RPB2 antibody

Rabbit anti Human RPB2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
RPB2

Product Code Applications Pack Size List Price Your Price Qty
AHP3096
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SDS Safety Datasheet SDS
IP WB 0.1 ml loader
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Rabbit anti Human RPB2 antibody recognizes DNA-directed RNA polymerase II subunit RPB2.

RPB2 is the second largest subunit of RNA polymerase II, the polymerase responsible for the synthesis of messenger RNA in eukaryotic cells. The RPB2 subunit combines with other polymerase subunits to form a structure within RNA polymerase II that maintains the contact between the DNA and the newly synthesized RNA within the active site of the enzyme (Acker et al. 1997).

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human RPB2 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.035% Sodium Azide (NaN3)
30% Glycerol
Carrier Free
Yes
Immunogen
A peptide within the N-terminal region of human RPB2
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation 5 µl per IP 10 µl per IP
Western Blotting 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
P30876
Entrez Gene
POLR2B
GO Terms
GO:0005515 protein binding
GO:0000398 nuclear mRNA splicing, via spliceosome
GO:0003677 DNA binding
GO:0003899 DNA-directed RNA polymerase activity
GO:0046872 metal ion binding
GO:0005665 DNA-directed RNA polymerase II, core complex
GO:0006283 transcription-coupled nucleotide-excision repair
GO:0006367 transcription initiation from RNA polymerase II promoter
GO:0006368 transcription elongation from RNA polymerase II promoter
GO:0006370 mRNA capping
GO:0016032 viral reproduction
GO:0032549 ribonucleoside binding
GO:0050434 positive regulation of viral transcription
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products