CD197 antibody


Ligand binding of CD197 plays a vital role in the homing of naive T cells and antigen-loaded DCs into secondary organs. Expression of CD197 is strongly up-regulated in B cells infected with Epstein-Barr virus (EBV) and T cells infected with herpesvirus 6 or 7. Goat anti Mouse CD197 antibody can be used in research studies to distinguish between central memory and effector memory T cells and is reported as suitable for use in immunocytochemistry.
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to mouse CD197 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide, 0.1% Bovine Serum Albumin.
- Immunogen
- Synthetic peptide DPGKPRKNVLVVALLVIFQVC corresponding to amino acids 2-22 of mouse CD197.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P47774
- P32248
- Entrez Gene
- CCR7
- Ccr7
- GO Terms
- GO:0005887 integral to plasma membrane
- GO:0006935 chemotaxis
- GO:0006954 inflammatory response
- GO:0007204 elevation of cytosolic calcium ion concentration
- GO:0016021 integral to membrane
- GO:0006955 immune response
- GO:0009897 external side of plasma membrane
- GO:0016493 C-C chemokine receptor activity
- Regulatory
- For research purposes only
Applications of CD197 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/100000 | ||
Flow Cytometry | 1/10 | ||
Functional Assays | 8ug | ||
Immunohistology - Paraffin | 1/250 | ||
Western Blotting | 1/1000 |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- ELISA
- This product is suitable for use in indirect ELISA applications.
- Histology Positive Control Tissue
- Spleen.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
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Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml |
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