 Units/mg
    • Purity
      >98% by SDS PAGE and HPLC
    • Approx. Protein Concentrations
      0.1 mg/ml after reconstitution.
    • Protein Molecular Weight
      17.2kD (149 amino acid sequence)
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      <0.1 ng/ug
    • Test Principle
    • Buffer Solution
      10mM Sodium Citrate pH4.0
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution.
    • GO Terms
      cytokine activity
      positive regulation of tyrosine phosphorylation of Stat5 protein
      negative regulation of lymphocyte proliferation
      extracellular space
      positive regulation of transcription from RNA polymerase II promoter
      interleukin-2 receptor binding
      immune response
      positive regulation of activated T cell proliferation
      positive regulation of isotype switching to IgG isotypes
      growth factor activity
      regulation of T cell homeostatic proliferation
      negative regulation of inflammatory response
      positive regulation of immunoglobulin secretion
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.20.4 ng/well
      Functional Assays0.11.0 ng/ml
      Western Blotting1.53.0 ng/lane

    • Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate positive/negative controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      This product may be used as a standard in ELISA applications either AAM35 or AAM35B.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      This product may be used as the positive control in Western Blotting applications either AAM35 or AAM35B.
    • Instructions For Use

    Additional IL-2 Formats

    Formats Applications Sizes available
    IL-2 : Rec. Protein E, FN, WB 20 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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