IL-12 antibody

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Goat anti Mouse Interleukin-12:Biotin

Goat anti Mouse Interleukin-12

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAM33B E WB 50 µg
AAM33 E FN WB 0.1 mg
Goat anti Mouse Interleukin-12 antibody recognizes mouse interleukin-12 (IL-12).

Interleukin-12 is produced by activated B lymphocytes and macrophages. The properties of IL-12 include the stimulation of growth of activated T lymphocytes and the development of TH1 CD4 +ve cells and CD8 +ve cytotoxic T cells.

Goat anti Mouse Interleukin-12 antibody neutralizes murine IL-12 activity with an ND50 for mIL-12 (2ngml-1 of between 0.002 and 0.002μg ml-1.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Biotin - lyophilised
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add sodium azide
Antiserum Preparation
Antisera to mouse IL-12 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Preservative Stabilisers
None present
Immunogen
Recombinant mouse IL-12 (PMP40)
Approx. Protein Concentrations
IgG concentration 0.1mg/ml after reconstitution.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P43432 Related reagents
P43431 Related reagents
Entrez Gene
Il12a Related reagents
Il12b Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005143 interleukin-12 receptor binding
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0032729 positive regulation of interferon-gamma production
GO:0042102 positive regulation of T cell proliferation
GO:0042163 interleukin-12 beta subunit binding
GO:0042832 defense response to protozoan
GO:0043514 interleukin-12 complex
GO:0001916 positive regulation of T cell mediated cytotoxicity
GO:0002230 positive regulation of defense response to virus by host
GO:0002827 positive regulation of T-helper 1 type immune response
GO:0002862 negative regulation of inflammatory response to antigenic stimulus
GO:0016020 membrane
GO:0004896 cytokine receptor activity
GO:0044130 negative regulation of growth of symbiont in host
GO:0042093 T-helper cell differentiation
GO:0042104 positive regulation of activated T cell proliferation
GO:0042164 interleukin-12 alpha subunit binding
GO:0042803 protein homodimerization activity
Regulatory
For research purposes only

Applications of IL-12 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 1.0ug/ml
Western Blotting 0.1 0.2ug/ml
ELISA 0.5 2.0 ug/ml
Functional Assays 2.0 3.0 ng/ml
Western Blotting 0.1 0.2 ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
ELISA
This biotinylated mouse IL-12 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with a purified mouse IL-12 antibody (AAM33) as the capture reagent and recombinant mouse IL-12 (PMP40) as the standard.
ELISA
This purified mouse IL-12 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with a biotinylated mouse IL-12 antibody (AAM33B) as the detection reagent and recombinant mouse IL-12 (PMP40) as the standard.
Western Blotting
This biotinylated mouse IL-12 antibody may used in Western Blotting application under either reducing of non-reducing conditions with recombinant mouse IL-12 (PMP40) as the positive control.
Western Blotting
This purified mouse IL-12 antibody may used in Western Blotting applications under either reducing or non-reducing conditions with recombinant mouse IL-12 (PMP40) as the positive control.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB