RPB2 antibody
Rabbit anti Human RPB2
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- RPB2
Antibody Quality and Performance Guarantee
| Rabbit anti Human RPB2 antibody recognizes DNA-directed RNA polymerase II subunit RPB2. RPB2 is the second largest subunit of RNA polymerase II, the polymerase responsible for the synthesis of messenger RNA in eukaryotic cells. The RPB2 subunit combines with other polymerase subunits to form a structure within RNA polymerase II that maintains the contact between the DNA and the newly synthesized RNA within the active site of the enzyme (Acker et al. 1997). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human RPB2 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.035% Sodium Azide (NaN3)
30% Glycerol - Carrier Free
- Yes
- Immunogen
- A peptide within the N-terminal region of human RPB2
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoprecipitation |  |
5 µl per IP | 10 µl per IP |
| Western Blotting | |
1/500 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
- UniProt
- P30876
- Entrez Gene
- POLR2B
- GO Terms
- GO:0005515 protein binding
- GO:0000398 nuclear mRNA splicing, via spliceosome
- GO:0003677 DNA binding
- GO:0003899 DNA-directed RNA polymerase activity
- GO:0046872 metal ion binding
- GO:0005665 DNA-directed RNA polymerase II, core complex
- GO:0006283 transcription-coupled nucleotide-excision repair
- GO:0006367 transcription initiation from RNA polymerase II promoter
- GO:0006368 transcription elongation from RNA polymerase II promoter
- View More GO Terms
- GO:0006370 mRNA capping
- GO:0016032 viral reproduction
- GO:0032549 ribonucleoside binding
- GO:0050434 positive regulation of viral transcription
AHP3096
160751
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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