IgG antibody

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Goat ANTI-MOUSE IgG StarBright™ Blue 700

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
12004159 WB 80 µl
12004158 WB 400 µl
StarBright Blue 700 Secondary Antibodies are ideal for fluorescent western blotting — either for the detection of a single target protein or for multiplex detection of several proteins on one blot, without stripping and reprobing. The StarBright Fluorophore is composed of a condensed polymer made up of multiple light-absorbing and -emitting monomers which provides an exceptionally bright signal (high quantum yield) compared to most traditional fluorophores.

StarBright Blue 700 Fluorescent Secondary Antibodies can be used with traditional fluorophores like the DyLight 800 Fluorophore for multiplexing. In addition, StarBright Antibodies can be used with Bio-Rad's stain-free technology and/or the hFAB™ Rhodamine Anti-Housekeeping Primary Antibodies for protein normalization. These antibodies are optimized for use with the ChemiDoc™ MP Imaging System, permitting detection of up to three proteins in a single blot.

Features and Benefits

Easy multiplexing — StarBright Secondary Antibodies can be used with IRDye 800 or other common fluorophores to detect multiple proteins on the same blot
Short exposure time — the exceptional brightness of the StarBright Fluorophore leads to shorter exposure times compared to traditional fluorescent dyes: seconds vs. minutes
High signal-to-noise ratio — StarBright Secondary Antibodies emit in the red/far red, where there is minimal background (Exmax/Emmax = 470 nm/700 nm)
Low nonspecific binding — StarBright Fluorophore is conjugated to highly cross-adsorbed antibodies

Goat Anti-Mouse IgG StarBright Blue 700 has been cross-adsorbed against bovine, goat, human, rabbit and rat.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to StarBright Blue 700 - lyophilized
Reconstitution
Resuspend contents of the tube in the indicated volume of distilled or deionized water and leave on ice for at least 30 min prior to use. Brief centrufugation (pulse spin for 2-3 sec at max speed in a tabletop microcentrifuge) may be used to collect the contents at the bottom of the tube. Do not centrifuge more than 10 sec.
Antiserum Preparation
Antiserum to Mouse IgG was raised by repeated immunisation of Goats with highly purified antigen.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
>80% Sucrose
<1% Polyethylene Glycol
<0.04% 2-Methyl-2H-Isothiazol-3-One
<10% Bovine Serum Albumin

Storage Information

Storage
1 year at -20oC lyophilized; 6 months at +4oC after resuspension.
This product is photosensitive and should be protected from light
DO NOT FREEZE the solubilized material
Shelf Life
Please see label for expiry date

More Information

Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/2,500 1/5,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
StarBright Blue 700 Secondary Antibodies can be used for multiplex fluorescent western blotting with the right combination of fluorophores. These fluorescent secondary antibodies can also be used to detect post-translational modifications like phosphorylation of a target protein.
Note: StarBright Antibodies are not suitable for stripping and reprobing.
Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/12004157.pdf

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Goat ANTI-RABBIT IgG StarBright™ Blue 700 12004161 400 µl WB
Goat ANTI-RABBIT IgG StarBright™ Blue 700 12004162 80 µl WB
ANTI-ACTIN hFAB™ Rhodamine Antibody 12004163 200 µl WB
ANTI-ACTIN hFAB™ Rhodamine Antibody 12004164 40 µl WB
ANTI-TUBULIN hFAB™ Rhodamine Antibody 12004165 200 µl WB
ANTI-TUBULIN hFAB™ Rhodamine Antibody 12004166 40 µl WB
ANTI-GAPDH hFAB™ Rhodamine Antibody 12004167 200 µl WB
ANTI-GAPDH hFAB™ Rhodamine Antibody 12004168 40 µl WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB