SMPD1 antibody
Rabbit anti SMPD1 antibody recognizes sphingomyelin phosphodiesterase, also known as ASM, Sphingomyelin Phosphodiesterase, ASMASE and NPD.
It is a lipid hydrolase that converts sphingomyelin to the bioactive messenger ceramide, and is activated by a number of molecules including TNF-alpha and LPS (Rozanova et al. 2010). Mutations in the SMPD1 gene resulting in deficient ASM production cause Niemann-Pick disease, one type of which causes lethality within three years of life (Schuchman 2007). SMPD1 mutations have also been implicated in sporadic Parkinson's disease (Mao et al. 2017). A role of ASM on mood regulation has been suggested, with high ASM expression implicated in major depressive episodes (Gulbins et al. 2015).
It is a lipid hydrolase that converts sphingomyelin to the bioactive messenger ceramide, and is activated by a number of molecules including TNF-alpha and LPS (Rozanova et al. 2010). Mutations in the SMPD1 gene resulting in deficient ASM production cause Niemann-Pick disease, one type of which causes lethality within three years of life (Schuchman 2007). SMPD1 mutations have also been implicated in sporadic Parkinson's disease (Mao et al. 2017). A role of ASM on mood regulation has been suggested, with high ASM expression implicated in major depressive episodes (Gulbins et al. 2015).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to SMPD1 was raised by repeated immunization of rabbits with highly purified antigen
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Recombinant protein of human SMPD1 / ASM
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P17405
- Entrez Gene
- SMPD1
- GO Terms
- GO:0005764 lysosome
- GO:0004767 sphingomyelin phosphodiesterase activity
- GO:0007165 signal transduction
- GO:0006685 sphingomyelin catabolic process
- GO:0007399 nervous system development
- GO:0008219 cell death
- GO:0016798 hydrolase activity, acting on glycosyl bonds
- Regulatory
- For research purposes only
Applications of SMPD1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
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