Leumorphin antibody

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Rabbit anti Leumorphin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP378 C 0.1 ml
Rabbit anti Leumorphin antibody recognizes rat leumorphin, also known as dynorphin B29. Leumorphin is a 28 amino acid opioid peptide resulting from cleavage of proenkephalin-B. Leumorphin positive cells are found in the paraventricular, supraoptic nucleus and fibres in the lateral hypothalamus of colchicine treated rat brain (Neal & Newman 1989). Leumorphin has been reported to exert anti-apoptotic effects in rat PC12 pheochromocytoma cells (Lee et al. 2005).

Rabbit anti leumorphin has been used successfully to detect leumorphin in murine brain using immunohistochemistry on cryostat sections.(Zhang et al.2005).

Product Details

Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to Leumorphin were raised by repeated immunisation of rabbits with highly purified antigen. Whole serum is supplied - diluted.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Leumorphin: Arg-Ser-Gln-Glu-Asn-Pro-Asn-Thr-Tyr-Ser-Glu-Asp-Leu-Asp-Val (rat 14-28)

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P06300 Related reagents
Entrez Gene
Pdyn Related reagents
GO Terms
GO:0001515 opioid peptide activity
GO:0005576 extracellular region
GO:0007218 neuropeptide signaling pathway
GO:0007268 synaptic transmission
GO:0046928 regulation of neurotransmitter secretion
Regulatory
For research purposes only

Applications of Leumorphin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/100 1/2000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Application Based External Images

Immunofluorescence

Immunohistology - Frozen

Product Specific References

References for Leumorphin antibody

  1. Drake, C.T. et al. (1994) Dynorphin opioids present in dentate granule cells may function as retrograde inhibitory neurotransmitters.
    J Neurosci. 14 (6): 3736-50.
  2. Xu, M. et al. (1994) Dopamine D1 receptor mutant mice are deficient in striatal expression of dynorphin and in dopamine-mediated behavioral responses.
    Cell. 79 (4): 729-42.
  3. NealCr, J.r. & Newman, S.W. (1989) Prodynorphin peptide distribution in the forebrain of the Syrian hamster and rat: a comparative study with antisera against dynorphin A, dynorphin B, and the C-terminus of the prodynorphin precursor molecule.
    J Comp Neurol. 288 (3): 353-86.
  4. Zhang, D. et al. (2005) Repeated cocaine administration induces gene expression changes through the dopamine D1 receptors.
    Neuropsychopharmacology. 30 (8): 1443-54.
  5. Zhang, J. et al. (2006) c-Fos facilitates the acquisition and extinction of cocaine-induced persistent changes.
    J Neurosci. 26: 13287-96.
  6. Ruiz-De Diego, I. et al. (2015) Activation of DREAM (downstream regulatory element antagonistic modulator), a calcium-binding protein, reduces L-DOPA-induced dyskinesias in mice.
    Biol Psychiatry. 77 (2): 95-105.
  7. FHernández, L. et al. (2017) Striatal activation by optogenetics induces dyskinesias in the 6-hydroxydopamine rat model of Parkinson disease.
    Mov Disord. 32 (4): 530-537.