SMARCE1 antibody

Rabbit anti Human SMARCE1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
SMARCE1

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New AHP3102
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SDS Safety Datasheet SDS
WB 0.1 ml loader
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loader
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Rabbit anti Human SMARCE1 antibody recognizes SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1.

SMARCE1 forms part of the ATP-dependent chromatin remodeling complex SWI/SNF, required for transcriptional activation of genes normally inhibited by chromatin. Mutations in the genes encoding members of this complex, including SMARCE1, have been observed in a variety of cancers (Biegel et al. 2014).

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human SMARCE1 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.035% Sodium Azide (NaN3)
30% Glycerol
Carrier Free
Yes
Immunogen
A peptide within the C-terminal region of human SMARCE1
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
Q969G3
Entrez Gene
SMARCE1
GO Terms
GO:0005515 protein binding
GO:0000228 nuclear chromosome
GO:0003677 DNA binding
GO:0003682 chromatin binding
GO:0003713 transcription coactivator activity
GO:0006337 nucleosome disassembly
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0007399 nervous system development
GO:0008080 N-acetyltransferase activity
GO:0016514 SWI/SNF complex
GO:0016568 chromatin modification
GO:0017053 transcriptional repressor complex
GO:0045892 negative regulation of transcription, DNA-dependent
GO:0047485 protein N-terminus binding
GO:0071564 npBAF complex
GO:0071565 nBAF complex
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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