SPT16 antibody

Rabbit anti Human SPT16

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
SPT16

Product Code Applications Pack Size List Price Your Price Qty
AHP3107
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.1 ml loader
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loader
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Rabbit anti Human SPT16 antibody recognizes FACT complex subunit SPT16.

SPT16 is part of the FACT (facilitates chromatin transcription) complex that specifically interacts with histones H2A/H2B to initiate nucleosome disassembly and transcription elongation (Abe et al. 2011). SPT16 has been shown to be essential for nucleosome disruption and assembly on individual rDNA genes. Mutations in SPT16 result in defective nucleosome disruption and inability to fully activate pol I transcription when cells exit the S phase of the cell cycle (Johnson et al. 2013).

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human SPT16 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.035% Sodium Azide (NaN3)
30% Glycerol
Carrier Free
Yes
Immunogen
A synthetic peptide containing human SPT16
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
Q9Y5B9
Entrez Gene
SUPT16H
GO Terms
GO:0006260 DNA replication
GO:0005525 GTP binding
GO:0005654 nucleoplasm
GO:0005694 chromosome
GO:0006281 DNA repair
GO:0006337 nucleosome disassembly
GO:0006368 transcription elongation from RNA polymerase II promoter
GO:0008159 positive transcription elongation factor activity
GO:0016032 viral reproduction
GO:0050434 positive regulation of viral transcription
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products