PLGF antibody

Rabbit anti Human PLGF

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
PLGF

Product Code Applications Pack Size List Price Your Price Qty
AHP1381
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E P * WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Rabbit anti human PlGF antibody recognizes human placental growth factor (PlGF). PlGF is a dimeric glycoprotein and member of the vascular endothelial growth factor (VEGF) family, involved in angiogenesis, and the stimulation of endothelial cell proliferation and migration.

The three isoforms PlGF-1, PlGF-2 and PlGF-3 are present in most placental tissues and act through binding to the VEGFR-1/FLT1 receptor. The involvement of PlGF in angiogenesis has made PlGF a focus area in the study of inflammatory diseases such as rheumatoid arthritis and psoriasis, and PlGF is used as a biochemical marker in cardiovascular disease, and also detected at reduced levels during pre-eclampsia (Krauss et al. 2004).

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antisera to human PlGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant human PLGF.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Immunohistology - Paraffin 1 2.0ug/ml
Western Blotting 0.1 0.2ug/ml
  1. 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
This purified human PLGF antibody may be used in Western Blotting applications under either reducing or non-reducing conditions.

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References for PLGF antibody

  1. Maglione, D. et al. (1991) Isolation of a human placenta cDNA coding for a protein related to the vascular permeability factor.
    Proc Natl Acad Sci U S A. 88 (20): 9267-71.

Synonyms
Placental Growth Factor
RRID
AB_2161051
UniProt
P49763
Entrez Gene
PGF
GO Terms
GO:0001525 angiogenesis
GO:0016020 membrane
GO:0007267 cell-cell signaling
GO:0008083 growth factor activity
GO:0008201 heparin binding
GO:0030154 cell differentiation
GO:0048010 vascular endothelial growth factor receptor signaling pathway
GO:0051781 positive regulation of cell division
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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