Actin antibody | AbD22606
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WB ResetANTI-ACTIN hFAB™ Rhodamine Antibody
- Product Type
- Monoclonal Antibody
- Clone
- AbD22606
- Isotype
- HuCAL Fab monovalent
- Specificity
- Actin
hFAB Rhodamine Housekeeping Protein Fluorescent Primary Antibodies allow one-step detection of common housekeeping proteins like actin, tubulin, and GAPDH in your samples. These anti-actin, anti-tubulin, and anti-GAPDH antibodies are directly labeled with a rhodamine derivative (Exmax/Emmax = 530 nm/580 nm). The hFAB Rhodamine Antibodies were generated with Human Combinatorial Antibody Library (HuCAL®) Technology, providing exceptional specificity. These housekeeping protein antibodies can be used for protein normalization when the protein loading amounts are in the linear dynamic range. hFAB Rhodamine Antibodies can be imaged with the ChemiDoc MP Imaging System, and can be used in multiplex experiments with StarBright Blue 700 Secondary Antibodies as well as other traditional fluorescent antibodies, permitting detection of multiple target proteins and the housekeeping protein in a single blot. Features and Benefits Simple — No requirement for a secondary antibody; one-step detection of housekeeping proteins using highly cross-adsorbed antibodies for high specificity and sensitivity Versatile — Compatible with use of a wide range of other fluorophores including StarBright Blue 700 and DyLight 800 Fluorophores for multiplex western blotting Unique — HuCAL Technology eliminates cross-reactivity with primary/secondary antibodies from other species Diverse — Antibodies have been tested to detect human or mouse housekeeping proteins |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
-
12004164
A monovalent human recombinant Fab selected from the HuCAL phage display library, expressed in E. coli. and purified using NiNTA affinity chromatography. This antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain and conjugated to rhodamine - lyophilized -
12004163
A monovalent human recombinant Fab selected from the HuCAL phage display library, expressed in E. coli. and purified using NiNTA affinity chromatography. This antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain and conjugated to rhodamine - lyophilized - Reconstitution
- Resuspend contents of the tube in the indicated volume of distilled or deionized water and leave on ice for at least 30 min prior to use. Brief centrufugation (pulse spin for 2-3 sec at maximum speed on a tabletop microcentrifuge) may be used to collect the contents at the bottom of the tube.
- Buffer Solution
- 12004164: Phosphate buffered saline.
- 12004163: Phosphate buffered saline
- Preservative Stabilisers
- >80% Sucrose
<0.04% 2-Methyl-2H-Isothiazol-3-One
<10% bovine serum albumin - Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Rhodamine 530 580 - Regulatory
- For research purposes only
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
This product is photosensitive and should be protected from light
DO NOT FREEZE the solubilized material
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Western Blotting | 1/1,000 | 1/10,000 |
- Instructions For Use
-
12004164, 12004163
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/12004163.pdf
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for Actin antibody
-
Seo, T. et al. (2023) MARCH family E3 ubiquitin ligases selectively target and degrade cadherin family proteins.
bioRxiv. Aug 10 [Epub ahead of print]. -
Ishiyama, R. et al. (2024) Production of infectious reporter murine norovirus by VP2 trans-complementation.
J Virol. : e0126123. -
Garg, S. & , Miousse, I.R. (2024) Rescue of Methionine Dependence by Cobalamin in a Human Colorectal Cancer Cell Line
Nutrients. 16 (7): 997. -
Hyder, U. et al. (2024) KAP1 negatively regulates RNA polymerase II elongation kinetics to activate signal-induced transcription
bioRχiv 5 May [Preprint] -
Todorova, M.N. et al. (2024) Punica granatum L. leaf extract enhances stress tolerance and promotes healthy longevity through HLH-30/TFEB, DAF16/FOXO, and SKN1/NRF2 crosstalk in Caenorhabditis elegans.
Phytomedicine. 134: 155971. -
Smith-Davidson, P. et al. (2024) Prostaglandin E Receptor 2 (EP2) Dysregulation in Allergic Fungal Rhinosinusitis Nasal Polyp Epithelium.
Laryngoscope. Nov 02 [Epub ahead of print]. -
Le, K.P.N. et al. (2024) Endogenous ZAP is associated with altered Zika virus infection phenotype
Virology Journal. 21: 285.
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