Tubulin Alpha 4A Chain antibody

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Rabbit anti Tubulin Alpha 4A Chain

Tubulin alpha 4A chain antibody recognizes the tubulin alpha-4A chain, also known as TUBA4A, tubulin H2-alpha, tubulin alpha-1 chain or tubulin, alpha 1 (testis specific). This antibody has been extensively validated for western blotting using whole cell lysates.

Tubulin Alpha 4A Chain Antibody with Control Lysate

Product Type: PrecisionAb™ Polyclonal
Isotype: Polyclonal IgG
Format: Purified

Product Code	Applications	Pack Size
VPA00231	WB	100 µl
VPA00231KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Rabbit anti Human tubulin alpha 4A chain antibody recognizes tubulin alpha 4A chain, also known as tubulin H2-alpha, tubulin alpha-1 chain, and tubulin, alpha 1 (testis specific).

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. Encoded by the TUBA4A gene, tubulin alpha 4A chain is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for TUBA4A (provided by RefSeq, Jun 2013).

Rabbit anti Human tubulin alpha 4A chain antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti tubulin alpha 4A chain detects a band of approximately 55 kDa in HeLa cell lysates

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Product Form

Purified IgG - liquid

Preparation

Rabbit polyclonal antibody purified by affinity chromatography

Preparation

20μl Rabbit polyclonal antibody purified by affinity chromatography

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Immunogen

KLH conjugated synthetic peptide between 417-446 amino acids from the C-terminal region of human tubulin alpha 4A chain

Instructions For Use

Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg HeLa lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: P68366 Related reagents
Entrez Gene: TUBA4A Related reagents
GO Terms: GO:0000086 G2/M transition of mitotic cell cycle; GO:0005515 protein binding; GO:0003924 GTPase activity; GO:0002576 platelet degranulation; GO:0005576 extracellular region; GO:0005829 cytosol; GO:0005198 structural molecule activity; GO:0005525 GTP binding; GO:0005874 microtubule; GO:0007018 microtubule-based movement; GO:0030168 platelet activation; GO:0051084 'de novo' posttranslational protein folding; GO:0051258 protein polymerization
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of Tubulin Alpha 4A Chain antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Rabbit IgG (H/L):HRP	STAR208P	2 ml	WB
Goat anti Rabbit IgG (H/L):HRP	STAR208P	2 ml	WB

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