IL-12 antibody

Goat anti Mouse Interleukin-12

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IL-12

Product Code Applications Pack Size List Price Your Price Qty
AAM33
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Goat anti Mouse Interleukin-12 antibody recognizes mouse interleukin-12 (IL-12).

Interleukin-12 is produced by activated B lymphocytes and macrophages. The properties of IL-12 include the stimulation of growth of activated T lymphocytes and the development of TH1 CD4 +ve cells and CD8 +ve cytotoxic T cells.

Goat anti Mouse Interleukin-12 antibody neutralizes murine IL-12 activity with an ND50 for mIL-12 (2ngml-1 of between 0.002 and 0.002μg ml-1.

Target Species
Mouse
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to mouse IL-12 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant mouse IL-12.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0 ug/ml
Functional Assays 2.0 3.0 ng/ml
Western Blotting 0.1 0.2 ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
ELISA
This purified mouse IL-12 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with a biotinylated mouse IL-12 antibody (AAM33B) as the detection reagent.
Western Blotting
This purified mouse IL-12 antibody may be used in Western Blotting applications under either reducing or non-reducing conditions.

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RRID
AB_323697
UniProt
P43432
P43431
Entrez Gene
Il12a
Il12b
GO Terms
GO:0005125 cytokine activity
GO:0005143 interleukin-12 receptor binding
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0032729 positive regulation of interferon-gamma production
GO:0042102 positive regulation of T cell proliferation
GO:0042163 interleukin-12 beta subunit binding
GO:0042832 defense response to protozoan
GO:0043514 interleukin-12 complex
GO:0001916 positive regulation of T cell mediated cytotoxicity
GO:0002230 positive regulation of defense response to virus by host
GO:0002827 positive regulation of T-helper 1 type immune response
GO:0002862 negative regulation of inflammatory response to antigenic stimulus
GO:0016020 membrane
GO:0004896 cytokine receptor activity
GO:0044130 negative regulation of growth of symbiont in host
GO:0042093 T-helper cell differentiation
GO:0042104 positive regulation of activated T cell proliferation
GO:0042164 interleukin-12 alpha subunit binding
GO:0042803 protein homodimerization activity
View more products with IL-12 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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