Leumorphin antibody

Rabbit anti Leumorphin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Leumorphin

Product Code Applications Pack Size List Price Your Price Qty
AHP378
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SDS Safety Datasheet SDS
C 0.1 ml loader
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Rabbit anti Leumorphin antibody recognizes rat leumorphin, also known as dynorphin B29. Leumorphin is a 28 amino acid opioid peptide resulting from cleavage of proenkephalin-B. Leumorphin positive cells are found in the paraventricular, supraoptic nucleus and fibres in the lateral hypothalamus of colchicine treated rat brain (Neal & Newman 1989). Leumorphin has been reported to exert anti-apoptotic effects in rat PC12 pheochromocytoma cells (Lee et al. 2005).

Rabbit anti leumorphin has been used successfully to detect leumorphin in murine brain using immunohistochemistry on cryostat sections.(Zhang et al.2005).

Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to Leumorphin were raised by repeated immunisation of rabbits with highly purified antigen. Whole serum is supplied - diluted.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Leumorphin: Arg-Ser-Gln-Glu-Asn-Pro-Asn-Thr-Tyr-Ser-Glu-Asp-Leu-Asp-Val (rat 14-28)
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/100 1/2000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for Leumorphin antibody

  1. Zhang, D. et al. (2005) Repeated cocaine administration induces gene expression changes through the dopamine D1 receptors.
    Neuropsychopharmacology. 30 (8): 1443-54.
  2. Zhang, J. et al. (2006) c-Fos facilitates the acquisition and extinction of cocaine-induced persistent changes.
    J Neurosci. 26: 13287-96.
  3. Ruiz-De Diego, I. et al. (2015) Activation of DREAM (downstream regulatory element antagonistic modulator), a calcium-binding protein, reduces L-DOPA-induced dyskinesias in mice.
    Biol Psychiatry. 77 (2): 95-105.
  4. Hernández, L.F. et al. (2017) Striatal activation by optogenetics induces dyskinesias in the 6-hydroxydopamine rat model of Parkinson disease.
    Mov Disord. 32 (4): 530-537.

Further Reading

  1. NealCr, J.r. & Newman, S.W. (1989) Prodynorphin peptide distribution in the forebrain of the Syrian hamster and rat: a comparative study with antisera against dynorphin A, dynorphin B, and the C-terminus of the prodynorphin precursor molecule.
    J Comp Neurol. 288 (3): 353-86.
  2. Xu, M. et al. (1994) Dopamine D1 receptor mutant mice are deficient in striatal expression of dynorphin and in dopamine-mediated behavioral responses.
    Cell. 79 (4): 729-42.
  3. Drake, C.T. et al. (1994) Dynorphin opioids present in dentate granule cells may function as retrograde inhibitory neurotransmitters.
    J Neurosci. 14 (6): 3736-50.

Immunofluorescence

Immunohistology - Frozen

Synonyms
Dynorphin B-29
RRID
AB_2283688
UniProt
P06300
Entrez Gene
Pdyn
GO Terms
GO:0001515 opioid peptide activity
GO:0005576 extracellular region
GO:0007218 neuropeptide signaling pathway
GO:0007268 synaptic transmission
GO:0046928 regulation of neurotransmitter secretion
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"