Rabbit anti PPAR alpha antibody detects an epitope within the N-terminal region of human peroxisome proliferator-activated receptor, alpha (PPARA), a member of the nuclear hormone receptor subfamily. Upon activation, PPARA forms heterodimers with retinoid X receptors (RXRs), and functions to regulate transcription of specific gene sequences. In particular, PPARA controls genes involved in the peroxisomal and mitochondrial beta oxidation pathways, fatty acid uptake, and triglyceride catabolism in the liver and skeletal muscle.
PPARA also has anti-inflammatory and anti-proliferative effects and prevents the proatherogenic effects of cholesterol accumulation in macrophages by stimulating cholesterol efflux.
PPARA is the main target of fibrate drugs; hypolipidemic agents used in the treatment of dyslipidemia and the prevention of cardiovascular disease. They could also be used for the treatment of type II diabetes.
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human PPAR alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Synthetic peptide corresponding to amino acids 1-18 of human PPAR alpha.
- Approx. Protein Concentrations
- IgG concentration 0.98mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
sequence-specific DNA binding transcription factor activity
ligand-regulated transcription factor activity
steroid hormone receptor activity
fatty acid metabolic process
zinc ion binding
positive regulation of gene-specific transcription from RNA polymerase II promoter
negative regulation of gene-specific transcription from RNA polymerase II promoter
negative regulation of macrophage derived foam cell differentiation
negative regulation of receptor biosynthetic process
negative regulation of cholesterol storage
negative regulation of sequestering of triglyceride
fatty acid transport
transcription activator activity
ubiquitin conjugating enzyme binding
positive regulation of fatty acid beta-oxidation
negative regulation of appetite
regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
sequence-specific DNA binding
negative regulation of glycolysis
regulation of glycolysis by positive regulation of transcription from an RNA polymerase II promoter
regulation of cellular ketone metabolic process by positive regulation of transcription from an RNA polymerase II promoter
regulation of lipid transport by positive regulation of transcription from an RNA polymerase II promoter
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of PPAR Alpha antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1271 detects a band of approximately 52kDa in 3T3 whole cell lysate.
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Secondary Antibodies Available
Useful Reagents Available