VEGF Receptor 2 antibody
Rabbit anti Human VEGFR2 antibody (AHP1327) used for the identification of VEGF expressing cells in atherosclerotic plaques by immunohistochemistry.
Image caption:
Example of immunostaining of different antibodies.
A: Overview. C: CD68 positive macrophages. E: VEGFR2. F: VEGF. H: Dll4. Black bars represent 1000 μm (A) and 200 μm (B-H).
From: Hjelmgren O, Gellerman K, Kjelldahl J, Lindahl P, Bergström GML (2016)
Increased Vascularization in the Vulnerable Upstream Regions of Both Early and Advanced Human Carotid Atherosclerosis.
PLoS ONE 11(12): e0166918.
Rabbit anti Human VEGF receptor 2 antibody detects vascular endothelial growth factor receptor 2 (VEGFR2), a member of the tyrosine kinase family that plays an essential role in transducing the major signals for angiogenesis. VEGFR2 has also been implicated in embryonic development, wound healing, cell proliferation, migration and differentiation.
VEGFR2 is expressed mostly on vascular endothelial cells, and is expressed on a subpopulation of haematopoietic cells which might be the progenitor for endothelial cells.
Mutated forms of VEGFR2 have been implicated in cancer and diabetic retinopathy, where it results in pathological angiogenesis.
VEGFR2 is expressed mostly on vascular endothelial cells, and is expressed on a subpopulation of haematopoietic cells which might be the progenitor for endothelial cells.
Mutated forms of VEGFR2 have been implicated in cancer and diabetic retinopathy, where it results in pathological angiogenesis.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human VEGF receptor 2 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide (NaN3) - Immunogen
- Synthetic peptide corresponding to an internal domain of human VEGF receptor 2, conjugated to Keyhole Limpet Hemocyanin (KLH).
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P35968
- Entrez Gene
- KDR
- GO Terms
- GO:0001525 angiogenesis
- GO:0001938 positive regulation of endothelial cell proliferation
- GO:0004716 receptor signaling protein tyrosine kinase activity
- GO:0019838 growth factor binding
- GO:0005021 vascular endothelial growth factor receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005178 integrin binding
- GO:0005524 ATP binding
- GO:0006468 protein phosphorylation
- GO:0008360 regulation of cell shape
- GO:0051879 Hsp90 protein binding
- GO:0010595 positive regulation of endothelial cell migration
- GO:0044419 interspecies interaction between organisms
- GO:0050927 positive regulation of positive chemotaxis
- GO:0051894 positive regulation of focal adhesion assembly
- Regulatory
- For research purposes only
Applications of VEGF Receptor 2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 | 10ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Breast carcinoma.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml |
Product Specific References
References for VEGF Receptor 2 antibody
-
Shibuya, M. (2006) Differential roles of vascular endothelial growth factor receptor-1 and receptor-2 in angiogenesis.
J Biochem Mol Biol. 39 (5): 469-78. -
Hjelmgren, O. et al. (2016) Increased Vascularization in the Vulnerable Upstream Regions of Both Early and Advanced Human Carotid Atherosclerosis.
PLoS One. 11 (12): e0166918.
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