RNA Pol II CTD (pThr4) antibody
Rabbit anti Human RNA Pol II CTD (pThr4)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- RNA Pol II CTD (pThr4)
Antibody Quality and Performance Guarantee
| Rabbit anti Human RNA pol II CTD (pThr4) antibody recognizes RNA polymerase II, also known as RNAP II and pol II, when phosphorylated at threonine 4 of the carboxyl-terminal repeat domain. RNA pol II is a 550 kDa enzyme complex made up of 12 subunits that transcribes DNA into precursors of messenger RNA (mRNA), snRNA, and microRNA (Kornburg, 1999) (Sims et al. 2004). The carboxyl-terminal repeat domain (CTD) of RNA pol II is reversibly phosphorylated in during the transcription cycle (Phatnani and Greenleaf, 2006). Phosphorylation of the CTD of RNA pol II at threonine 4 has been shown to facilitate histone mRNA 3' end processing (Hsin et al. 2011). Phosphorylation of the carboxyl-terminal repeat domain of RNA polymerase II at threonine 4 occurs at the 3' end of most human genes and is necessary for transcription elongation (Hintermair et al. 2012). Wide species cross-reactivity is expected from Rabbit anti Human RNA pol II CTD (pThr4) based on sequence. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Broad Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human RNA pol II CTD (pThr4) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.035% Sodium Azide (NaN3)
30% Glycerol - Carrier Free
- Yes
- Immunogen
- A peptide containing the RNA pol II C-terminal sequence phosphorylated at threonine 4
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1 µg/ml | 2 µg/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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- Synonyms
- RPB1
- UniProt
- P24928
- Entrez Gene
- POLR2A
- GO Terms
- GO:0000398 nuclear mRNA splicing, via spliceosome
- GO:0003677 DNA binding
- GO:0003899 DNA-directed RNA polymerase activity
- GO:0003968 RNA-directed RNA polymerase activity
- GO:0046872 metal ion binding
- GO:0005665 DNA-directed RNA polymerase II, core complex
- GO:0006283 transcription-coupled nucleotide-excision repair
- GO:0006355 regulation of transcription, DNA-dependent
- GO:0006367 transcription initiation from RNA polymerase II promoter
- View More GO Terms
- GO:0006368 transcription elongation from RNA polymerase II promoter
- GO:0006370 mRNA capping
- GO:0016032 viral reproduction
- GO:0031625 ubiquitin protein ligase binding
- GO:0050434 positive regulation of viral transcription
AHP3100
160785
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