EGF antibody

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Goat anti Mouse EGF

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAM52 E WB 0.1 mg
Goat anti Mouse EGF antibody recognizes mouse Epidemal Growth Factor (EGF)

EGF is expressed by a range of cells and functions in the stimulation and control of the proliferation of epidermal and epithelial cells. EGF is also chemotactic for fibroblasts and epithelial cells.

Product Details

Target Species
Mouse
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.1 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Antiserum Preparation
Antisera to mouse EGF were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant mouse EGF.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. For long term storage the addition of 0.09% sodium azide is recommended.

N.B. For functional studies do not add azide.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P01132 Related reagents
Entrez Gene
Egf Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0000186 activation of MAPKK activity
GO:0005509 calcium ion binding
GO:0016021 integral to membrane
GO:0005154 epidermal growth factor receptor binding
GO:0005615 extracellular space
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0008083 growth factor activity
GO:0018108 peptidyl-tyrosine phosphorylation
GO:0021940 positive regulation of cerebellar granule cell precursor proliferation
GO:0048754 branching morphogenesis of a tube
GO:0050730 regulation of peptidyl-tyrosine phosphorylation
GO:0060749 mammary gland alveolus development
Regulatory
For research purposes only

Applications of EGF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5ug/ml
Western Blotting 0.1ug/ml 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB