PTK7 antibody

Rabbit anti Human PTK7 (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
PTK7
Region
(N-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP1440
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SDS Safety Datasheet SDS
P * WB 0.1 mg loader
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Rabbit anti Human PTK7 antibody recognizes the N-terminal region of human Protein tyrosine kinase-like 7 (PTK7), a member of the receptor tyrosine kinase family, otherwise known as Colon Carcinoma Kinase 4 (CCK4). PTK7 is highly expressed in the kidney, liver, lung, pancreas and melanocytes with weaker expression being detected in the thyroid gland, ovaries, brain, heart and skeletal muscle.

PTK7 may play a role in embryonic development. It is thought to function as a cell adhesion molecule and as a novel regulator of the planar cell polarity (PCP) pathway in vertebrates.

PTK7 may be involved in the tumour progression. A loss of PTK7 expression has been observed in melanoma cell lines, whereas increased levels of PTK7 have been detected in high-throughput acute myeloid leukaemia samples. In addition, PTK7 is not expressed in normal colon but has been detected in colon carcinomas and as such is a potential marker for tumour progression.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Antisera to human PTK7 (NT) were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
Immunogen
A 15 amino acid peptide located near the N-terminal region of human PTK7.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1
Western Blotting 0.5 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1440 detects a band of approximately 86kDa in colon tissue lysates.

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Further Reading

  1. Lu, X. et al. (2004) PTK7/CCK-4 is a novel regulator of planar cell polarity in vertebrates.
    Nature. 430 (6995): 93-8.
  2. Easty, D.J. et al. (1997) Loss of expression of receptor tyrosine kinase family genes PTK7 and SEK in metastatic melanoma.
    Int J Cancer. 71 (6): 1061-5.

Synonyms
CCK-4
RRID
AB_2174361
UniProt
Q13308
Entrez Gene
PTK7
View more products with PTK7 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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