Rabbit anti Leumorphin antibody
recognizes rat leumorphin, also known as dynorphin B29. Leumorphin is a 28 amino acid opioid peptide resulting from cleavage of proenkephalin-B. Leumorphin positive cells are found in the paraventricular, supraoptic nucleus and fibres in the lateral hypothalamus of colchicine treated rat brain (Neal & Newman 1989
). Leumorphin has been reported to exert anti-apoptotic effects in rat PC12
pheochromocytoma cells (Lee et al. 2005
Rabbit anti leumorphin has been used successfully to detect leumorphin in murine brain using immunohistochemistry on cryostat sections.(Zhang et al.2005
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to Leumorphin were raised by repeated immunisation of rabbits with highly purified antigen. Whole serum is supplied - diluted.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
|1%||Bovine Serum Albumin|
- Leumorphin: Arg-Ser-Gln-Glu-Asn-Pro-Asn-Thr-Tyr-Ser-Glu-Asp-Leu-Asp-Val (rat 14-28)
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
opioid peptide activity
neuropeptide signaling pathway
regulation of neurotransmitter secretion
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Leumorphin antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Application Based External Images
Immunohistology - Frozen
Product Specific References
References for Leumorphin antibody
Drake, C.T. et al. (1994) Dynorphin opioids present in dentate granule cells may function as retrograde inhibitory neurotransmitters.
J Neurosci. 14 (6): 3736-50.
Xu, M. et al. (1994) Dopamine D1 receptor mutant mice are deficient in striatal expression of dynorphin and in dopamine-mediated behavioral responses.
Cell. 79 (4): 729-42.
NealCr, J.r. & Newman, S.W. (1989) Prodynorphin peptide distribution in the forebrain of the Syrian hamster and rat: a comparative study with antisera against dynorphin A, dynorphin B, and the C-terminus of the prodynorphin precursor molecule.
J Comp Neurol. 288 (3): 353-86.
Zhang, D. et al. (2005) Repeated cocaine administration induces gene expression changes through the dopamine D1 receptors.
Neuropsychopharmacology. 30 (8): 1443-54.
Zhang, J. et al. (2006) c-Fos facilitates the acquisition and extinction of cocaine-induced persistent changes.
J Neurosci. 26: 13287-96.
Ruiz-De Diego, I. et al. (2015) Activation of DREAM (downstream regulatory element antagonistic modulator), a calcium-binding protein, reduces L-DOPA-induced dyskinesias in mice.
Biol Psychiatry. 77 (2): 95-105.
FHernández, L. et al. (2017) Striatal activation by optogenetics induces dyskinesias in the 6-hydroxydopamine rat model of Parkinson disease.
Mov Disord. 32 (4): 530-537.