TGF Alpha antibody
Rabbit anti human TGFα recognizes tranforming growth factor alpha. TGFα is a 50 amino acid EGF-related polypeptide expressed in a range of tissues, including skin, pancreas, liver, duodenum, salivary glands, urinary and respiratory systems, and the developing and mature central nervous system (CNS).
TGFα is produced by monocytes, keratinocytes and neuronal cells and stimulates the proliferation of a range of epidermal and epithelial cells, through binding to the epidermal growth factor receptor (EGFR). TGFα is mitogenic for multipotent neural progenitors and is involved in astrogliosis (Junier 2000).
TGFα is produced by monocytes, keratinocytes and neuronal cells and stimulates the proliferation of a range of epidermal and epithelial cells, through binding to the epidermal growth factor receptor (EGFR). TGFα is mitogenic for multipotent neural progenitors and is involved in astrogliosis (Junier 2000).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1 ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
- Antiserum Preparation
- Antisera to human TGFα were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was was prepared by antigen affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human TGFα (PHP037)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution
Storage Information
- Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
More Information
- UniProt
- P01135
- Entrez Gene
- TGFA
- GO Terms
- GO:0005886 plasma membrane
- GO:0000187 activation of MAPK activity
- GO:0016021 integral to membrane
- GO:0004708 MAP kinase kinase activity
- GO:0004871 signal transducer activity
- GO:0005154 epidermal growth factor receptor binding
- GO:0045741 positive regulation of epidermal growth factor receptor activity
- GO:0005615 extracellular space
- GO:0008083 growth factor activity
- GO:0008283 cell proliferation
- GO:0009986 cell surface
- GO:0045840 positive regulation of mitosis
- GO:0050679 positive regulation of epithelial cell proliferation
- GO:0051781 positive regulation of cell division
- Regulatory
- For research purposes only
Applications of TGF Alpha antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.5 | 2.0ug/ml | |
| Functional Assays | 2.5 | 3.7ug/ml | |
| Immunohistology - Paraffin 1 | 0.5ug/ml | ||
| Western Blotting | 0.1ug/ml | 0.2ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- ELISA
- This purifed human TGFα antibody may be used in an indirect ELISA with recombinant human TGFα (PHP037) as the standard.
- Western Blotting
- Rabbit anti human TGFα may used in Western Blotting application under either reducing or non-reducing conditions with recombinant human TGFα (PHP037) as the positive control.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
|
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
|
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for TGF Alpha antibody
-
Junier, M.P. (2000) What role(s) for TGFalpha in the central nervous system?
Prog Neurobiol. 62 (5): 443-73.
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