RNA Pol II CTD (pSer5) antibody

Rabbit anti Human RNA Pol II CTD (pSer5)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
RNA Pol II CTD (pSer5)

Product Code Applications Pack Size List Price Your Price Qty
AHP3099
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
Ch WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Rabbit anti Human RNA pol II CTD (pSer5) antibody recognizes RNA polymerase II, also known as RNAP II and pol II, when phosphorylated at serine 5 of the carboxyl-terminal repeat domain.

RNA pol II is a 550 kDa enzyme complex made up of 12 subunits that transcribes DNA into precursors of messenger RNA (mRNA), snRNA, and microRNA (Kornburg, 1999) (Sims et al. 2004). The carboxyl-terminal repeat domain (CTD) of RNA pol II is reversibly phosphorylated in during the transcription cycle. It is thought that phosphorylation of the CTD of RNA pol II at serine 5 facilitates the formation of 3' ends of messages (Phatnani and Greenleaf, 2006). Phosphorylation of serine 5 of the carboxyl-terminal repeat domain of RNA pol II facilitates the recruitment of the capping enzyme (Egloff and Murphy, 2008).

Wide species cross-reactivity is expected from Rabbit anti Human RNA pol II CTD (pSer5) based on sequence.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Broad Expected from Sequence
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human RNA pol II CTD (pSer5) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography on Protein A.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.035% Sodium Azide (NaN3)
30% Glycerol
Carrier Free
Yes
Immunogen
A synthetic peptide containing the RNA pol II C-terminal domain phosphorylated at serine 5
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Chromatin Immunoprecipitation 4 µg per ChIP 10 µg per ChIP
Western Blotting 0.5 µg/ml 2 µg/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Synonyms
RPB1
UniProt
P24928
Entrez Gene
POLR2A
GO Terms
GO:0000398 nuclear mRNA splicing, via spliceosome
GO:0003677 DNA binding
GO:0003899 DNA-directed RNA polymerase activity
GO:0003968 RNA-directed RNA polymerase activity
GO:0046872 metal ion binding
GO:0005665 DNA-directed RNA polymerase II, core complex
GO:0006283 transcription-coupled nucleotide-excision repair
GO:0006355 regulation of transcription, DNA-dependent
GO:0006367 transcription initiation from RNA polymerase II promoter
GO:0006368 transcription elongation from RNA polymerase II promoter
GO:0006370 mRNA capping
GO:0016032 viral reproduction
GO:0031625 ubiquitin protein ligase binding
GO:0050434 positive regulation of viral transcription