Rabbit anti SMPD1 antibody recognizes sphingomyelin phosphodiesterase, also known as ASM, Sphingomyelin Phosphodiesterase, ASMASE and NPD.
It is a lipid hydrolase that converts sphingomyelin to the bioactive messenger ceramide, and is activated by a number of molecules including TNF-alpha and LPS (Rozanova et al. 2010). Mutations in the SMPD1 gene resulting in deficient ASM production cause Niemann-Pick disease, one type of which causes lethality within three years of life (Schuchman 2007). SMPD1 mutations have also been implicated in sporadic Parkinson's disease (Mao et al. 2017). A role of ASM on mood regulation has been suggested, with high ASM expression implicated in major depressive episodes (Gulbins et al. 2015).
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to SMPD1 was raised by repeated immunization of rabbits with highly purified antigen
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Recombinant protein of human SMPD1 / ASM
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
sphingomyelin phosphodiesterase activity
sphingomyelin catabolic process
nervous system development
hydrolase activity, acting on glycosyl bonds
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of SMPD1 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available