SMPD1 antibody

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Rabbit anti SMPD1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP3001
Datasheet
WB
SDS
50 µl loader

Rabbit anti SMPD1 antibody recognizes sphingomyelin phosphodiesterase, also known as ASM, Sphingomyelin Phosphodiesterase, ASMASE and NPD.

It is a lipid hydrolase that converts sphingomyelin to the bioactive messenger ceramide, and is activated by a number of molecules including TNF-alpha and LPS (Rozanova et al. 2010). Mutations in the SMPD1 gene resulting in deficient ASM production cause Niemann-Pick disease, one type of which causes lethality within three years of life (Schuchman 2007). SMPD1 mutations have also been implicated in sporadic Parkinson's disease (Mao et al. 2017). A role of ASM on mood regulation has been suggested, with high ASM expression implicated in major depressive episodes (Gulbins et al. 2015).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to SMPD1 was raised by repeated immunization of rabbits with highly purified antigen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Recombinant protein of human SMPD1 / ASM

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P17405
Entrez Gene
SMPD1
GO Terms
GO:0005764 lysosome
GO:0004767 sphingomyelin phosphodiesterase activity
GO:0007165 signal transduction
GO:0006685 sphingomyelin catabolic process
GO:0007399 nervous system development
GO:0008219 cell death
GO:0016798 hydrolase activity, acting on glycosyl bonds
Regulatory
For research purposes only

Applications of SMPD1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg