Rabbit anti ZAP 70 (pTyr493) antibody
recognizes ZAP 70, also known as Zeta-chain-associated protein kinase 70 when phosphorylated at tyrosine 493. ZAP70 is a member of the tyrosine kinase family. ZAP 70 plays a key role in the activation of T cells. ZAP 70 is phosphorylated at tyrosine 493 by Lck in response to T cell receptor stimulation (Wang et al. 2010
). Phosphorylation of ZAP 70 at tyrosine 493 fully activates ZAP 70 leading to phosphorylation of downstream targets of the T cell activation pathway.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to ZAP 70 (pTyr493) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding tyrosine 493 of human ZAP 70
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
non-membrane spanning protein tyrosine kinase activity
intracellular protein kinase cascade
T cell receptor complex
positive thymic T cell selection
T cell receptor signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of ZAP 70 (pTyr493) antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available
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