PUMA antibody
Rabbit anti PUMA
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- PUMA
Rabbit anti PUMA antibody recognizes the p53 - upregulated modulator of apoptosis, also known as Bcl-2 binding component 3, JFY-1 or Bcl-2. PUMA is a 193 amino acid ~20 kDa pro-apoptotic member of the BCL-2 family. Bcl-2 binding component 3 is localized to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53 (Follis et al. 2013). Four isoforms of PUMA have been described due to alternative splicing. Rabbit anti PUMA antibody is expected to recognize the canonical isoform 1, also known as PUMA alpha, it is not expected to recognize any of the other isoforms of PUMA. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human Bcl-2 binding component 3 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide - Immunogen
- Synthetic peptide ARARQEGSSPEPVEG corresponding to the N-terminus of human Bcl-2 binding component 3.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunohistology - Paraffin 1 | 10 ug/ml | ||
Western Blotting | 2ug/ml | 4ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Histology Positive Control Tissue
- Human breast carcinoma
- Western Blotting
- AHP727 detects a band of approximately 23kDa in K562 cell lysates.
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References for PUMA antibody
-
Naderi, E.H. et al. (2009) Activation of cAMP signaling inhibits DNA damage-induced apoptosis in BCP-ALL cells through abrogation of p53 accumulation.
Blood. 114 (3): 608-18.
Further Reading
-
Nakano, K. & Vousden, K.H. (2001) PUMA, a novel proapoptotic gene, is induced by p53.
Mol Cell. 7 (3): 683-94.
- Synonyms
- JFY-1
- RRID
- AB_324241
- UniProt
- Q9BXH1
- Entrez Gene
- BBC3
- GO Terms
- GO:0005515 protein binding
- GO:0001836 release of cytochrome c from mitochondria
- GO:0005829 cytosol
- GO:0005741 mitochondrial outer membrane
- GO:0006919 activation of caspase activity
- GO:0008629 induction of apoptosis by intracellular signals
- GO:0008633 activation of pro-apoptotic gene products
- GO:0045926 negative regulation of growth
AHP727
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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