PPAR Alpha antibody

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Rabbit anti Human PPAR Alpha (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1271 E WB 50 µg
Rabbit anti PPAR alpha antibody detects an epitope within the N-terminal region of human peroxisome proliferator-activated receptor, alpha (PPARA), a member of the nuclear hormone receptor subfamily. Upon activation, PPARA forms heterodimers with retinoid X receptors (RXRs), and functions to regulate transcription of specific gene sequences. In particular, PPARA controls genes involved in the peroxisomal and mitochondrial beta oxidation pathways, fatty acid uptake, and triglyceride catabolism in the liver and skeletal muscle.

PPARA also has anti-inflammatory and anti-proliferative effects and prevents the proatherogenic effects of cholesterol accumulation in macrophages by stimulating cholesterol efflux.

PPARA is the main target of fibrate drugs; hypolipidemic agents used in the treatment of dyslipidemia and the prevention of cardiovascular disease. They could also be used for the treatment of type II diabetes.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human PPAR alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01%Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to amino acids 1-18 of human PPAR alpha.
Approx. Protein Concentrations
IgG concentration 0.98mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q07869 Related reagents
Entrez Gene
PPARA Related reagents
GO Terms
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003706 ligand-regulated transcription factor activity
GO:0003707 steroid hormone receptor activity
GO:0005654 nucleoplasm
GO:0008289 lipid binding
GO:0006631 fatty acid metabolic process
GO:0008144 drug binding
GO:0008270 zinc ion binding
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010745 negative regulation of macrophage derived foam cell differentiation
GO:0010871 negative regulation of receptor biosynthetic process
GO:0010887 negative regulation of cholesterol storage
GO:0010891 negative regulation of sequestering of triglyceride
GO:0015908 fatty acid transport
GO:0016563 transcription activator activity
GO:0031624 ubiquitin conjugating enzyme binding
GO:0032000 positive regulation of fatty acid beta-oxidation
GO:0032099 negative regulation of appetite
GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
GO:0043565 sequence-specific DNA binding
GO:0045820 negative regulation of glycolysis
GO:0072363 regulation of glycolysis by positive regulation of transcription from an RNA polymerase II promoter
GO:0072366 regulation of cellular ketone metabolic process by positive regulation of transcription from an RNA polymerase II promoter
GO:0072369 regulation of lipid transport by positive regulation of transcription from an RNA polymerase II promoter
Regulatory
For research purposes only

Applications of PPAR Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/8000 1/32000
Western Blotting 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1271 detects a band of approximately 52kDa in 3T3 whole cell lysate.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Haluzík, M.M. & Haluzík, M. (2006) PPAR-alpha and insulin sensitivity.
    Physiol Res. 55 (2): 115-22.
  2. Lefebvre, P. et al. (2006) Sorting out the roles of PPAR alpha in energy metabolism and vascular homeostasis.
    J Clin Invest. 116 (3): 571-80.