TGF Alpha antibody

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Rabbit anti Human TGF Alpha

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP284G E FN P* WB 0.1 mg
Rabbit anti human TGFα recognizes tranforming growth factor alpha. TGFα is a 50 amino acid EGF-related polypeptide expressed in a range of tissues, including skin, pancreas, liver, duodenum, salivary glands, urinary and respiratory systems, and the developing and mature central nervous system (CNS).

TGFα is produced by monocytes, keratinocytes and neuronal cells and stimulates the proliferation of a range of epidermal and epithelial cells, through binding to the epidermal growth factor receptor (EGFR). TGFα is mitogenic for multipotent neural progenitors and is involved in astrogliosis (Junier 2000).

Product Details

Target Species
Human
Product Form
Purified IgG - lyophilised
Reconstitution
Reconstitute with 0.1 ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Antiserum Preparation
Antisera to human TGFα were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was was prepared by antigen affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant human TGFα (PHP037)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

Please see label for expiry date.

More Information

UniProt
P01135 Related reagents
Entrez Gene
TGFA Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0000187 activation of MAPK activity
GO:0016021 integral to membrane
GO:0004708 MAP kinase kinase activity
GO:0004871 signal transducer activity
GO:0005154 epidermal growth factor receptor binding
GO:0045741 positive regulation of epidermal growth factor receptor activity
GO:0005615 extracellular space
GO:0008083 growth factor activity
GO:0008283 cell proliferation
GO:0009986 cell surface
GO:0045840 positive regulation of mitosis
GO:0050679 positive regulation of epithelial cell proliferation
GO:0051781 positive regulation of cell division
Regulatory
For research purposes only

Applications of TGF Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Functional Assays 2.5 3.7ug/ml
Immunohistology - Paraffin 1 0.5ug/ml
Western Blotting 0.1ug/ml 0.2ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
ELISA
This purifed human TGFα antibody may be used in an indirect ELISA with recombinant human TGFα (PHP037) as the standard.
Western Blotting
Rabbit anti human TGFα may used in Western Blotting application under either reducing or non-reducing conditions with recombinant human TGFα (PHP037) as the positive control.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for TGF Alpha antibody

  1. Junier, Marie-Pierre. (2000) What role(s) for TGFalpha in the central nervous system?
    Prog. Neurobiol. 62: 443-473.