PUMA antibody

Rabbit anti PUMA

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
PUMA

Product Code Applications Pack Size List Price Your Price Qty
AHP727
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Rabbit anti PUMA antibody recognizes the p53 - upregulated modulator of apoptosis, also known as Bcl-2 binding component 3, JFY-1 or Bcl-2. PUMA is a 193 amino acid ~20 kDa pro-apoptotic member of the BCL-2 family.

Bcl-2 binding component 3 is localized to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53 (Follis et al. 2013). Four isoforms of PUMA have been described due to alternative splicing. Rabbit anti PUMA antibody is expected to recognize the canonical isoform 1, also known as PUMA alpha, it is not expected to recognize any of the other isoforms of PUMA.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human Bcl-2 binding component 3 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
Synthetic peptide ARARQEGSSPEPVEG corresponding to the N-terminus of human Bcl-2 binding component 3.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 10 ug/ml
Western Blotting 2ug/ml 4ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human breast carcinoma
Western Blotting
AHP727 detects a band of approximately 23kDa in K562 cell lysates.

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References for PUMA antibody

  1. Naderi, E.H. et al. (2009) Activation of cAMP signaling inhibits DNA damage-induced apoptosis in BCP-ALL cells through abrogation of p53 accumulation.
    Blood. 114 (3): 608-18.

Further Reading

  1. Nakano, K. & Vousden, K.H. (2001) PUMA, a novel proapoptotic gene, is induced by p53.
    Mol Cell. 7 (3): 683-94.

Synonyms
JFY-1
RRID
AB_324241
UniProt
Q9BXH1
Entrez Gene
BBC3
GO Terms
GO:0005515 protein binding
GO:0001836 release of cytochrome c from mitochondria
GO:0005829 cytosol
GO:0005741 mitochondrial outer membrane
GO:0006919 activation of caspase activity
GO:0008629 induction of apoptosis by intracellular signals
GO:0008633 activation of pro-apoptotic gene products
GO:0045926 negative regulation of growth
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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