CD197 antibody

Goat anti Mouse CD197

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CD197

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AAM70
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E F FN P WB 50 µg loader
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Goat anti Mouse CD197 antibody recognizes mouse C-C chemokine receptor type 7, also known as CD197, CCR7, Epstein-Barr virus-induced G-protein coupled receptor 1 or MIP-3 beta receptor. CD197 is a 378 amino acid ~43 kDa multi pass type III transmembrane glycoprotein and member of the G-protein coupled receptor 1 family (UniProt: P74447). CD197 is expressed by various lymphoid tissues, activated B and T cells, naive T cells, NK cells and dendritic cells (DCs), which acts a high-affinity receptor for the chemokines MIP-3beta (CCL19) and CCL21.

Ligand binding of CD197 plays a vital role in the homing of naive T cells and antigen-loaded DCs into secondary organs. Expression of CD197 is strongly up-regulated in B cells infected with Epstein-Barr virus (EBV) and T cells infected with herpesvirus 6 or 7. Goat anti Mouse CD197 antibody can be used to distinguish between central memory and effector memory T cells and is suitable for use in immunocytochemistry.

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to mouse CD197 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide, 0.1% Bovine Serum Albumin.
Immunogen
Synthetic peptide DPGKPRKNVLVVALLVIFQVC corresponding to amino acids 2-22 of mouse CD197.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100000
Flow Cytometry 1/10
Functional Assays 8ug
Immunohistology - Paraffin 1/250
Western Blotting 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
ELISA
This product is suitable for use in indirect ELISA applications.
Histology Positive Control Tissue
Spleen.

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Synonyms
CCR7
RRID
AB_2228802
UniProt
P47774
P32248
Entrez Gene
CCR7
Ccr7
GO Terms
GO:0005887 integral to plasma membrane
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0007204 elevation of cytosolic calcium ion concentration
GO:0016021 integral to membrane
GO:0006955 immune response
GO:0009897 external side of plasma membrane
GO:0016493 C-C chemokine receptor activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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